Potential Application of Crude Oil Degrading Bacteria in Oil Spill and Waste Management

2018 ◽  
Author(s):  
M. Al Mujaini ◽  
S. J. Joshi ◽  
N. Sivakumar ◽  
S. N. Al-Bahry
Keyword(s):  
Waste Management ◽  
Crude Oil ◽  
Oil Spill ◽  
Potential Application ◽  
Degrading Bacteria

scholarly journals Oceanobacter-related bacteria are important for the degradation of petroleum aliphatic hydrocarbons in the tropical marine environment

Microbiology ◽  
2009 ◽  
Vol 155 (10) ◽  
pp. 3362-3370 ◽  
Author(s):  
Maki Teramoto ◽  
Masahito Suzuki ◽  
Fumiyoshi Okazaki ◽  
Ariani Hatmanti ◽  
Shigeaki Harayama
Keyword(s):  
Crude Oil ◽  
Oil Spill ◽  
Marine Environment ◽  
Petroleum Hydrocarbon ◽  
Rrna Gene ◽  
Alkane Degradation ◽  
Degrading Bacteria ◽  
Tropical Sea ◽  
Nitrogen Phosphorus ◽  
Crude Oil Spill

Petroleum-hydrocarbon-degrading bacteria were obtained after enrichment on crude oil (as a ‘chocolate mousse’) in a continuous supply of Indonesian seawater amended with nitrogen, phosphorus and iron nutrients. They were related to Alcanivorax and Marinobacter strains, which are ubiquitous petroleum-hydrocarbon-degrading bacteria in marine environments, and to Oceanobacter kriegii (96.4–96.5 % similarities in almost full-length 16S rRNA gene sequences). The Oceanobacter-related bacteria showed high n-alkane-degrading activity, comparable to that of Alcanivorax borkumensis strain SK2. On the other hand, Alcanivorax strains exhibited high activity for branched-alkane degradation and thus could be key bacteria for branched-alkane biodegradation in tropical seas. Oceanobacter-related bacteria became most dominant in microcosms that simulated a crude oil spill event with Indonesian seawater. The dominance was observed in microcosms that were unamended or amended with fertilizer, suggesting that the Oceanobacter-related strains could become dominant in the natural tropical marine environment after an accidental oil spill, and would continue to dominate in the environment after biostimulation. These results suggest that Oceanobacter-related bacteria could be major degraders of petroleum n-alkanes spilt in the tropical sea.

scholarly journals Photocatalytic degradation of fractionated crude oil: potential application in oil spill remediation

2020 ◽  
Vol 7 (1) ◽  
pp. 1744944 ◽  
Author(s):  
Benjamin Agyei-Tuffour ◽  
Selassie Gbogbo ◽  
David Dodoo-Arhin ◽  
Lucas N.W. Damoah ◽  
Johnson K. Efavi ◽  
...  
Keyword(s):  
Crude Oil ◽  
Photocatalytic Degradation ◽  
Oil Spill ◽  
Potential Application ◽  
Oil Spill Remediation

scholarly journals Hydrocarbon-Degrading Bacteria Exhibit a Species-Specific Response to Dispersed Oil while Moderating Ecotoxicity

2015 ◽  
Vol 82 (2) ◽  
pp. 518-527 ◽  
Author(s):  
Will A. Overholt ◽  
Kala P. Marks ◽  
Isabel C. Romero ◽  
David J. Hollander ◽  
Terry W. Snell ◽  
...  
Keyword(s):  
Crude Oil ◽  
Oil Spill ◽  
Environmental Protection Agency ◽  
Synergistic Effects ◽  
Bacterial Species ◽  
Specific Response ◽  
Oil Degradation ◽  
Content Type ◽  
Dispersed Oil ◽  
Degrading Bacteria

ABSTRACTThe Deepwater Horizon blowout in April 2010 represented the largest accidental marine oil spill and the largest release of chemical dispersants into the environment to date. While dispersant application may provide numerous benefits to oil spill response efforts, the impacts of dispersants and potential synergistic effects with crude oil on individual hydrocarbon-degrading bacteria are poorly understood. In this study, two environmentally relevant species of hydrocarbon-degrading bacteria were utilized to quantify the response to Macondo crude oil and Corexit 9500A-dispersed oil in terms of bacterial growth and oil degradation potential. In addition, specific hydrocarbon compounds were quantified in the dissolved phase of the medium and linked to ecotoxicity using a U.S. Environmental Protection Agency (EPA)-approved rotifer assay. Bacterial treatment significantly and drastically reduced the toxicity associated with dispersed oil (increasing the 50% lethal concentration [LC50] by 215%). The growth and crude oil degradation potential ofAcinetobacterwere inhibited by Corexit by 34% and 40%, respectively; conversely, Corexit significantly enhanced the growth ofAlcanivoraxby 10% relative to that in undispersed oil. Furthermore, both bacterial strains were shown to grow with Corexit as the sole carbon and energy source. Hydrocarbon-degrading bacterial species demonstrate a unique response to dispersed oil compared to their response to crude oil, with potentially opposing effects on toxicity. While some species have the potential to enhance the toxicity of crude oil by producing biosurfactants, the same bacteria may reduce the toxicity associated with dispersed oil through degradation or sequestration.

MICROBIAL COMMUNITY DURING BIOREMEDIATION EXPERIMENTAL ON OIL SPILL IN COASTAL OF PARI ISLAND

2011 ◽  
Vol 3 (1) ◽  
Author(s):  
Lies Indah Sutiknowati
Keyword(s):  
Oil Spill ◽  
Deoxyribonucleic Acid ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Degradation ◽  
Sequence Determination ◽  
Blast Search ◽  
Degrading Bacteria ◽  
Gradient Gel Electrophoresis

There is an information how to identify hydrocarbon degrading bacteria for bioremediation of marine oil spill. We have Bioremediation treatment for degradation of oil spill on Pari island and need two kind of experiment there are tanks experiment (sampling 0 to 90 days) and semi enclosed system (sampling 0 to 150 days). Biostimulation with nutrients (N and P) was done to analyze biodegradation of hydrocarbon compounds. Experiment design using fertilizer Super IB and Linstar will stimulate bacteria can degrade oil, n-alkane, and alkane as poly aromatic hydrocarbon. The bacteria communities were monitored and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE) and Clone Library; oil chemistry was analyzed by Gas Chromatography Mass Spectrometry (GCMS). DNA (deoxyribonucleic acid) was extracted from colonies of bacteria and sequence determination of the 16S rDNA was amplified by primers U515f and U1492r. Strains had been sequence and had similarity about 90-99% to their closest taxa by homology Blast search and few of them suspected as new species. The results showed that fertilizers gave a significant effect on alkane, PAH and oil degradation in tanks experiment but not in the field test. Dominant of the specific bacteria on this experiment were Alcanivorax, Marinobacter and Prosthecochloris. Keywords: Bioremediation, Biostimulation, DGGE, PAH, Pari Island

scholarly journals Effects of Dispersants and Biosurfactants on Crude-Oil Biodegradation and Bacterial Community Succession

2021 ◽  
Vol 9 (6) ◽  
pp. 1200
Author(s):  
Gareth E. Thomas ◽  
Jan L. Brant ◽  
Pablo Campo ◽  
Dave R. Clark ◽  
Frederic Coulon ◽  
...  
Keyword(s):  
Crude Oil ◽  
Early Stage ◽  
Niche Partitioning ◽  
Hydrocarbon Biodegradation ◽  
Hydrocarbonoclastic Bacteria ◽  
Bacterial Response ◽  
Oil Biodegradation ◽  
Interfacial Surface ◽  
Degrading Bacteria ◽  
Oil Spill Response

This study evaluated the effects of three commercial dispersants (Finasol OSR 52, Slickgone NS, Superdispersant 25) and three biosurfactants (rhamnolipid, trehalolipid, sophorolipid) in crude-oil seawater microcosms. We analysed the crucial early bacterial response (1 and 3 days). In contrast, most analyses miss this key period and instead focus on later time points after oil and dispersant addition. By focusing on the early stage, we show that dispersants and biosurfactants, which reduce the interfacial surface tension of oil and water, significantly increase the abundance of hydrocarbon-degrading bacteria, and the rate of hydrocarbon biodegradation, within 24 h. A succession of obligate hydrocarbonoclastic bacteria (OHCB), driven by metabolite niche partitioning, is demonstrated. Importantly, this succession has revealed how the OHCB Oleispira, hitherto considered to be a psychrophile, can dominate in the early stages of oil-spill response (1 and 3 days), outcompeting all other OHCB, at the relatively high temperature of 16 °C. Additionally, we demonstrate how some dispersants or biosurfactants can select for specific bacterial genera, especially the biosurfactant rhamnolipid, which appears to provide an advantageous compatibility with Pseudomonas, a genus in which some species synthesize rhamnolipid in the presence of hydrocarbons.

EFFORT TO RESCUE OTTERS AFTER SPILL IS IN DISPUTE

PEDIATRICS ◽  
1991 ◽  
Vol 88 (3) ◽  
pp. 661-661
Author(s):  
J. F. L.
Keyword(s):  
Crude Oil ◽  
Oil Spill ◽  
Preliminary Data ◽  
Scientific Information ◽  
Research Projects ◽  
Exxon Valdez ◽  
Sea Otters ◽  
Exxon Valdez Oil Spill ◽  
Rehabilitation Centers ◽  
Government Contract

ANCHORAGE, April 20—Sea otters rescued from waters fouled by the Exxon Valdez oil spill fared poorly after their return to the wild last fall, and scientists working under Government contract say at least half may have perished over the winter. Nearly 900 dead otters were found after the tanker spilled almost 11 million gallons of crude oil in March 1989. An additional 360 were netted alive and brought to rehabilitation centers at Valdez, Seward, Homer and Kodiak. About 200 were later returned to Prince William Sound but some scientists say that as many as half may have perished and that the rehabilitation effort has been largely futile... The withholding of scientific information on the spill for legal reasons by all the parties is becoming a major source of controversy as research projects begin to generate at least preliminary data.

scholarly journals CRUDE OIL BIOREMEDIATION BY INDIGENOUS BACTERIA ISOLATED FROM OILY SLUDGE

2016 ◽  
Vol 78 (11-2) ◽  
Author(s):  
Nur Hafizah Azizan ◽  
Kasing Ak Apun ◽  
Lesley Maurice Bilung ◽  
Micky Vincent ◽  
Hairul Azman Roslan ◽  
...  
Keyword(s):  
Crude Oil ◽  
Enrichment Culture ◽  
Molecular Techniques ◽  
Oily Sludge ◽  
Minimal Salt Medium ◽  
Alkane Degradation ◽  
Growth Study ◽  
The Third ◽  
Cell Counts ◽  
Degrading Bacteria

Enrichment culture technique leads to the discovery of six presumptive TPH-degrading bacteria. Identification and characterization tests using morphological, biochemical and molecular techniques have successfully isolated Pseudomonas aeruginosa (UMAS1PF), Serratia marcescens (UMAS2SF) and Klebsiella spp. (UMAS3KF). All strains were able to use crude oil as sole carbon and energy source for their growth since they were able to survive in Minimal Salt medium supplemented with 1% (v/v) crude oil. Growth study showed that they produced the highest cell counts on the third or fourth day by 108 – 1011 CFU/ml. Six artificial consortium inoculums have been produced from the growth study. Gas chromatography analysis showed that all isolates had the ability to degrade aliphatic hydrocarbon with 100% degradation of nC19 – C24. Among the isolates, UMAS2SF was the best and fastest n-alkane degrader with degradation percentage between 55 – 90% of n-C14 – C18 in 14 days. This was followed by UMAS1PF and UMAS3KF with 11 – 82% and 1.3% degradation, respectively. Enhancement study showed that plot with inoculum and NPK addition successfully enhanced n-alkane degradation. Plot A2:B3+NPK degraded n-alkane the fastest followed by plot treated by C+NPK, A1:B2, B+NPK and A2:B3. Result showed that UMAS1PF was the best PAHs degrader as most of the high molecular weight PAHs was degraded. In the enhancement study, the plot amended with A2:B3 showed the highest PAHs degradation, followed by plots A1:B2, A3:B1:C2 and A1:C3 that was assigned as the third, fourth and fifth best in mineralizing PAHs, respectively.

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