Engineering of an Extremely Thermostable Alpha/Beta Barrel Scaffold to Serve as a High Affinity Molecular Recognition Element for Use in Sensor Applications

2015 ◽  
Author(s):  
Scott Banta ◽  
Jennifer Haghpanah
Keyword(s):  
Molecular Recognition ◽  
Sensor Applications ◽  
Recognition Element ◽  
High Affinity ◽  
Beta Barrel ◽  
Alpha Beta ◽  
Molecular Recognition Element

scholarly journals In VitroSelection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ryan M. Williams ◽  
Amanda R. Kulick ◽  
Srilakshmi Yedlapalli ◽  
Louisa Battistella ◽  
Cyrus J. Hajiran ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Selection Method ◽  
Detection Methods ◽  
Sensing Element ◽  
Single Stranded Dna ◽  
Recognition Element ◽  
High Affinity ◽  
Recognition Elements ◽  
Molecular Recognition Element

Bromacil is a widely used herbicide that is known to contaminate environmental systems. Due to the hazards it presents and inefficient detection methods, it is necessary to create a rapid and efficient sensing device. Towards this end, we have utilized a stringentin vitroselection method to identify single-stranded DNA molecular recognition elements (MRE) specific for bromacil. We have identified one MRE with high affinity (Kd=9.6 nM) and specificity for bromacil compared to negative targets of selection and other pesticides. The selected ssDNA MRE will be useful as the sensing element in a field-deployable bromacil detection device.

Phage as a molecular recognition element in biosensors immobilized by physical adsorption

2007 ◽  
Vol 22 (6) ◽  
pp. 986-992 ◽  
Author(s):  
Viswaprakash Nanduri ◽  
Iryna B. Sorokulova ◽  
Alexandre M. Samoylov ◽  
Aleksandr L. Simonian ◽  
Valery A. Petrenko ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Physical Adsorption ◽  
Recognition Element ◽  
Molecular Recognition Element

Strategy for Molecular Beacon Binding Readout: Separating Molecular Recognition Element and Signal Reporter

2009 ◽  
Vol 81 (23) ◽  
pp. 9703-9709 ◽  
Author(s):  
Yongxiang Wang ◽  
Jishan Li ◽  
Jianyu Jin ◽  
Hao Wang ◽  
Hongxing Tang ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Molecular Beacon ◽  
Recognition Element ◽  
Molecular Recognition Element

scholarly journals In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine

2014 ◽  
Vol 15 (8) ◽  
pp. 14332-14347 ◽  
Author(s):  
Ryan Williams ◽  
Cassandra Crihfield ◽  
Srikanth Gattu ◽  
Lisa Holland ◽  
Letha Sooter
Keyword(s):  
Molecular Recognition ◽  
In Vitro Selection ◽  
Single Stranded Dna ◽  
Recognition Element ◽  
Molecular Recognition Element ◽  
Selection Of

Potentiometric biosensor employing catalytic antibodies as the molecular recognition element

1990 ◽  
Vol 62 (20) ◽  
pp. 2211-2216 ◽  
Author(s):  
Gary F. Blackburn ◽  
David B. Talley ◽  
Paul M. Booth ◽  
Charles N. Durfor ◽  
Mark T. Martin ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Catalytic Antibodies ◽  
Recognition Element ◽  
Molecular Recognition Element
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