Assaying the Mutagenic Potential of ELF Radiation through Reverse Mutagenesis via the Ames Test.

1996 ◽  
Author(s):  
Paul D. Moga ◽  
John W. Obringer
Keyword(s):  
Ames Test ◽  
Mutagenic Potential

scholarly journals A Promising Copper(II) Complex as Antifungal and Antibiofilm Drug against Yeast Infection

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1856 ◽  
Author(s):  
Fabiana Gomes da Silva Dantas ◽  
Adriana Araújo de Almeida-Apolonio ◽  
Renata Pires de Araújo ◽  
Lis Regiane Vizolli Favarin ◽  
Pamella Fukuda de Castilho ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Ames Test ◽  
Inhibitory Concentration ◽  
Coordination Complexes ◽  
Antifungal Property ◽  
Candida Spp ◽  
Mutagenic Potential ◽  
Yeast Infection ◽  
Limited Option ◽  
Potential Complex

The high mortality rate of candidemia and the limited option for the treatment of Candida spp. infection have been driving the search for new molecules with antifungal property. In this context, coordination complexes of metal ions and ligands appear to be important. Therefore, this study aimed to synthesize two new copper(II) complexes with 2-thiouracil and 6-methyl-2-thiouracil ligands and to evaluate their mutagenic potential and antifungal activity against Candida. The complexes were synthesized and characterized by infrared vibrational spectroscopy, CHN elemental analysis, UV-Vis experiments and ESI-HRMS spectrometry studies. The antifungal activity was evaluated by broth microdilution against 21 clinical isolates of Candida species. The mutagenic potential was evaluated by the Ames test. The complexes were Cu(Bipy)Cl2(thiouracil) (Complex 1) and Cu(Bipy)Cl2(6-methylthiouracil) (Complex 2). Complex 1 showed fungicidal and fungistatic activities against all isolates. Furthermore, the Minimum Inhibitory Concentration (MIC) from 31 to 125 µg/mL and inhibition percentage of 9.9% against the biofilms of C. krusei and C. glabrata were demonstrated. At the concentrations tested, complex 1 exhibited no mutagenic potential. Complex 2 and the free ligands exhibited no antifungal activity at the concentrations evaluated. Since complex 1 presented antifungal activity against all the tested isolates and no mutagenic potential, it could be proposed as a potential new drug for anti-Candida therapy.

scholarly journals β-Sitosterol oxidation products failed to show mutagenic potential in the Ames test

2007 ◽  
Vol 7 (S2) ◽  
Author(s):  
Karin Koschutnig ◽  
Suvi Kemmo ◽  
Anna-Maija Lampi ◽  
Vieno Piironen ◽  
Karl-Heinz Wagner
Keyword(s):  
Ames Test ◽  
Oxidation Products ◽  
Mutagenic Potential

Mutagenic potential of toluidine blue evaluated in the Ames test

1992 ◽  
Vol 279 (4) ◽  
pp. 255-259 ◽  
Author(s):  
Ann J. Dunipace ◽  
Rebecca Beaven ◽  
Timothy Noblitt ◽  
Li Yiming ◽  
Susan Zunt ◽  
...  
Keyword(s):  
Toluidine Blue ◽  
Ames Test ◽  
Mutagenic Potential

scholarly journals Antimicrobial and Antibiofilm Activities of 4,5-Dihydro-1H-pyrazole-1-carboximidamide Hydrochloride against Salmonella spp.

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Andressa C. Damim ◽  
Danilo Y. de Albuquerque ◽  
Fabiana G. S. Dantas ◽  
Fernanda Galvão ◽  
Melyssa Negri ◽  
...  
Keyword(s):  
Cell Viability ◽  
Biofilm Formation ◽  
Ames Test ◽  
Inhibitory Concentration ◽  
Vero Cells ◽  
Test Compound ◽  
Broth Microdilution ◽  
Salmonella Spp ◽  
Mutagenic Potential ◽  
Vero Cell Lines

In the present study, the antimicrobial and antibiofilm activities of two 4,5-dihydro-1H-pyrazole-1-carboximidamide hydrochloride, (trifluoromethyl) phenyl-substituted (compound 1) and bromophenyl-substituted (compound 2), were evaluated against four Salmonella spp. serotypes through broth microdilution and biofilm-forming activity. Further, the cytotoxicity of the compounds was evaluated by cell viability assays using cultures of HeLa and Vero cell lines, and the mutagenic potential was assessed by the Ames test. In the broth microdilution test, compound 1 inhibited 90% of the strains tested at the minimum inhibitory concentration of 62.5 μg mL−1. Furthermore, both compounds prevented biofilm formation, with a reduction of up to 5.2 log10. HeLa and Vero cells exhibited 100% viability in the presence of compound 1. In contrast, low cell viability was observed in the presence of 15 µg mL−1 of compound 2. Furthermore, no mutagenic potential was detected at any of the tested concentrations of compound 1.

Evaluation of the mutagenic potential of a combinational prophylactic transdermal patch by Ames test

2013 ◽  
Vol 28 (5-6) ◽  
pp. 322-326
Author(s):  
S. Banerjee ◽  
S. Singh ◽  
R. Policegoudra ◽  
P. Chattopadhyay ◽  
A. Ghosh ◽  
...  
Keyword(s):  
Ames Test ◽  
Transdermal Patch ◽  
Mutagenic Potential

scholarly journals Testing of the mutagenic potential of N-(γ-aminobuturyl)-1-aza-15-crown-5 hydrochloride in Ames test microplate modification

2019 ◽  
pp. 81-87
Author(s):  
M. Ya. Golovenko ◽  
V. B. Larionov ◽  
S. S. Basok ◽  
A. S. Reder
Keyword(s):  
Salmonella Typhimurium ◽  
Sodium Azide ◽  
Anticonvulsant Activity ◽  
Ames Test ◽  
Point Mutations ◽  
Gene Mutations ◽  
Metabolic Activation ◽  
Chemical Mutagenesis ◽  
Mutagenic Potential ◽  
Significant Development

In recent years, studies in the field of chemical mutagenesis have undergone significant development, due to the introduction of a large number of different chemicals and scientific advances in the creation and use of new test systems, allowing a complete assessment of both mutagens themselves and their metabolites. The aim of the work was to determine possible induction of gene mutations under influence of hydrochloride N-(γ-aminobuturil)-1-aza-4,7,10,13-tetraozacyclopentadecan (TOCPD), which has nootropic, anxiolytic and anticonvulsant activity. The ability of TOCPD to induce gene mutations was evaluated in Ames test on strains Salmonella typhimurium ТА 98 (frame shift mutations) and ТА 100 (substitution point mutations). The compound was used at concentrations of 10, 100, 250, 500 and 1000 μg/ml. Standard mutagens were 2-nitrofluoren for Salmonella typhimurium ТА 98 and sodium azide for Salmonella typhimurium ТА 100 in test without metabolic activation. In an activation variant a microsomal activating mixture was used (S9 mix). In tests with activation for both strains, 2-aminoantracene was used. The µAmes kit, Moltox (USA) and Muta-ChromoPlate kit (Canada) were used in the work. The results were evaluated by the number of wells with mutated cells with medium color changing from purple to yellow. The obtained data showed that in the control and according to the action of corresponding mutagens, the percentage of wells with mutated cells corresponded to the standard parameters determined by protocol of the microplate test. For the action of TOCPD compound, no gene mutations were detected in both S. typhimurium ТА 98 and ТА 100 strains within the concentrations used.

scholarly journals Mutagenic Potential of Artificial Athletic Field Crumb Rubber at Increased Temperatures

2015 ◽  
Vol 115 (2) ◽  
pp. 32 ◽  
Author(s):  
Michael J. Dorsey ◽  
Elizabeth Anderson ◽  
Oliva Ardo ◽  
Max Chou ◽  
Edward Farrow ◽  
...  
Keyword(s):  
Surface Temperature ◽  
Salmonella Typhimurium ◽  
Ambient Temperature ◽  
Ames Test ◽  
Crumb Rubber ◽  
Water Soluble ◽  
Mutagenic Potential ◽  
Athletic Fields ◽  
Athletic Field ◽  
Different Temperatures

Rubber tires contain several compounds that are known or suspected carcinogens.  Many carcinogens are mutagens, and fluctuation assays based on the Ames test can be used as an initial screen for mutagenic potential.  Granulated crumb rubber from recycled tires is commonly used in the creation of artificial athletic fields, and the surface temperature of these fields can reach levels far above the ambient temperature.  In this study, crumb rubber samples taken directly from four separate artificial athletic field surfaces were used to make leachates using water at different temperatures.  For each of these fields, leachates obtained in water at 70 ºC showed significant mutagenic potential (p ≤ .001) in Salmonella typhimurium fluctuation assays.  Leachates obtained in water at 40 ºC showed no mutagenic potential for any of the fields tested.  For one field, crumb rubber heated in water at temperatures as low as 50 ºC resulted in significant mutagenic potential (p ≤ 0.001).  Water used in an experiment designed to mimic the irrigation of an artificial athletic field also showed mutagenic potential (p ≤ 0.001) in a fluctuation assay.  These results suggest that at the higher temperatures that can exist on artificial athletic field surfaces, the crumb rubber infill on these artificial athletic fields can become the source of a water soluble agent with mutagenic potential in bacteria.

scholarly journals Evaluation Of The Mutagenic Potential Of The Polyphenol Extract From Blueberries In The Ames Test

2021 ◽  
Author(s):  
Z.T. Shulgau ◽  
S.D. Sergazy ◽  
A.M. Zhulikeeva ◽  
A.Y. Dautov ◽  
A.Y. Gulyayev ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Gene Mutation ◽  
Ames Test ◽  
Mutagenic Activity ◽  
Dose Range ◽  
Mutation Induction ◽  
Spontaneous Mutations ◽  
Mutagenic Potential ◽  
Dose Dependent Increase ◽  
Dose Dependent

In this research, mutagenic properties of blueberry polyphenol extract were studied in gene mutation induction test (Ames test) on four strains of Salmonella typhimurium TA98, TA100, TA1535, TA1537. None of the strains of Salmonella typhimurium showed statistically reliable dose-dependent increase in number of revertant colonies in the presence of investigated drug in the studied dose range from 4,0 to 40,0 mg/ml relative to baseline of spontaneous mutations. The blueberry extract does not have any mutagenic activity in the researched dose range in relation to TA98, TA100, TA1535, TA1537 strains of Salmonella typhimurium.

scholarly journals Synthesis and biological profiling of novel isocoumarin derivatives and related compounds

2021 ◽  
pp. 25-25
Author(s):  
Milena Simic ◽  
Slavica Eric ◽  
Ivan Boric ◽  
Annamaria Lubelska ◽  
Gniewomir Latacz ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Ames Test ◽  
The Other ◽  
Moderate Activity ◽  
Mutagenic Potential ◽  
Cyp Enzymes ◽  
Related Compounds

In continuation of our study of substituted isocoumarins a series of novel 3-azolyl isocoumarin and their thio derivatives including related lactone compounds was prepared and biologically profiled against C. albicans showing, in general, moderate activity with MIC values in range of 4-60 mg mL?1. Additional characterization of selected compounds was carried out by exploring their activity on CYP3A4 and CYP2D6 enzymes whilst experiments on mutagenicity were performed by AMES test. The representative isocoumarins 3b, 4a and 4b showed less inhibitory activity on CYP enzymes compared to the reference inhibitors, ketoconazole and quinidine. Compound 4a showed a higher mutagenic potential than the other two compounds. Further characterization included cytotoxicity profiling against normal MRC5 cells.

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