Step-Up Concentration Method for Chemical Agents’ Adsorption Measurement in Porous Media

2019 ◽  
Author(s):  
Vasilii Lompik ◽  
Nikita Dadakin ◽  
Marat Nukhaev ◽  
Konstantin Rymarenko ◽  
Artem Makatrov ◽  
...  
Keyword(s):  
Porous Media ◽  
Concentration Method ◽  
Chemical Agents ◽  
Adsorption Measurement

Step-Up Concentration Method for Chemical Agents’ Adsorption Measurement in Porous Media (Russian)

2019 ◽  
Author(s):  
Vasilii Lompik ◽  
Nikita Dadakin ◽  
Marat Nukhaev ◽  
Konstantin Rymarenko ◽  
Artem Makatrov ◽  
...  
Keyword(s):  
Porous Media ◽  
Concentration Method ◽  
Chemical Agents ◽  
Adsorption Measurement

Effect of Acetone and Kerosene on Skin Ultrastructure

1972 ◽  
Vol 30 ◽  
pp. 92-93
Author(s):  
A. P. Lupulescu ◽  
H. Pinkus ◽  
D. J. Birmingham
Keyword(s):  
Electron Microscopy ◽  
Human Skin ◽  
Osmium Tetroxide ◽  
Human Epidermis ◽  
Ultrastructural Changes ◽  
Chemical Agents ◽  
Skin Ultrastructure ◽  
Volar Surface

Our laboratory is engaged in the study of the effect of different chemical agents on human skin, using electron microscopy. Previous investigations revealed that topical use of a strong alkali (NaOH 1N) or acid (HCl 1N), induces ultrastructural changes in the upper layers of human epidermis. In the current experiments, acetone and kerosene, which are primarily lipid solvents, were topically used on the volar surface of the forearm of Caucasian and Negro volunteers. Skin specimens were bioptically removed after 90 min. exposure and 72. hours later, fixed in 3% buffered glutaraldehyde, postfixed in 1% phosphate osmium tetroxide, then flat embedded in Epon.

Cytochemical Specializations on the Luminal Surface of Blood Vessels in the Developing Rat Central Nervous System

1975 ◽  
Vol 33 ◽  
pp. 462-463
Author(s):  
R. S. Hannah ◽  
T. H. Rosenquist
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Blood Vessels ◽  
Alcian Blue ◽  
Luminal Surface ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron ◽  
Concentration Method ◽  
Endothelial Surface ◽  
Cacodylate Buffer

Developing blood vessels in the rat central nervous system exhibit several unusual luminal features. Hannah (1975) used high voltage electron microscopy to demonstrate numerous ridges of endothelium, some near junctional complexes. The ridges produced troughs (which may appear as depressions) in the endothelial surface. In some areas ridges extended over the troughs, removing them from direct contact with the luminal surface. At no time were the troughs observed to penetrate the basal laminae. Fingerlike projections also extended into the lumina.To determine whether any chemical specializations accompanied the unusual morphological features of the luminal surface, we added 0.1% Alcian blue (Behnke and Zelander, 1970) to the 3% glutaraldehyde perfusate (cacodylate buffer, pH 7.4). After Alcian blue had reacted with the luminal glycocalyces, the dye was dissociated with MgCl2 via critical electrolyte concentration method of Scott and Dorling (1965). When these methods are applied together, it is possible to differentiate mucopolysaccharides (glycosaminoglycans or GAG) with the electron microscope.

Sign in / Sign up

Export Citation Format

Share Document