scholarly journals Overexpression of GmNFYA5 confers drought tolerance to transgenic Arabidopsis and soybean plants

2020 ◽  
Author(s):  
Xiao-Jun Ma ◽  
Tai-Fei Yu ◽  
Xiao-Hui Li ◽  
Xin-You Cao ◽  
Jian Ma ◽  
...  
Keyword(s):  
Glycine Max ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Transcript Level ◽  
Transcript Abundance ◽  
Crop Productivity ◽  
Growth Stages ◽  
Synthesis Inhibitor

Abstract Background: Crop productivity is challenged by abiotic stresses, among which drought stress is the most common. NF-Y genes, especially NF-YA genes, regulate tolerance to abiotic stress. Results: Soybean NF-Y gene GmNFYA5 was identified to have the highest transcript level among all 21 NF-YA genes in soybean ( Glycine max L.) under drought stress. Drought-induced transcript of GmNFYA5 was suppressed by the ABA synthesis inhibitor naproxen (NAP). GmNFYA5 transcript was detected in various tissues at vegetative and reproductive growth stages with higher levels in roots and leaves than in other tissues, which was consist with the GmNFYA5 promoter:GUS fusion assay. Overexpression of GmNFYA5 in transgenic Arabidopsis plants caused enhanced drought tolerance in seedlings by decreasing stomatal aperture and water loss from leaves. Overexpression and suppression of GmNFYA5 in soybean resulted in increased and decreased drought tolerance, respectively, relative to plants with an empty vector (EV). Transcript levels of ABA-dependent genes ( ABI2 , ABI3 , NCED3 , LEA3 , RD29A , P5CS1 , GmWRKY46 , GmNCED2 and GmbZIP1 ) and ABA-independent genes ( DREB1A , DREB2A , DREB2B , GmDREB1 , GmDREB2 and GmDREB3 ) in transgenic plants overexpressing GmNFYA5 were higher than those of wild-type plants under drought stress; suppression of GmNFYA5 transcript produced opposite results. GmNFYA5 probably regulated the transcript abundance of GmDREB2 and GmbZIP1 by binding to the promoters in vivo. Conclusions: Our results suggested that overexpression of GmNFYA5 improved drought tolerance in soybean via both ABA-dependent and ABA-independent pathways. Keywords: ABA sensitivity, Glycine max , Nuclear Factor YA, resistance mechanisms

scholarly journals Overexpression of GmNFYA5 confers drought tolerance to transgenic Arabidopsis and soybean plants

2020 ◽  
Author(s):  
Xiao-Jun Ma ◽  
Tai-Fei Yu ◽  
Xiao-Hui Li ◽  
Xin-You Cao ◽  
Jian Ma ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Transcript Level ◽  
Transcript Abundance ◽  
Crop Productivity ◽  
Growth Stages ◽  
Synthesis Inhibitor ◽  
Vegetative And Reproductive Growth

Abstract Background: Crop productivity is challenged by abiotic stresses, among which drought stress is the most common. NF-Y genes, especially NF-YA genes, regulate tolerance to abiotic stress. Results: Soybean NF-Y gene GmNFYA5 was identified to have the highest transcript level among all 21 NF-YA genes in soybean ( Glycine max L.) under drought stress. Drought-induced transcript of GmNFYA5 was suppressed by the ABA synthesis inhibitor naproxen (NAP). GmNFYA5 transcript was detected in various tissues at vegetative and reproductive growth stages with higher levels in roots and leaves than in other tissues, which was consist with the GmNFYA5 promoter:GUS fusion assay. Overexpression of GmNFYA5 in transgenic Arabidopsis plants caused enhanced drought tolerance in seedlings by decreasing stomatal aperture and water loss from leaves. Overexpression and suppression of GmNFYA5 in soybean resulted in increased and decreased drought tolerance, respectively, relative to plants with an empty vector (EV). Transcript levels of ABA-dependent genes ( ABI2 , ABI3 , NCED3 , LEA3 , RD29A , P5CS1 , GmWRKY46 , GmNCED2 and GmbZIP1 ) and ABA-independent genes ( DREB1A , DREB2A , DREB2B , GmDREB1 , GmDREB2 and GmDREB3 ) in transgenic plants overexpressing GmNFYA5 were higher than those of wild-type plants under drought stress; suppression of GmNFYA5 transcript produced opposite results. GmNFYA5 probably regulated the transcript abundance of GmDREB2 and GmbZIP1 by binding to the promoters in vivo. Conclusions: Our results suggested that overexpression of GmNFYA5 improved drought tolerance in soybean via both ABA-dependent and ABA-independent pathways.

scholarly journals Overexpression of GmNFYA5 confers drought tolerance to transgenic Arabidopsis and soybean plants

2020 ◽  
Author(s):  
Xiao-Jun Ma ◽  
Tai-Fei Yu ◽  
Xiao-Hui Li ◽  
Xin-You Cao ◽  
Jian Ma ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Transcript Level ◽  
Transcript Abundance ◽  
Crop Productivity ◽  
Growth Stages ◽  
Synthesis Inhibitor ◽  
Vegetative And Reproductive Growth

Abstract Background: Crop productivity is challenged by abiotic stresses, among which drought stress is the most common. NF-Y genes, especially NF-YA genes, regulate tolerance to abiotic stress. Results: Soybean NF-Y gene GmNFYA5 was identified to have the highest transcript level among all 21 NF-YA genes in soybean (Glycine max L.) under drought stress. Drought-induced transcript of GmNFYA5 was suppressed by the ABA synthesis inhibitor naproxen (NAP). GmNFYA5 transcript was detected in various tissues at vegetative and reproductive growth stages with higher levels in roots and leaves than in other tissues, which was consist with the GmNFYA5 promoter:GUS fusion assay. Overexpression of GmNFYA5 in transgenic Arabidopsis plants caused enhanced drought tolerance in seedlings by decreasing stomatal aperture and water loss from leaves. Overexpression and suppression of GmNFYA5 in soybean resulted in increased and decreased drought tolerance, respectively, relative to plants with an empty vector (EV). Transcript levels of ABA-dependent genes (ABI2, ABI3, NCED3, LEA3, RD29A, P5CS1, GmWRKY46, GmNCED2 and GmbZIP1) and ABA-independent genes (DREB1A, DREB2A, DREB2B, GmDREB1, GmDREB2 and GmDREB3) in transgenic plants overexpressing GmNFYA5 were higher than those of wild-type plants under drought stress; suppression of GmNFYA5 transcript produced opposite results. GmNFYA5 probably regulated the transcript abundance of GmDREB2 and GmbZIP1 by binding to the promoters in vivo.Conclusions: Our results suggested that overexpression of GmNFYA5 improved drought tolerance in soybean via both ABA-dependent and ABA-independent pathways.

scholarly journals Overexpression of GmNFYA5 confers drought tolerance to transgenic Arabidopsis and soybean plants

2019 ◽  
Author(s):  
Xiao-Jun Ma ◽  
Tai-Fei Yu ◽  
Xiao-Hui Li ◽  
Xin-You Cao ◽  
Jian Ma ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Transcript Level ◽  
Transcript Abundance ◽  
Crop Productivity ◽  
Growth Stages ◽  
Synthesis Inhibitor ◽  
Vegetative And Reproductive Growth

Abstract Background: Crop productivity is challenged by abiotic stresses, among which drought stress is the most common. NF-Y genes, especially NF-YA genes, regulate tolerance to abiotic stress. Results: Soybean NF-Y gene GmNFYA5 was identified to have the highest transcript level among all 21 NF-YA genes in soybean ( Glycine max L.) under drought stress. Drought-induced transcript of GmNFYA5 was suppressed by the ABA synthesis inhibitor naproxen (NAP). GmNFYA5 transcript was detected in various tissues at vegetative and reproductive growth stages with higher levels in roots and leaves than in other tissues, which was consist with the GmNFYA5 promoter:GUS fusion assay. Overexpression of GmNFYA5 in transgenic Arabidopsis plants caused enhanced drought tolerance in seedlings by decreasing stomatal aperture and water loss from leaves. Overexpression and suppression of GmNFYA5 in soybean resulted in increased and decreased drought tolerance, respectively, relative to plants with an empty vector (EV). Transcript levels of ABA-dependent genes ( ABI2 , ABI3 , NCED3 , LEA3 , RD29A , P5CS1 , GmWRKY46 , GmNCED2 and GmbZIP1 ) and ABA-independent genes ( DREB1A , DREB2A , DREB2B , GmDREB1 , GmDREB2 and GmDREB3 ) in transgenic plants overexpressing GmNFYA5 were higher than those of wild-type plants under drought stress; suppression of GmNFYA5 transcript produced opposite results. GmNFYA5 probably regulated the transcript abundance of GmDREB2 and GmbZIP1 by binding to the promoters in vivo. Conclusions: Our results suggested that overexpression of GmNFYA5 improved drought tolerance in soybean via both ABA-dependent and ABA-independent pathways.

scholarly journals Overexpression of GmNFYA5 confers drought tolerance to transgenic Arabidopsis and soybean plants

2019 ◽  
Author(s):  
Xiao-Jun Ma ◽  
Tai-Fei Yu ◽  
Xiao-Hui Li ◽  
Xin-You Cao ◽  
Jian Ma ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Transcript Level ◽  
Crop Productivity ◽  
Synthesis Inhibitor ◽  
Glycine Max L ◽  
Soybean Plants ◽  
Vegetative And Reproductive Growth

Abstract Background: The crop productivity is challenged by abiotic stresses, among which drought stress is the most widespread . The NF-Y genes, especially NF-YA genes function in regulating drought tolerance of plants. Results: In this study, a soybean NF-Y gene, GmNFYA5 , was identified with the highest transcript level among all of 21 NF-YA genes in soybean ( Glycine max L.) under drought stress. Transcript of GmNFYA5 induced by drought was suppressed by ABA synthesis inhibitor naproxen (NAP). GmNFYA5 transcript was detected in various tissues at vegetative and reproductive growth stage with higher levels in roots and leaves, which was consist with the GmNFYA5 promoter:GUS fusion assay. Overexpression of GmNFYA5 resulted in increased drought tolerance and ABA sensitivity in transgenic Arabidopsis plants. Additionally, overexpression and suppression of GmNFYA5 in soybean resulted in increased and decreased drought tolerance compared with empty vector (EV) plants respectively. Transcript levels of ABA-dependent and ABA-independent genes in transgenic Arabidopsis and soybean plants overexpressing GmNFYA5 were higher than those of WT and EV plants under drought stress respectively, but the opposite results were detected in soybean plants suppressing GmNFYA5 . Furthermore, GmNFYA5 might regulate the expression abundance of GmDREB2 and GmbZIP1 by binding the promoter in vivo. Conclusions: These results suggest that overexpression of GmNFYA5 can improve drought tolerance via ABA-dependent and ABA-independent pathways in soybean.

Morphological and Physiological Performance of Indian Soybean [Glycine max (L.) Merrill] Genotypes in Respect to Drought 

2021 ◽  
Author(s):  
Nishi Mishra ◽  
M.K. Tripathi ◽  
Sushma Tiwari ◽  
Niraj Tripathi ◽  
Neha Gupta ◽  
...  
Keyword(s):  
Glycine Max ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Metabolic Activity ◽  
Growth Stages ◽  
Drought Tolerant ◽  
Soybean Genotypes ◽  
Physiological Analysis ◽  
Glycine Max L ◽  
Different Growth Stages

Background: Soybean is a key crop that grants an imperative supply of oils and proteins to humans and animals; however, its productivity spectacularly diminished owing to the occurrence of drought stress. Methods: The present investigation was executed during Kharif 2018-2019 to recognize drought tolerant genotypes on the basis of an array of morpho-physiological traits. Morpho-physiological analysis among 53 genotypes divulged the existence of drought tolerance capability in studied genotypes.Result: On the basis of current findings, it can be concluded that drought stress retards the growth and metabolic activity of soybean genotypes. These parameters showed considerable amount of variability under drought stress at different growth stages in soybean. Among 53 soybean genotypes, four genotypes viz., JS97-52, AMS 2014-1, RVS-14 and NRC-147 was found to be drought tolerant.

scholarly journals CsCYT75B1, a Citrus CYTOCHROME P450 Gene, Is Involved in Accumulation of Antioxidant Flavonoids and Induces Drought Tolerance in Transgenic Arabidopsis

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 161 ◽  
Author(s):  
Muhammad Junaid Rao ◽  
Yuantao Xu ◽  
Xiaomei Tang ◽  
Yue Huang ◽  
Jihong Liu ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Wild Type ◽  
Ros Scavenging ◽  
Cytochrome P450 Gene ◽  
P450 Gene ◽  
Large Gene ◽  
Transgenic Lines

CYTOCHROME P450s genes are a large gene family in the plant kingdom. Our earlier transcriptome data revealed that a CYTOCHROME P450 gene of Citrus sinensis (CsCYT75B1) was associated with flavonoid metabolism and was highly induced after drought stress. Here, we characterized the function of CsCYT75B1 in drought tolerance by overexpressing it in Arabidopsis thaliana. Our results demonstrated that the overexpression of the CsCYT75B1 gene significantly enhanced the total flavonoid contents with increased antioxidant activity in transgenic Arabidopsis. The gene expression results showed that several genes that are responsible for the biosynthesis of antioxidant flavonoids were induced by 2–12 fold in transgenic Arabidopsis lines. After 14 days of drought stress, all transgenic lines displayed an enhanced tolerance to drought stress along with accumulating antioxidant flavonoids with lower superoxide radicals and reactive oxygen species (ROS) than wild type plants. In addition, drought-stressed transgenic lines possessed higher antioxidant enzymatic activities than wild type transgenic lines. Moreover, the stressed transgenic lines had significantly lower levels of electrolytic leakage than wild type transgenic lines. These results demonstrate that the CsCYT75B1 gene of sweet orange functions in the metabolism of antioxidant flavonoid and contributes to drought tolerance by elevating ROS scavenging activities.

scholarly journals Seed Priming: A Feasible Strategy to Enhance Drought Tolerance in Crop Plants

2020 ◽  
Vol 21 (21) ◽  
pp. 8258 ◽  
Author(s):  
Vishvanathan Marthandan ◽  
Rathnavel Geetha ◽  
Karunanandham Kumutha ◽  
Vellaichamy Gandhimeyyan Renganathan ◽  
Adhimoolam Karthikeyan ◽  
...  
Keyword(s):  
Drought Stress ◽  
Seed Germination ◽  
Drought Tolerance ◽  
Defense Mechanism ◽  
Seed Priming ◽  
Crop Productivity ◽  
Crop Plants ◽  
Complex Nature ◽  
Efficient Manner ◽  
Farming Community

Drought is a serious threat to the farming community, biasing the crop productivity in arid and semi-arid regions of the world. Drought adversely affects seed germination, plant growth, and development via non-normal physiological processes. Plants generally acclimatize to drought stress through various tolerance mechanisms, but the changes in global climate and modern agricultural systems have further worsened the crop productivity. In order to increase the production and productivity, several strategies such as the breeding of tolerant varieties and exogenous application of growth regulators, osmoprotectants, and plant mineral nutrients are followed to mitigate the effects of drought stress. Nevertheless, the complex nature of drought stress makes these strategies ineffective in benefiting the farming community. Seed priming is an alternative, low-cost, and feasible technique, which can improve drought stress tolerance through enhanced and advanced seed germination. Primed seeds can retain the memory of previous stress and enable protection against oxidative stress through earlier activation of the cellular defense mechanism, reduced imbibition time, upsurge of germination promoters, and osmotic regulation. However, a better understanding of the metabolic events during the priming treatment is needed to use this technology in a more efficient way. Interestingly, the review highlights the morphological, physiological, biochemical, and molecular responses of seed priming for enhancing the drought tolerance in crop plants. Furthermore, the challenges and opportunities associated with various priming methods are also addressed side-by-side to enable the use of this simple and cost-efficient technique in a more efficient manner.

scholarly journals The interplay between miR156/SPL13 and DFR/WD40–1 regulate drought tolerance in alfalfa

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Biruk A. Feyissa ◽  
Muhammad Arshad ◽  
Margaret Y. Gruber ◽  
Susanne E. Kohalmi ◽  
Abdelali Hannoufa
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Anthocyanin Biosynthesis ◽  
Physiological Responses ◽  
Biological Functions ◽  
Altered Expression ◽  
Transcript Levels ◽  
Squamosa Promoter Binding Protein ◽  
Fine Tune

Abstract Background Developing Medicago sativa L. (alfalfa) cultivars tolerant to drought is critical for the crop’s sustainable production. miR156 regulates various plant biological functions by silencing SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors. Results To understand the mechanism of miR156-modulated drought stress tolerance in alfalfa we used genotypes with altered expression levels of miR156, miR156-regulated SPL13, and DIHYDROFLAVONOL-4-REDUCTASE (DFR) regulating WD40–1. Previously we reported the involvement of miR156 in drought tolerance, but the mechanism and downstream genes involved in this process were not fully studied. Here we illustrate the interplay between miR156/SPL13 and WD40–1/DFR to regulate drought stress by coordinating gene expression with metabolite and physiological strategies. Low to moderate levels of miR156 overexpression suppressed SPL13 and increased WD40–1 to fine-tune DFR expression for enhanced anthocyanin biosynthesis. This, in combination with other accumulated stress mitigating metabolites and physiological responses, improved drought tolerance. We also demonstrated that SPL13 binds in vivo to the DFR promoter to regulate its expression. Conclusions Taken together, our results reveal that moderate relative miR156 transcript levels are sufficient to enhance drought resilience in alfalfa by silencing SPL13 and increasing WD40–1 expression, whereas higher miR156 overexpression results in drought susceptibility.

scholarly journals Direct Infusion Metabolomics of the Photosystem and Chlorophyll Related Metabolites within a Drought Tolerant Plant Introduction of Glycine max

Metabolites ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 843
Author(s):  
Kevin J. Zemaitis ◽  
Heng Ye ◽  
Henry T. Nguyen ◽  
Troy D. Wood
Keyword(s):  
Mass Spectrometry ◽  
Glycine Max ◽  
Drought Stress ◽  
Plant Introduction ◽  
Field Trials ◽  
Growth Stages ◽  
Ion Cyclotron Resonance ◽  
Direct Infusion ◽  
Phytochemical Content ◽  
Chl A

Drought is the most prolific form of abiotic stress that legumes and cereal plants alike can endure, and the planting of an improper cultivar at the beginning of a season can cause unexpected losses up to fifty percent under water deficient conditions. Herein, a plant introduction (PI) of an exotic cultivar of soybean (Glycine max), PI 567731, which demonstrates a slow wilting (SW) canopy phenotype in maturity group III, was profiled under drought conditions in field trials in Missouri against a drought susceptible check cultivar, Pana. Metabolomic profiling was carried out on samples of leaves from each of these cultivars at V5 and R2 growth stages both while irrigated and while under drought stress for three weeks. PI 567731 was observed to have differential phytochemical content, and enhanced levels of chlorophyll (Chl) a/b and pheophytin (Pheo) were profiled by direct infusion electrospray Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Indicating drought induced changes of the photosystem and photosynthetic capabilities alongside water preservation strategies are important within the SW phenotype drought response. Subsequent multivariate analysis was able to form predictive models, encompassing the variance of growth and drought stress of the cultivar. Moreover, the existence of unique Chl-related metabolites (CRM) (m/z > 900) were confirmed through tandem mass spectrometry. The resultant coordination of fatty acids to the core of the porphyrin ring was observed and played an unknown role in the proliferation of the photosynthesis. However, the relative ratio of the most abundant CRM is undisturbed by drought stress in PI 567731, in contrast to the drought susceptible cultivar. These results provide key insights into drought related metabolic mechanisms.

scholarly journals VlbZIP30 of grapevine functions in drought tolerance via the abscisic acid core signaling pathway

2017 ◽  
Author(s):  
Mingxing Tu ◽  
Xianhang Wang ◽  
Yanxun Zhu ◽  
Dejun Wang ◽  
Xuechuan Zhang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Signaling Pathway ◽  
Growth And Development ◽  
Transgenic Arabidopsis ◽  
Dehydration Stress ◽  
Aba Signaling ◽  
Group A ◽  
Cotyledon Greening

AbstractDrought stress limits the growth and development of grapevines, thereby reducing productivity, but the mechanisms by which grapevines respond to drought stress remain largely uncharacterized. Here, we characterized a group A bZIP gene from ‘Kyoho’ grapevine, VlbZIP30, which was shown to be induced by abscisic acid (ABA) and dehydration stress. Overexpression of VlbZIP30 in transgenic Arabidopsis enhanced dehydration tolerance during seed germination, and in the seedling and adult stages. Transcriptome analysis revealed that a major proportion of ABA- and/or drought-responsive genes are transcriptionally regulated by VlbZIP30 during ABA or mannitol treatment at the cotyledon greening stage. We identified an A. thaliana G-box motif (CACGTG) and a potential grapevine G-box motif (MCACGTGK) in the promoters of the 39 selected A. thaliana genes up-regulated in the transgenic plants and in the 35 grapevine homologs, respectively. Subsequently, using two grapevine-related databases, we found that 74% and 84% (a total of 27 genes) of the detected grapevine genes were significantly up-regulated by ABA and drought stress, respectively, suggesting that these 27 genes involve in ABA or dehydration stress and may be regulated by VlbZIP30 in grapevine. We propose that VlbZIP30 functions as a positive regulator of drought-responsive signaling in the ABA core signaling pathway.HighlightVlbZIP30 positively regulate plant drought tolerance through regulated the expression of 27 grapevine candidate genes via G-box cis-element (MCACGTGK) in ABA signaling pathway.

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