Eigenfunction Methods in Magnetospheric Radial-Diffusion Theory.

1986 ◽  
Author(s):  
Michael Schulz
Keyword(s):  
Diffusion Theory ◽  
Radial Diffusion

Mass Spectrometry-Based Identification of Urinary Antimicrobial Peptides in Dairy Cows

2020 ◽  
Vol 27 (3) ◽  
pp. 225-235
Author(s):  
Ambika Sharma ◽  
Rajesh Nigam ◽  
Ashish Kumar ◽  
Simmi Singh
Keyword(s):  
Mass Spectrometry ◽  
Antimicrobial Peptides ◽  
Polyacrylamide Gel Electrophoresis ◽  
Radial Diffusion ◽  
Protein Database ◽  
Antimicrobial Effects ◽  
Cow Urine ◽  
Radial Diffusion Assay ◽  
Promising Source ◽  
Diffusion Assay

Background:: Urine is considered one of the biological fluids in which antimicrobial peptides are secreted or expressed. Cow urine has not been investigated for the presence of these peptides using MALDI-TOF-MS. Objective:: The aim of this study is to isolate, identify and assess the antimicrobial activity of urinary antimicrobial peptides from healthy normal cycling cows. Method:: We analyzed the urine sample using diafiltration, ion exchange chromatography, Reverse Phase High-Performance Liquid Chromatography (RP-HPLC), acid urea polyacrylamide gel electrophoresis (AU-PAGE) coupled with identification through Peptide Mass Fingerprinting (PMF) by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDITOF- MS). The in vitro antimicrobial effects of purified fractions were assessed using Radial Diffusion Assay (RDA) and microtitre broth dilution assay against Gram-positive and Gramnegative bacteria. Results: : Proteins corresponding to the peaks were identified using SWISSPROT protein database. This study revealed constitutive expression of β-Defensin-1 (DEFB1), β-Defensin-4A (DFB4A), Neutrophil Defensin-1 (DEF1), Neutrophil Defensin-3 (DEF3) in cow urine. The identified peptides are cationic antimicrobial peptides of the defensin family. The purified fractions exhibited antimicrobial effects in radial diffusion assay and MIC values in the range of 2.93-29.3 &*#181;M/L. Conclusion:: This study concludes that cow urine, previously unexplored with regard to antimicrobial peptides, would be a promising source of highly potent AMPs and an effective alternative to the resistant antibiotics.

The Adoption of Health Promotion Programs by State Agencies

1987 ◽  
Vol 16 (3) ◽  
pp. 235-242 ◽  
Author(s):  
Nell H. Gottlieb ◽  
Linda E. Lloyd ◽  
Jean N. Bounds
Keyword(s):  
Health Promotion ◽  
Diffusion Theory ◽  
General Interest ◽  
State Agencies ◽  
Health Promotion Programs ◽  
Potential Benefits ◽  
Education Act ◽  
One Year ◽  
Time And Energy ◽  
Personnel Directors

Personnel directors of Texas state agencies were surveyed one month prior to and one year following the passage of the 1983 Texas State Employee Health Fitness and Education Act. This legislation allowed the agencies to use available funds and facilities for health promotion programs. Most of the personnel directors were aware of the Act and of the potential benefits of health promotion programs. There was general interest in learning more about health promotion, available community resources, and about the time and energy commitments in developing a program. Most believed they would implement programs in the future. In the year following the passage of the Act, 16 percent of the agencies, covering 30,852 employees, had received approval to begin programs. Size was positively related to plan development and approval. The results are discussed using diffusion theory.

Neutron noise calculation: A comparative study between SP3 theory and diffusion theory

2021 ◽  
Vol 156 ◽  
pp. 108184
Author(s):  
Helin Gong ◽  
Zhang Chen ◽  
Wenbin Wu ◽  
Xingjie Peng ◽  
Qing Li
Keyword(s):  
Comparative Study ◽  
Diffusion Theory ◽  
Neutron Noise ◽  
And Diffusion
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