Molecular Detection of Circulating Cancer Cells for Early Diagnosis of Breast Cancer

2001 ◽  
Author(s):  
Monica M. Reinholz ◽  
Patrick C. Roche
Keyword(s):  
Breast Cancer ◽  
Early Diagnosis ◽  
Cancer Cells ◽  
Molecular Detection ◽  
Circulating Cancer Cells

scholarly journals Evaluation of a Panel of Tumor Markers for Molecular Detection of Circulating Cancer Cells in Women with Suspected Breast Cancer

2005 ◽  
Vol 11 (10) ◽  
pp. 3722-3732 ◽  
Author(s):  
Monica M. Reinholz ◽  
Andrea Nibbe ◽  
Leslie M. Jonart ◽  
Kathleen Kitzmann ◽  
Vera J. Suman ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Tumor Markers ◽  
Molecular Detection ◽  
Circulating Cancer Cells

Molecular Detection of Circulating Cancer Cells

2003 ◽  
Vol 21 (4) ◽  
pp. 320-325 ◽  
Author(s):  
Bela Molnar ◽  
Ferenc Sipos ◽  
Orsolya Galamb ◽  
Zsolt Tulassay
Keyword(s):  
Cancer Cells ◽  
Molecular Detection ◽  
Circulating Cancer Cells

scholarly journals Circulating cancer cells in division in an early breast cancer patient

2011 ◽  
Vol 22 (9) ◽  
pp. 2150-2151 ◽  
Author(s):  
M.J. Serrano ◽  
R. Nadal ◽  
J.A. Lorente ◽  
M. Salido ◽  
R. Rodríguez ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patient ◽  
Breast Cancer Patient ◽  
Cancer Cells ◽  
Early Breast Cancer ◽  
Early Breast Cancer Patient ◽  
Circulating Cancer Cells

Serial monitoring for circulating cancer cells in blood samples of stage 1–4 breast cancer patients

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 623-623
Author(s):  
K. H. Tkaczuk ◽  
N. S. Tait ◽  
K. Chua ◽  
F. Feldman ◽  
S. A. Lesko ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Primary Tumor ◽  
Receptor Expression ◽  
Breast Cancer Patients ◽  
Blood Samples ◽  
Circulating Cancer Cells ◽  
Her 2 ◽  
Stage 1

623 Serial monitoring for presence, number & characterization of circulating cancer cells (CCC) may provide valuable information that may be relevant to prognosis and treatment outcomes of breast cancer patients (BCP). We conducted a serial blood sampling study at the University of Maryland in BCP with stage 1–4 breast carcinoma. 15–20 ml of venous blood were collected before the start of systemic therapy and periodically thereafter & processed using negative selection method with double-gradient centrifugation & magnetic cell sorting to remove WBCs. Digital images of FITC-positive epithelial cells were acquired with a fluorescence microscope & counted. CCC from 41 patients (Pts) were also stained with Trastuzu-mAb-532 to quantify the HER-2/neu cell surface receptor expression relative to a fluorescence standard. 105 Pts were accrued & 415 blood samples tested (median number of samples/pt; 4 (1–8). During the 24 mos. monitoring period CCC were detected in 57 of 105 pts (54%). The Table below shows that presence of >10 CCC/sample is associated with decreased survival and increased probability of having metastatic disease.(Exact chi-square test for presence vs. absence of metastatses in A, B, C, D groups, P < 0.0001; Fisher’s exact test to compare individual groups: for B vs C+ D, P < 0.001; B vs C, P=0.001). HER-2/neu expression was assessed in CCC of 25 pts (minimum of 4 CCC per sample) as compared with strongly HER-2/neu positive control cell line SKBR-3. 10 Pts were positive & 15 negative for HER-2/neu over-expression in CCC. CCC data & primary tumor data concurred in 6 of 7 Her-2/neu primary tumor tissue positive Pts & in 12 of 13 Her-2/neu primary tissue negative Pts. For 5 Pts tissue data was not available. Conclusions: Increasing CCC numbers/sample appear to correlate with adverse outcome of BCP. Our CCC Test may provide valuable information about prognosis of stage 1–4 BCP. HER-2/neu expression could be quantified in individual CCC & concurred with primary tumor data in 90% of Pts. Supported by NCI Grant CA081903 [Table: see text] [Table: see text]

Capture of EpCAM-negative and vimentin-positive circulating cancer cells (CCCs) from blood of metastatic breast cancer patients using ApoStream.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. e21029-e21029
Author(s):  
Christopher Neal ◽  
Sujita Sukumaran ◽  
Vishal Gupta ◽  
Insiya Jafferji ◽  
Dave Hasegawa ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Cancer Progression ◽  
Laser Scanning ◽  
Metastatic Breast ◽  
Breast Cancer Patients ◽  
Mesenchymal Transition ◽  
Circulating Cancer Cells

e21029 Background: Up-regulation of epithelial mesenchymal transition (EMT) and the reduction of epithelial marker expression is associated with invasion, cancer progression, resistance to conventional therapies and poor prognosis. ApoStream, a novel continuous flow dielectrophoresis field-flow fractionation (DEP-FFF) device, was used to enable antibody-independent capture of circulating cancer cells (CCCs,also referred to as circulating tumor cells, CTC) for subsequent phenotyping of EMT markers. Methods: A side-by-side comparison of CellSearch and ApoStream was performed on 10 metastatic breast cancer patients. A multiplexed immunofluorescent assay and laser scanning cytometry analyses were used to unambiguously identify CK+/CD45–/DAPI+ CCCs and quantify their EpCAM and vimentin expression. Results: ApoStream recovered CK+/CD45–/DAPI+ CCCs from each breast cancer patient sample tested (mean=255 CCCs per 7.5 ml blood, see Table). ApoStream consistently recovered significantly higher number of CCCs compared to CellSearch (p=0.024). ApoStream recovered both EpCAM+ and EpCAM– CCCs in 50% and 90% of patients, respectively. Vimentin+ CCCs were isolated from 90% of patients. Conclusions: ApoStream’s higher capture efficiency demonstrated the majority of CCCs from breast cancer patients were EpCAM negative and vimentin-positive. ApoStream technology can be used to monitor CCCs undergoing EMT. [Table: see text]

Relationship between the molecular detection of circulating cancer cells and the angiogenesis in gastric cancer

2000 ◽  
Vol 118 (4) ◽  
pp. A1401
Author(s):  
Futoshi Miyazono ◽  
Shouji Natugoe ◽  
Yuuji Takebayashi ◽  
Akihiro Nakajo ◽  
Sumiya Ishigami ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Molecular Detection ◽  
Circulating Cancer Cells

Molecular detection of circulating cancer cells during surgery in patients with biliary-pancreatic cancer

1999 ◽  
Vol 177 (6) ◽  
pp. 475-479 ◽  
Author(s):  
Futoshi Miyazono ◽  
Sonshin Takao ◽  
Shoji Natsugoe ◽  
Keiichirou Uchikura ◽  
Fumio Kijima ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Molecular Detection ◽  
Circulating Cancer Cells
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