3D Visualization and Classification of Pore Structure and Pore Filling in Gas Shales

Author(s):  
Carl Sisk ◽  
Elizabeth Diaz ◽  
Joel Walls ◽  
Avrami Grader ◽  
Michael Suhrer
eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Timothy J Duerr ◽  
Ester Comellas ◽  
Eun Kyung Jeon ◽  
Johanna E Farkas ◽  
Marylou Joetzjer ◽  
...  

Measuring nascent macromolecular synthesis in vivo is key to understanding how cells and tissues progress through development and respond to external cues. Here we perform in vivo injection of alkyne- or azide-modified analogs of thymidine, uridine, methionine, and glucosamine to label nascent synthesis of DNA, RNA, protein, and glycosylation. Three-dimensional volumetric imaging of nascent macromolecule synthesis was performed in axolotl salamander tissue using whole-mount click chemistry-based fluorescent staining followed by light sheet fluorescent microscopy. We also developed an image processing pipeline for segmentation and classification of morphological regions of interest and individual cells, and we apply this pipeline to the regenerating humerus. We demonstrate our approach is sensitive to biological perturbations by measuring changes in DNA synthesis after limb denervation. This method provides a powerful means to quantitatively interrogate macromolecule synthesis in heterogenous tissues at the organ, cellular, and molecular levels of organization.


2014 ◽  
Vol 962-965 ◽  
pp. 34-40
Author(s):  
Ning Yang ◽  
Shu Heng Tang ◽  
Song Hang Zhang ◽  
Jun Jie Yi

Gas shales have a complex pore structure. Using mercury porosimetry and nitrogen adsorption experiment on shale of Longtan Formation in southeastern of Hunan, the pore structure characteristics were contrast analyzed, influencing factors and its impact on reservoir-forming were discussed. Longtan Formation shale is composed of nanopores, include the cylinder pores with two ends open and parallel-plate pores with four sides open. The efficiency of mercury ejection ranges 31.45%~63.82%, 51.94% on average, pores uniformity is well. The size of nanopores is 5~30nm, taking up 94.74% of the total volume and 98.08% of specific surface area. Brittle minerals content is high, as an important parameter influencing pore development. The nanopores have a strong ability to absorb gas, methane molecule exist in a structured way.


Author(s):  
Yuanyuan Tian ◽  
◽  
Qing Chen ◽  
Changhui Yan ◽  
Hucheng Deng ◽  
...  

2020 ◽  
Vol 66 (1) ◽  
Author(s):  
Eun-Suk Jang ◽  
Jee-Hoon Yuk ◽  
Chun-Won Kang

Abstract The purpose of this study is to analyze the pore structure for the heartwood, intermediate wood, and sapwood sections in three species of softwood (hinoki, Douglas fir, and hemlock) and to investigate the correlation of gas permeability depending on pore structure. For this study, gas permeability and pore size were measured by capillary flow porometry, and classification of a novel method was performed to determine the type of pores (through pores, blind pores, and closed pores) based on International Union of Pure and Applied Chemistry (IUPAC). Gas permeability, through pore porosity, and pore size increased from heartwood to sapwood. The results of multiple regression analysis showed that through pore porosity, mean pore size, and bulk density were significant factors affecting gas permeability.


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