RADIOPROTECTIVE AGENTS: THE EFFECTS OF DEPOLYMERIZED YEAST RIBONUCLEIC ACID ON RADIATED HUMAN CELLS IN TISSUE CULTURE

1962 ◽  
Author(s):  
Donald E. Rounds ◽  
William C. Slick
Keyword(s):  
Tissue Culture ◽  
Ribonucleic Acid ◽  
Human Cells

scholarly journals Nuclear-Cytoplasmic Relationships in Human Cells in Tissue Culture

1959 ◽  
Vol 6 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Lester Goldstein ◽  
Julie Micou
Keyword(s):  
Tissue Culture ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Human Cells ◽  
Human Amnion ◽  
Progressive Movement ◽  
Excess Amount ◽  
In Cells

The movement of ribonucleic acid (RNA) from nucleus to cytoplasm has been studied, by autoradiographic techniques, in cells of the human amnion grown in tissue culture. Cells were exposed to cytidine-H3 for 1 hour after which time only the RNA of the nuclei was labelled. After this 1 hour exposure the cells were placed in a medium containing an excess amount of unlabelled cytidine. Periodically, cells from this medium were fixed. Autoradiographs showed that there was a progressive movement of the label from nucleus to cytoplasm, such that after 24 hours essentially all the label was in the RNA of the cytoplasm. A study of the incorporation of the cytidine-H3 in deoxyribonucleic acid (DNA), in the same population of cells at the same times, indicated that the presence of excess amounts of unlabelled cytidine almost instantaneously inhibited further utilization of cytidine-H3. It is concluded that RNA moves from nucleus to cytoplasm as a complex polynucleotide structure.

Number of nucleoli in female and male human cells in tissue culture

1962 ◽  
Vol 28 (3) ◽  
pp. 590-592 ◽  
Author(s):  
G.B. Petersen ◽  
A.J. Therkelsen
Keyword(s):  
Tissue Culture ◽  
Human Cells

Distribution of Alkaline Phosphatase Variants in a Heteroploid Strain of Human Cells in Tissue Culture

Nature ◽  
1962 ◽  
Vol 196 (4854) ◽  
pp. 600-601 ◽  
Author(s):  
JOSEPH J. MAIO ◽  
LUIGI L. DE CARLI
Keyword(s):  
Tissue Culture ◽  
Alkaline Phosphatase ◽  
Human Cells

In Vivo Effect of Heparin on Acid and Alkaline Ribonuclease Activities of Mouse Liver

1956 ◽  
Vol 184 (2) ◽  
pp. 415-417 ◽  
Author(s):  
Gaston De Lamirande ◽  
George Weber ◽  
Antonio Cantero
Keyword(s):  
Tissue Culture ◽  
Body Weight ◽  
Single Dose ◽  
Ribonucleic Acid ◽  
Mouse Liver ◽  
Single Injection ◽  
Coagulation Time ◽  
Alkaline Ribonuclease ◽  
Blood Coagulability

A single dose of 30 µg/gm body weight of depo-heparin was injected subcutaneously into white Swiss mice. At 1, 3, 6 and 12 hours after the injection, the blood coagulation time was measured and the activity of acid and alkaline ribonuclease of liver was determined. This single injection of depo-heparin significantly inhibited the acid and alkaline ribonucleases of liver 1 hour after injection. The enzymatic activities significantly increased after the blood coagulability was restored. The in vivo inhibition of acid and alkaline ribonuclease activity supports the explanation that the accumulation of ribonucleic acid in cells of tissue culture in the presence of heparin might be due to the inhibition of ribonuclease.

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