The Effect of Hyperthermia on Intracellular Sodium Concentration in Isolated Human Cells. A Preliminary Report.

1996 ◽  
Author(s):  
S. L. Gaffin ◽  
M. S. Koratich ◽  
R. W. Hubbard
Keyword(s):  
Preliminary Report ◽  
Sodium Concentration ◽  
Human Cells ◽  
Intracellular Sodium ◽  
Intracellular Sodium Concentration

scholarly journals STUDY OF THE EFFECT OF HYPOTHERMIC CONSERVATION ON THE INTRACELLULAR SODIUM CONCENTRATION IN THE ENDOTHELIUM OF CORNEAL TRANSPLANTS

2018 ◽  
Vol 22 (4) ◽  
pp. 433-437
Author(s):  
G. S. Baturina ◽  
I. G. Palchikova ◽  
A. A. Konev ◽  
E. S. Smirnov ◽  
L. E. Katkova ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Plasma Membranes ◽  
Sodium Concentration ◽  
Intracellular Sodium ◽  
Sodium Balance ◽  
Human Cornea ◽  
Sodium Permeability ◽  
Custom Made ◽  
Intracellular Sodium Concentration ◽  
Cornea Endothelium

Endothelial keratoplasty has become the treatment of choice for corneal endothelial dysfunction. Advancements in the surgical treatment of corneal endothelial diseases depend on progress in graft conservation and its related advantages in assessing the suitability of grafts for transplantation. Transport of water and ions by cornea endothelium is important for the optic properties of cornea. In this work, we study the intracellular sodium concentration in cornea endothelial cells in samples of pig cornea that underwent hypothermic conservation for 1 and 10 days and endothelial cells of human cornea grafts after 10-day conservation. The concentration of intracellular sodium in preparations of endothelial cells was assayed using fluorescent dye SodiumGreen. The fluorescent images were analyzed with the custom-made computer program CytoDynamics. An increased level of intracellular sodium was shown in the endothelium after 10-day conservation in comparison with one-day conservation (pig samples). Sodium permeability of pig endothelial cell plasma membranes significantly decreased in these samples. Assessment of intracellular sodium in human cornea endothelium showed a higher level – as was in analogues pig samples of the corneal endothelium. The assay of the intracellular sodium balance concentration established in endothelial cells after hypothermic conservation in mediums L-15 and Optisol-GS showed a significant advantage of specialized me dium Optisol-GS. The balanced intracellular concentration after 10 days of hypothermic conservation was significantly lower in cells incubated at 4 °C in Optisol-GS (L-15, 128 ± 14,  n = 15; Optisol-GS, 108 ± 14, n = 11; mM, p < 0.001). Intracellular sodium concentration could be a useful parameter for assessing cornea endothelium cell viability.

scholarly journals Agonist-dependent Regulation of Renal Na+,K+-ATPase Activity Is Modulated by Intracellular Sodium Concentration

2002 ◽  
Vol 277 (13) ◽  
pp. 11489-11496 ◽  
Author(s):  
Riad Efendiev ◽  
Alejandro M. Bertorello ◽  
Ruben Zandomeni ◽  
Angel R. Cinelli ◽  
Carlos H. Pedemonte
Keyword(s):  
Atpase Activity ◽  
Sodium Concentration ◽  
Intracellular Sodium ◽  
Intracellular Sodium Concentration

scholarly journals Biological strandard for electron microprobe analysis of intracellular sodium concentration.

1976 ◽  
Vol 24 (6) ◽  
pp. 749-751 ◽  
Author(s):  
V S Sottiurai ◽  
R L Malvin ◽  
L F Allard ◽  
W C Bigelow
Keyword(s):  
Electron Microprobe ◽  
Microprobe Analysis ◽  
Electron Microprobe Analysis ◽  
Large Cell ◽  
Sodium Concentration ◽  
Intracellular Sodium ◽  
Cell Populations ◽  
Intracellular Sodium Concentration ◽  
Saline Solutions

A method is described for the determination of the intracellular concentration of sodium in individual cells using the electron microprobe analyzer. This method gives an accuracy equal to that obtained by using flame photometry on tissues with large cell populations. Intracellular sodium was precipitated in the cell by a fixative containing pyroantimonate. Cartilaginous needles from shark fins which were equilibrated in saline solutions of differing concentrations were used as biological standards.

Diphenylhydantoin and intracellular sodium concentration

Neurology ◽  
1969 ◽  
Vol 19 (4) ◽  
pp. 419-419 ◽  
Author(s):  
J. H. Pincus ◽  
M. D. Rawson
Keyword(s):  
Sodium Concentration ◽  
Intracellular Sodium ◽  
Intracellular Sodium Concentration

Intra-Erythrocyte Sodium and (Na+,K+-Activated)-ATPase Concentration and Urinary Aldosterone Excretion in Spontaneously Hypertensive Rats

1981 ◽  
Vol 60 (2) ◽  
pp. 229-232 ◽  
Author(s):  
G. Berglund ◽  
L. Sigström ◽  
S. Lundin ◽  
B. E. Karlberg ◽  
H. Herlitz
Keyword(s):  
Spontaneously Hypertensive Rats ◽  
Sodium Concentration ◽  
Intracellular Sodium ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Wistar Kyoto Rats ◽  
Significant Difference ◽  
Intracellular Sodium Concentration ◽  
Erythrocyte Sodium ◽  
Wistar Kyoto

1. Intra-erythrocyte sodium, potassium, ATP and (Na+,K+-activated)-ATPase concentrations and urinary aldosterone excretion were compared in 3-month-old spontaneously hypertensive rats (n = 11) and normotensive Wistar-Kyoto control rats (n = 11). 2. Spontaneously hypertensive rats exhibited significantly higher intra-erythrocyte sodium concentration (5.5 ± 1.3 vs 4.0 ± 1.1 mmol/l of erythrocytes, P < 0.01). No significant difference was found in intra-erythrocyte potassium, ATP or (Na+,K+-activated)-ATPase concentration. 3. Mean urinary aldosterone excretion was significantly lower in spontaneously hypertensive rats (66.3 ± 6.5 pmol/24 h) than in Wistar-Kyoto rats (90.5 ± 10.6 pmol/24 h, P < 0.01). No significant relationship between urinary aldosterone and intra-erythrocyte sodium concentration was found in spontaneously hypertensive or Wistar-Kyoto rats or in the pooled group. 4. These results are thus consistent with previous findings of an increased intracellular sodium concentration in spontaneously hypertensive rats, but do not support the hypothesis that aldosterone is a dominant regulator of intracellular sodium concentration.

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