Characterization of Ground Squirrel Retinal Ganglion Cells

1990 ◽  
Author(s):  
N. Lugo-Garcia ◽  
R. E. Blanco ◽  
Ivonne Santiago
Keyword(s):  
Retinal Ganglion Cells ◽  
Ground Squirrel ◽  
Ganglion Cells ◽  
Retinal Ganglion

scholarly journals Preliminary quantitative proteomic characterization of glaucomatous rat retinal ganglion cells

2010 ◽  
Vol 91 (1) ◽  
pp. 107-110 ◽  
Author(s):  
John W. Crabb ◽  
Xianglin Yuan ◽  
Galina Dvoriantchikova ◽  
Dmitry Ivanov ◽  
John S. Crabb ◽  
...  
Keyword(s):  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion

Characterization of Spontaneous Inhibitory Synaptic Currents in Salamander Retinal Ganglion Cells

1998 ◽  
Vol 80 (4) ◽  
pp. 1752-1764 ◽  
Author(s):  
Fan Gao ◽  
Samuel M. Wu
Keyword(s):  
Retinal Ganglion Cells ◽  
Synaptic Vesicles ◽  
Ganglion Cells ◽  
Amacrine Cells ◽  
Equilibrium Potential ◽  
Retinal Ganglion ◽  
Light Onset ◽  
Synaptic Currents ◽  
Postsynaptic Currents

Gao, Fan and Samuel M. Wu. Characterization of spontaneous inhibitory synaptic currents in salamander retinal ganglion cells. J. Neurophysiol. 80: 1752–1764, 1998. Spontaneous and light-evoked postsynaptic currents (sPSCs and lePSCs, respectively) in retinal ganglion cells of the larval tiger salamander were recorded under voltage-clamp conditions from living retinal slices. The focus of this study is to characterize the spontaneous inhibitory PSCs (sIPSCs) and their contribution to the light-evoked inhibitory PSCs (leIPSCs) in on-off ganglion cells. sIPSCs were isolated from spontaneous excitatory PSCs (sEPSCs) by application of 10 μM 6,7-dinitroquinoxaline-2,3-dione (DNQX) + 50 μM 2-amino-5-phosphonopentanoic acid (AP5). In ∼70% of on-off ganglion cells, bicuculline (or picrotoxin) completely blocks sIPSCs, suggesting all sIPSCs in these cells are mediated by GABAergic synaptic vesicles and γ-aminobutyric acid-A (GABAA) receptors (GABAergic sIPSCs, or GABAsIPSCs). In the remaining 30% of on-off ganglion cells, bicuculline (or picrotoxin) blocks 70–98% of the sIPSCs, and the remaining 2–30% are blocked by strychnine (glycinergic sIPSCs, or GLYsIPSCs). GABAsIPSCs occur randomly with an exponentially distributed interval probability density function, and they persist without noticeable rundown over time. The GABAsIPSC frequency is greatly reduced by cobalt, consistent with the idea that they are largely mediated by calcium-dependent vesicular release. GABAsIPSCs in DNQX + AP5 are tetrodotoxin (TTX) insensitive, suggesting that amacrine cells that release GABA under these conditions do not generate spontaneous action potentials. The average GABAsIPSCs exhibited linear current-voltage relation with a reversal potential near the chloride equilibrium potential, and an average peak conductance of 319.67 ± 252.83 (SD) pS. For GLYsIPSCs, the average peak conductance increase is 301.68 ± 94.34 pS. These parameters are of the same order of magnitude as those measured in inhibitory miniature postsynaptic currents (mIPSCs) associated with single synaptic vesicles in the CNS. The amplitude histograms of GABAsIPSCs did not exhibit multiple peaks, suggesting that the larger events are not discrete multiples of elementary events (or quanta). We propose that each GABAsIPSC or GLYsIPSC in retinal ganglion cells is mediated by a single or synchronized multiple of synaptic vesicles with variable neurotransmitter contents. In a sample of 16 on-off ganglion cells, the average peak leIPSC (held at 0 mV) at the light onset is 509.0 ± 233.85 pA and that at the light offset is 529.0 ± 339.88 pA. The approximate number of GABAsIPSCs and GLYsIPSCs required to generate the average light responses, calculated by the ratio of the charge (area under current traces) of the leIPSCs to that of the average single sIPSCs, is 118 ± 52 for the light onset, and 132 ± 76 for the light offset.

scholarly journals Physiological characterization of a rare subpopulation of doublet-spiking neurons in the ferret lateral geniculate nucleus

2020 ◽  
Vol 124 (2) ◽  
pp. 432-442
Author(s):  
Allison J. Murphy ◽  
J. Michael Hasse ◽  
Farran Briggs
Keyword(s):  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Visual Response ◽  
Retinal Ganglion ◽  
Visual Responses ◽  
Response Properties ◽  
Physiological Characterization ◽  
Visual Thalamus ◽  
Spike Shape

Interest in visual system homologies across species has recently increased. Across species, retinas contain diverse retinal ganglion cells including cells with unusual visual response properties. It is unclear whether rare retinal ganglion cells in carnivores project to and drive similarly unique visual responses in the visual thalamus. We discovered a rare subpopulation of thalamic neurons defined by unique spike shape and visual response properties, suggesting that nonstandard visual computations are common to many species.

Characterization of intravitreally delivered capsid mutant AAV2-Cre vector to induce tissue-specific mutations in murine retinal ganglion cells

2016 ◽  
Vol 151 ◽  
pp. 61-67
Author(s):  
Christophe J. Langouet-Astrie ◽  
Zhiyong Yang ◽  
Sraavya M. Polisetti ◽  
Derek S. Welsbie ◽  
William W. Hauswirth ◽  
...  
Keyword(s):  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Tissue Specific

scholarly journals Functional characterization of retinal ganglion cells using tailored nonlinear modeling

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Qing Shi ◽  
Pranjal Gupta ◽  
Alexandra K. Boukhvalova ◽  
Joshua H. Singer ◽  
Daniel A. Butts
Keyword(s):  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Nonlinear Modeling ◽  
Functional Characterization ◽  
Retinal Ganglion
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