A Study of the Effects of High Power Pulsed 2450 MHz Microwaves, ELF modulated Microwaves, and ELF Fields on Human Lymphocytes and Selected Cell Lines

1993 ◽  
Author(s):  
Mays L. Swicord
Keyword(s):  
Cell Lines ◽  
High Power ◽  
Human Lymphocytes

scholarly journals Crude ethanol extract from babassu (Orbignya speciosa): cytotoxicity on tumoral and non-tumoral cell lines

2008 ◽  
Vol 80 (3) ◽  
pp. 467-476 ◽  
Author(s):  
Magdalena N. Rennó ◽  
Gleyce M. Barbosa ◽  
Patricia Zancan ◽  
Venicio F. Veiga ◽  
Celuta S. Alviano ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Human Lymphocytes ◽  
Ethanol Extract ◽  
Mouse Fibroblast ◽  
Maximal Effect ◽  
Nuclear Condensation ◽  
Popular Medicine ◽  
Tumoral Cell ◽  
Promising Source

Plant-derived substances have been considered as important sources of drugs, including antineoplasic agents. Babassu mesocarp is popularly used in Brazil as a food additive, and in popular medicine against several conditions, such as inflammations, menstrual pains and leukaemia. From babassu Orbignya speciosa (Mart.) Barb. Rodr. [Arecaceae (Palmae)] epicarp/mesocarp, an ethanol extract was prepared and named OSEME, which was tested on the viability,morphology and metabolism of several cell lines, such as the leukaemic cell lines, HL-60, K562 and the latter multidrug resistant counterpart K562-Lucena 1, the human breast cancer cell line MCF-7, the mouse fibroblast cell line 3T3-L1 and fresh human lymphocytes. OSEME promoted a dose-dependent decrease on the viability of all cells. This effect was much more pronounced on the tumoral cell lines than on non-tumoral cells, a phenomenon revealed by the dose of OSEME which promotes half of maximal effect (ID50). The decrease on viability was followed by shrinkage of cells, alteration on their morphology, and a markedly nuclear condensation. Curiously, stimulation of 6-phosphofructokinase activity (6.6-times) was observed on HL-60 cells, treated with OSEME, when compared to control treated with ethanol (vehicle). These results support evidences to suggest OSEME as a promising source of novel antineoplasic agents.

Comparative effects of selected antifolates on transforming human lymphocytes and on established human lymphoblastic cell lines

1976 ◽  
Vol 25 (17) ◽  
pp. 1947-1954 ◽  
Author(s):  
C.Choo Hoffmann ◽  
Yiu K. Ho ◽  
Raymond L. Blakley ◽  
John S. Thompson
Keyword(s):  
Cell Lines ◽  
Human Lymphocytes

scholarly journals Immunological analysis of a chromosomal antigen specific to human granulocytes

1982 ◽  
Vol 203 (1) ◽  
pp. 235-238 ◽  
Author(s):  
K C Ramage ◽  
J H J Dunn ◽  
A M Campbell
Keyword(s):  
Cell Lines ◽  
Hela Cells ◽  
Human Lymphocytes ◽  
Protein Antigen ◽  
Chromosomal Protein ◽  
Immunological Analysis ◽  
Fibrosarcoma Cells ◽  
Human Granulocytes ◽  
Human Fibrosarcoma Cells

A chromosomal protein antigen specific for human granulocytes is described. The antigen is present in large amounts in granulocytes and in granulocytic leukaemia. It is absent from HeLa cells, human fibrosarcoma cells and human lymphocytes. It is, however, present in small amounts in other human haemopoietic cell lines. The antigen binds tightly to DNA and requires the presence of DNA to react with the antibody.

Apoptotic and immunomodulatory effects of green tea extracts (GTE) on chemoresistant human tumor cells

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e22101-e22101
Author(s):  
D. P. Braun ◽  
D. M. Johnson ◽  
N. G. Katsantonis ◽  
K. Bhesaniya ◽  
E. D. Staren
Keyword(s):  
Cell Lines ◽  
Fas Ligand ◽  
Human Lymphocytes ◽  
Cell Cancer ◽  
Human Tumor ◽  
Tumor Proliferation ◽  
Immunomodulatory Effects ◽  
Inhibition Of Proliferation ◽  
Media Control ◽  
Apoptotic Genes

e22101 Background: GTE and Epigallocatechin 3-gallate (EGCG), the most abundant polyphenol in GTE, have been shown to exert inhibitory effects on carcinogenesis; modulatory effects on tumor proliferation and differentiation; and immunomodulatory effects on tumor immunity in different pre-clinical models. These pluripotent effects suggest that GTE may have clinical activity that could be exploited for treatment of chemo-insensitive but immunologically responsive tumors. Thus, the present study investigated the effects of EGCG on the proliferation and immunologic sensitivity of human renal cell cancer (RCC) and malignant melanoma (MM) cell lines. Methods: Human RCC (769-P) and MM (A375) cell lines were tested following incubation in media ± 21.8μM EGCG, a pharmacologically-achievable concentration produced by 8, 200mg capsules GTE daily. Tumor proliferation was assessed by MTS assay; lytic sensitivity to IL2-activated human peripheral blood lymphocytes (IL2PBL) by 51Chromium release assay; and gene expression by quantitative RT-PCR assay. Results: EGCG produced significant inhibition of proliferation of both RCC and MM cells (61.5% and 67.3% of media control values respectively; p<0.01 by 2-tailed, paired t test). EGCG also caused significantly increased expression (≥ 2 times (×) media control values) of pro-apoptotic genes in both RCC: BCL2L1 (5.4×); BCL2L10 (7.9×); BIK (2.9×); Caspase 5 (6.3×); and Caspase 14 (6.3×) and MM cells: Caspase8 (2X) and Card6 (2.5X). Unfortunately, EGCG pre-treatment also decreased the sensitivity of RCC (43% of control) and MM (53% of control) to IL2PBL-mediated lysis (p<0.01 respectively). This occured in association with significant upregulation of Fas ligand mRNA in RCC (6.3×) and TRAF2 mRNA in MM (33 ×). Conclusions: The results of this pre-clinical study demonstrate that EGCG exerts significant antiproliferative effects against human RCC and MM cells in vitro in association with increased expression of different pro-apoptotic genes. Unfortunately, EGCG-treated cells also demonstrated reduced sensitivity to lysis by IL2-activated human lymphocytes. Taken together, the results suggest that GTE may have activity in vivo in patients against chemoresistant RCC or MM but should not be used in conjunction with IL2 therapy. No significant financial relationships to disclose.

Xanthones as inhibitors of growth of human cancer cell lines and Their effects on the proliferation of human lymphocytes In Vitro

2002 ◽  
Vol 10 (12) ◽  
pp. 3725-3730 ◽  
Author(s):  
Madalena Pedro ◽  
Fátima Cerqueira ◽  
Maria Emı́lia Sousa ◽  
Maria São José Nascimento ◽  
Madalena Pinto
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Human Lymphocytes ◽  
Cancer Cell Lines ◽  
Human Cancer Cell ◽  
Human Cancer Cell Lines

scholarly journals Combinations of TLR Ligands: A Promising Approach in Cancer Immunotherapy

2013 ◽  
Vol 2013 ◽  
pp. 1-14 ◽  
Author(s):  
Saskia Stier ◽  
Claudia Maletzki ◽  
Ulrike Klier ◽  
Michael Linnebacher
Keyword(s):  
Tumor Growth ◽  
Tumor Cells ◽  
Cell Lines ◽  
Immune Cells ◽  
Human Lymphocytes ◽  
Growth Control ◽  
Poly I:C ◽  
The Impact

Toll-like receptors (TLRs), a family of pattern recognition receptors recognizing molecules expressed by pathogens, are typically expressed by immune cells. However, several recent studies revealed functional TLR expression also on tumor cells. Their expression is a two-sided coin for tumor cells. Not only tumor-promoting effects of TLR ligands are described but also direct oncopathic and immunostimulatory effects. To clarify TLRs’ role in colorectal cancer (CRC), we tested the impact of the TLR ligands LPS, Poly I:C, R848, and Taxol on primary human CRC cell lines (HROC40, HROC60, and HROC69)in vitroandin vivo(CT26). Taxol, not only a potent tumor-apoptosis-inducing, but also TLR4-activating chemotherapeutic compound, inhibited growth and viability of all cell lines, whereas the remaining TLR ligands had only marginal effects (R848 > LPS > Poly I:C). Combinations of the substances here did not improve the results, whereas antitumoral effects were dramatically boosted when human lymphocytes were added. Here, combining the TLR ligands often diminished antitumoral effects.In vivo, best tumor growth control was achieved by the combination of Taxol and R848. However, when combined with LPS, Taxol accelerated tumor growth. These data generally prove the potential of TLR ligands to control tumor growth and activate immune cells, but they also demonstrate the importance of choosing the right combinations.

scholarly journals The Adenovirus E3 RID Complex Protects Some Cultured Human T and B Lymphocytes from Fas-Induced Apoptosis

2002 ◽  
Vol 76 (19) ◽  
pp. 9716-9723 ◽  
Author(s):  
Adrienne L. McNees ◽  
C. T. Garnett ◽  
Linda R. Gooding
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Human Lymphocytes ◽  
Acute Infection ◽  
Receptor Internalization ◽  
Type I ◽  
Persistently Infected ◽  
Fas Signaling ◽  
T Cell Lines

ABSTRACT Human group C adenoviruses cause an acute infection in respiratory epithelia and establish a long-term or persistent infection, possibly in lymphocytes. The mechanism by which this persistence is maintained is unknown; however, it would require that persistently infected lymphocytes not be deleted. The adenovirus genome encodes proteins that prevent the immune system from eliminating the virus-infected cell, including the E3 receptor internalization and degradation (RID) complex. The RID complex prevents death of infected cells by blocking apoptosis initiated through death domain-containing receptors of the tumor necrosis factor receptor (TNFR) superfamily, including TNFR1 (L. R. Gooding, T. S. Ranheim, A. E. Tollefson, L. Aquino, P. Duerksen-Hughes, T. M. Horton, and W. S. Wold, J. Virol. 65:4114-4123, 1991), TNF-related apoptosis-inducing ligand receptors (TRAIL-R1 and -R2) (C. A. Benedict, P. S. Norris, T. I. Prigozy, J. L. Bodmer, J. A. Mahr, C. T. Garnett, F. Martinon, J. Tschopp, L. R. Gooding, and C. F. Ware, J. Biol. Chem. 276:3270-3278, 2001; A. E. Tollefson, K. Toth, K. Doronin, M. Kuppuswamy, O. A. Doronina, D. L. Lichtenstein, T. W. Hermiston, C. A. Smith, and W. S. Wold, J. Virol. 75:8875-8887, 2001), and Fas (J. Shisler, C. Yang, B. Walter, C. F. Ware, and L. R. Gooding, J. Virol. 71:8299-8306, 1997). Here, we test the ability of RID to protect human lymphocytes from apoptosis induced by ligation of Fas, a mechanism important for regulating lymphocyte populations. Using a retrovirus expressing RID to infect six human lymphocyte cell lines, we found that RID functions in the absence of other viral proteins to downregulate surface Fas on some, but not all, cell lines. Total cellular levels of Fas decrease as measured by Western blotting, and this loss of Fas correlates with protection from apoptosis induced by ligation of Fas in every cell line tested. Although in some cases, RID causes loss of only a fraction of surface Fas, the presence of RID completely blocks the immediate events downstream of Fas ligation (i.e., Fas-FADD association and caspase-8 cleavage) in susceptible cell lines. Nonetheless, the ability of RID to block Fas signaling is independent of the Fas signaling pathway used (type I or type II). Interestingly, among the four T-cell lines tested, RID caused loss of Fas in the two T-cell lines bearing a relatively immature phenotype, while having no activity in T cells with mature phenotypes. Collectively, these data suggest that RID functions to prevent apoptosis of some human lymphocytes by internalizing surface Fas receptors. It is possible that the expression of RID facilitates long-term infection by preventing Fas-mediated deletion of persistently infected lymphocytes.

Tumour cell lines induce interferon in human lymphocytes

Nature ◽  
1977 ◽  
Vol 270 (5638) ◽  
pp. 611-613 ◽  
Author(s):  
GIORGIO TRINCHIERI ◽  
DANIELA SANTOLI ◽  
BARBARA B. KNOWLES
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Human Lymphocytes ◽  
Tumour Cell Lines

Using Immortalized Human Lymphocytes Cell Lines to Screen the Genetic Markers of ADH5 to Formaldehyde

2012 ◽  
Vol 195-196 ◽  
pp. 465-468
Author(s):  
Juan Zhang ◽  
Li Hong Yin ◽  
Yue Pu Pu
Keyword(s):  
B Lymphocytes ◽  
Cell Lines ◽  
Genetic Markers ◽  
Human Lymphocytes ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Chinese Han ◽  
Efficient Tool ◽  
Genetic Biomarkers ◽  
Polymerase Chain

Formaldehyde is a carcinogen with highly toxic effect on organisms. ADH5 gene encodes the human formaldehyde dehydrogenase and is important to the detoxification of formaldehyde. The purpose of this work was to establish a platform to study ADH5 genetic markers induced by formaldehyde. After analyzing ADH5 SNPs database of Chinese Population, the three specific SNP sites : rs1154409,rs28730619,rs1154414 were sequenced in Human immortalized B lymphocytes of Chinese Han population by DNA sequencing. The real-time reverse transcription polymerase chain reaction (RT-PCR) approach was used to estimate expression of ADH5 mRNAs influenced by SNPs structure in human immortalized lymphocytes. Different immortalized B lymphocytes lines are gained by genotyping of multiple SNP sites of ADH5 gene. The mRNA expression of ADH5 could up regulate by 0.0005% formaldehyde for 48h exposure. But it showed rs1154409,rs28730619,rs1154414 SNPs has no association with the expression of ADH5 mRNA induced by formaldehyde. The human immortality lymphocytes model might be an efficient tool in a detection of genetic biomarkers of susceptibility to chemicals. The further research should be explored in the protein expression in more cell lines.

Sign in / Sign up

Export Citation Format

Share Document