Bioremediation of Oily Contaminated Soil Through Biostimulation of Indigenous Soil Microbial Community at The Sahara Desert

Author(s):  
Eduardo Carlos Ercoli ◽  
Gonzalo Carrillo ◽  
Omar El Mansuri
2016 ◽  
Vol 51 (4) ◽  
pp. 364-370 ◽  
Author(s):  
Jiřina Száková ◽  
Jitka Havlíčková ◽  
Adéla Šípková ◽  
Jiří Gabriel ◽  
Karel Švec ◽  
...  

Chemosphere ◽  
2021 ◽  
Vol 275 ◽  
pp. 130062
Author(s):  
Luge Rong ◽  
Xuehao Zheng ◽  
Belay Tafa Oba ◽  
Chenbo Shen ◽  
Xiaoxu Wang ◽  
...  

2019 ◽  
Vol 9 (18) ◽  
pp. 3773 ◽  
Author(s):  
Manoharan Joe ◽  
Ram Gomathi ◽  
Abitha Benson ◽  
Devaraj Shalini ◽  
Parthasarathi Rengasamy ◽  
...  

In the present study, a combined treatment strategy involving the addition of rhamnolipid, rhamnolipid-producing bacteria (Shewanella sp. BS4) and a native soil microbial community for the remediation of hydrocarbon-contaminated soil under pilot-scale conditions was adopted. The isolate BS4 (rhl+), demonstrating the highest emulsification activity and surface tension reduction efficiency, was identified based on 16 S rDNA sequencing as Shewanella sp. strain. Growth conditions for rhamnolipid production were optimized based on Central Composite Design (CCD) as 2.9% crude oil, a 54 × 106 CFU g−1 inoculation load of soil, a temperature of 30.5 °C, and a pH of 6.7. In situ bioremediation experiments, conducted using hydrocarbon-contaminated soil treated with the combination of rhamnolipid and rhamnolipid-producing bacteria, showed that the inoculated Shewanella sp. BS4, along with the indigenous soil microbial community, supported the highest hydrocarbon-degrading bacterial population and soil respiration activity, and this treatment resulted in 75.8% hydrocarbon removal efficiency, which was higher compared to contaminated soil devoid of any treatment.


2013 ◽  
Vol 807-809 ◽  
pp. 353-360 ◽  
Author(s):  
Hai Hua Jiao ◽  
Zhi Hui Bai ◽  
Ying Liu ◽  
Kai Wang ◽  
Zhan Bin Huang

A greenhouse pot test, in which wheat, cabbage, spinach were cultivated separately in petroleum contaminated soil with and without super absorbent polymer (SAP), was conducted to evaluated the effect of plants and SAP on soil microbiological properties. phospholipid fatty acids (PLFAs) profiles were analyzed to reveal the microbial communities. As a measure of the functional activity of soil microbial community, the ratio of degraded to total petroleum hydrocarbon (TPH) in soil was estimated. The results indicated that SAP had an important effect on the soil microbial community and its degrading TPH activities. First, the principal component analysis (PCA) of the PLFA signatures revealed marked changes between soil with SAP and without SAP. In addition, the total amount and the profile of PLFA were significantly different between the untreated and SAP-amended soils. Using PLFA patterns as a biomarker, it was found that gram-positive bacteria (G+) were more sensitive to SAP than gram-negative bacteria (G-), and the biomass of G+ was higher in soil with SAP than in that without SAP. Second, the crop could stimulate the growth of soil microorganisms; however, the differences depended clearly on the crop species. The G+ and G- biomass was increased in cabbage, spinach soil containing SAP, but was decreased in wheat soils. The population of fungi was increased in cabbage and spinach soils containing SAP, but was decreased in wheat soil with SAP. The population of actinomycetes was decreased in all soils with SAP. Third, the ratio of degraded to TPH was slightly increased in soil with SAP treatment, but a significant change depended on the crop species. In total, 12 different PLFAs were identified, including saturated, monounsaturated, branched, and polyunsaturated species. There was a clear difference in the PLFAs composition between soils with and without SAP.


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