Purification and Analysis of a Recombinant Human Anti-Cholera Toxin B Antibody.

1998 ◽  
Author(s):  
Jessica Dang ◽  
Suzanne Kracke ◽  
Peter A. Emanuel ◽  
Michael J. Gostomski ◽  
Darrel E. Menking
Keyword(s):  
Cholera Toxin ◽  
Toxin B ◽  
Cholera Toxin B

scholarly journals Spray-Dried Formulation of Epicertin, a Recombinant Cholera Toxin B Subunit Variant That Induces Mucosal Healing

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 576
Author(s):  
Micaela A. Reeves ◽  
Joshua M. Royal ◽  
David A. Morris ◽  
Jessica M. Jurkiewicz ◽  
Nobuyuki Matoba ◽  
...  
Keyword(s):  
Gastric Acid ◽  
Cholera Toxin ◽  
Oral Formulation ◽  
Size Exclusion ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Enteric Coated ◽  
Spray Dried

Epicertin (EPT) is a recombinant variant of the cholera toxin B subunit, modified with a C-terminal KDEL endoplasmic reticulum retention motif. EPT has therapeutic potential for ulcerative colitis treatment. Previously, orally administered EPT demonstrated colon epithelial repair activity in dextran sodium sulfate (DSS)-induced acute and chronic colitis in mice. However, the oral dosing requires cumbersome pretreatment with sodium bicarbonate to conserve the acid-labile drug substance while transit through the stomach, hampering its facile application in chronic disease treatment. Here, we developed a solid oral formulation of EPT that circumvents degradation in gastric acid. EPT was spray-dried and packed into enteric-coated capsules to allow for pH-dependent release in the colon. A GM1-capture KDEL-detection ELISA and size-exclusion HPLC indicated that EPT powder maintains activity and structural stability for up to 9 months. Capsule disintegration tests showed that EPT remained encapsulated at pH 1 but was released over 180 min at pH 6.8, the approximate pH of the proximal colon. An acute DSS colitis study confirmed the therapeutic efficacy of encapsulated EPT in C57BL/6 mice upon oral administration without gastric acid neutralization pretreatment compared to vehicle-treated mice (p < 0.05). These results provide a foundation for an enteric-coated oral formulation of spray-dried EPT.

scholarly journals Crystal structure of cholera toxin B-pentamer bound to receptor GM1 pentasaccharide

1994 ◽  
Vol 3 (2) ◽  
pp. 166-175 ◽  
Author(s):  
Ethan A. Merritt ◽  
Steve Sarfaty ◽  
Focco Van Den Akker ◽  
Cécile L'Hoir ◽  
Joseph A. Martial ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Cholera Toxin ◽  
Toxin B ◽  
Cholera Toxin B

scholarly journals Nonspanning Bivalent Ligands as Improved Surface Receptor Binding Inhibitors of the Cholera Toxin B Pentamer

2004 ◽  
Vol 11 (9) ◽  
pp. 1205-1215 ◽  
Author(s):  
Jason C. Pickens ◽  
Daniel D. Mitchell ◽  
Jiyun Liu ◽  
Xiaojian Tan ◽  
Zhongsheng Zhang ◽  
...  
Keyword(s):  
Cholera Toxin ◽  
Receptor Binding ◽  
Bivalent Ligands ◽  
Toxin B ◽  
Cholera Toxin B ◽  
Surface Receptor ◽  
Binding Inhibitors

Synthesis of Lactose Dendrimers and Multivalency Effects in Binding to the Cholera Toxin B Subunit

2001 ◽  
Vol 2001 (24) ◽  
pp. 4685 ◽  
Author(s):  
Ioannis Vrasidas ◽  
Nico J. de Mol ◽  
Rob M. J. Liskamp ◽  
Roland J. Pieters
Keyword(s):  
Cholera Toxin ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit
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