Fish play a sizable role in the remains of ancient Greek comedy. Although scholars have proposed various cultural, economic, and generic explanations for comedy’s interest in sea creatures, they have not adequately considered the importance of seafood’s relationship to obscenity and sexuality. Greek comic poets correlate a range of sea creatures with sex and sexuality in imaginative and humorous ways, making obscene jokes about courtesans and aphrodisiacs, as well as creating double entendres for male and female genitalia. This study provides a lexical resource for Greek comedy’s numerous seafood fragments, uncovering many neglected ancient sexual jokes and offering fresh insight on comedy’s interest in sea creatures.