Replication of Venezuelan Equine Encephalomyelitis Virus in Vitro: I. Growth in Suspension Cell Cultures Grown in Serum-Free and Defined Media

1970 ◽  
Author(s):  
Jr. Tribble ◽  
Hearn Henry R. ◽  
Nagle Henry J. ◽  
Stanley C. Jr
Keyword(s):  
Cell Cultures ◽  
Suspension Cell ◽  
Serum Free ◽  
Defined Media ◽  
Venezuelan Equine Encephalomyelitis ◽  
Encephalomyelitis Virus ◽  
Suspension Cell Cultures ◽  
Venezuelan Equine Encephalomyelitis Virus

RICKETTSIAL GROWTH IN SUSPENSION CELL CULTURES IN SERUM-FREE AND DEFINED MEDIA

1964 ◽  
Author(s):  
Jr. Tribble ◽  
Rees Henry R. ◽  
Horace B. Jr. ◽  
Jr. Hearn ◽  
Henry J.
Keyword(s):  
Cell Cultures ◽  
Suspension Cell ◽  
Serum Free ◽  
Defined Media ◽  
Suspension Cell Cultures

scholarly journals Physicochemical properties and biological activity of extracts of dried biomass of callus and suspension cells and in vitro root cultures

2020 ◽  
Vol 50 (3) ◽  
pp. 480-492
Author(s):  
Yong Yang ◽  
Lyudmila Asyakina ◽  
Olga Babich ◽  
Lyubov Dyshluk ◽  
Stanislav Sukhikh ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Cancer Cells ◽  
Cell Cultures ◽  
Biologically Active ◽  
Suspension Cell ◽  
Rhodiola Rosea ◽  
Diffusion Method ◽  
Root Cultures ◽  
Suspension Cell Cultures

Introduction. One of the urgent problems of medicine and biology is the use of plant objects as industrial producers of target metabolites in vitro. In vitro cells can be used as pharmaceutical preparations. Study objects and methods. The present research featured medicinal plants that grow in the Siberian Federal district and are a popular source of medicinal raw materials. The physicochemical properties, e.g. total ash content in extracts, the content of heavy metals, the content of organic solvents in the extracts, and the mass loss upon drying was determined by standard methods. The antimicrobial properties of in vitro extracts were determined by the diffusion method and the method based on optical density measurement. The list of opportunistic and pathogenic test strains included the following microorganisms: E. coli ATCC 25922, S. aureus ATCC 25923, P. vulgaris ATCC 63, P. aeruginosa ATCC 9027, and C. albicans EMTC 34. The number of viable cancer cells was determined using the MTT colorimetric method. Results and discussion. The paper describes the physicochemical properties, safety indicators, antioxidant activity, antimicrobial activity, and antitumor properties of extracts of a complex of biologically active substances obtained in vitro from the dried biomass of callus and suspension cell cultures and root cultures. The root extracts proved to have the maximum antimicrobial and cytotoxic properties. They could reduce the survival rate of cancer cells to 24.8–36.8 %. Conclusion. The research featured extracts obtained from the dried biomass of callus and suspension cell cultures and root cultures in vitro of safflower leuzea (Leuzea carthamoides L.), Rhodiola rosea (Rhodiola rosea L.), various sorts of skullcap (Scutellaria baicalensis L., Scutellaria andrachnoides L., Scutellaria galericulata L.), Potentilla alba (Potentilla alba L.) and ginseng (Panax L.). The results showed that the extracts can be used for the production of pharmaceuticals and biologically active additives with antitumor, antimicrobial, and antioxidant properties.

scholarly journals Replication of Venezuelan Equine Encephalomyelitis Virus In Vitro

1971 ◽  
Vol 21 (2) ◽  
pp. 342-345
Author(s):  
H. J. Hearn ◽  
H. R. Tribble ◽  
S. C. Nagle ◽  
O. C. Bowersox
Keyword(s):  
Venezuelan Equine Encephalomyelitis ◽  
Encephalomyelitis Virus ◽  
Venezuelan Equine Encephalomyelitis Virus
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