scholarly journals Effect of traditional food preservatives on biofilm formation by food pathogens

Author(s):  
Khan, M. N. ◽  
Mirani, Z. A. ◽  
Wang, J. ◽  
Lin, H. ◽  
Khan, S. I.
2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Nasser A. Al-Shabib ◽  
Fohad Mabood Husain ◽  
Faheem Ahmed ◽  
Rais Ahmad Khan ◽  
Iqbal Ahmad ◽  
...  

Abstract Bacterial spoilage of food products is regulated by density dependent communication system called quorum sensing (QS). QS control biofilm formation in numerous food pathogens and Biofilms formed on food surfaces act as carriers of bacterial contamination leading to spoilage of food and health hazards. Agents inhibiting or interfering with bacterial QS and biofilm are gaining importance as a novel class of next-generation food preservatives/packaging material. In the present study, Zinc nanostructures were synthesised using Nigella sativa seed extract (NS-ZnNPs). Synthesized nanostructures were characterized hexagonal wurtzite structure of size ~24 nm by UV-visible, XRD, FTIR and TEM. NS-ZnNPs demonstrated broad-spectrum QS inhibition in C. violaceum and P. aeruginosa biosensor strains. Synthesized nanostructures inhibited QS regulated functions of C. violaceum CVO26 (violacein) and elastase, protease, pyocyanin and alginate production in PAO1 significantly. NS-ZnNPs at sub-inhibitory concentrations inhibited the biofilm formation of four-food pathogens viz. C. violaceum 12472, PAO1, L. monocytogenes, E. coli. Moreover, NS-ZnNPs was found effective in inhibiting pre-formed mature biofilms of the four pathogens. Therefore, the broad-spectrum inhibition of QS and biofilm by biogenic Zinc oxide nanoparticles and it is envisaged that these nontoxic bioactive nanostructures can be used as food packaging material and/or as food preservative.


2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
¹Hind H. Muunim ◽  
Muna T Al-Mossawei ◽  
Mais Emad Ahmed

Biofilms formation by pathogens microbial Control considered important in medical research because it is the hazarded virulence factor leading to becoming difficult to treat because of its high resistance to antimicrobials. Glycopeptide antibiotic a (Vancomycin) and the commercial bacteriocin (Nisin A) were used to comparative with purification bacteriocin (MRSAcin) against MRSA biofilm. One hundred food samples were collected from Baghdad markets from July 2016 to September 2016, including (cheese, yogurt, raw milk, fried meat, grilled meat, and beef burger). All samples were cultures; S. aureus was confirmation by macroscopic culture and microscopic examination, in addition to biochemical tests. Methicillin resistance S. asureus (MRSA) were identification by antibiotic sensitivity test (AST), Vitek 2 system. The result shown the 60(60%) isolate were identified as S. aureus and 45(75%) gave positive result as MRSA isolate, M13 isolate was chosen as MRSA isolates highest biofilm formation for treatment with MRSAcin, Nisin A(bacteriocin) and Vancomycin (antibiotic) to compared the more antimicrobial have bacteriocidal effect. The sensitivity test uses to determine the effect of MRSAcin, Nisin A, and Vancomycin MIC on MRSA planktonic cell by (WDA). The new study shows the impacts of new kind Pure Bacteriocins (MRSAcin) from methicillin-resistant S. aureus (MRSA) highly effects then (Vancomycin and Nisin A) at different concentration. In a current study aimed to suggest new Bacteriocin is potent highly for the treatment of resistant bacteria biofilm infections in food preservatives


2008 ◽  
Vol 12 (3) ◽  
pp. 203-208 ◽  
Author(s):  
Nicole Birgit Arweiler ◽  
Ronaldo Lenz ◽  
Anton Sculean ◽  
Ali Al-Ahmad ◽  
Elmar Hellwig ◽  
...  

LWT ◽  
2018 ◽  
Vol 97 ◽  
pp. 1-8 ◽  
Author(s):  
Alexandre Lamas ◽  
Alba María Paz-Mendez ◽  
Patricia Regal ◽  
Beatriz Vazquez ◽  
José Manuel Miranda ◽  
...  

2022 ◽  
Vol 12 (2) ◽  
pp. 660
Author(s):  
Despina Vougiouklaki ◽  
Theofania Tsironi ◽  
Joseph Papaparaskevas ◽  
Panagiotis Halvatsiotis ◽  
Dimitra Houhoula

Lactic acid bacteria (LAB) play an important role as natural food preservatives. However, the characterization of the variety of their metabolites is limited. The objective of this study was to determine the production of specific metabolites of Lacticaseibacillus rhamnosus, Levilactobacillus brevis and Lactiplantibacillus plantarum by an optimized liquid chromatography with an ultraviolet/diode detection (HPLC-UV/DAD) method and to investigate their potential antimicrobial activity against specific food pathogens. Based on the results of this study, the main metabolites detected in Levilactobacillus brevis were 103.4 μg mL−1 DL-p-Hydroxyphenyllactic acid (OH-PLA) and 2.59 μg mL−1 vanillic acid, while 216.2 μg mL−1 OH-PLA, 19.0 μg mL−1 salicylic acid, 3.7 μg mL−1 vanillic acid, 6.9 μg mL−1 ferulic acid, 4.2 μg mL−1 benzoic acid and 1.4 μg mL−1 4-Hydrocinnamic acid were identified in the Lactiplantibacillus plantarum strain and 147.6 μg mL−1 OH-PLA and 4.9 μg mL−1 ferulic acid were identified in Lacticaseibacillus rhamnosus. This study provides alternative approaches for the molecules involved in the antimicrobial activity of food microorganism fermentation. These molecules may be used as antimicrobial ingredients in the food industry instead of conventional chemical preservatives.


RSC Advances ◽  
2015 ◽  
Vol 5 (94) ◽  
pp. 77168-77174 ◽  
Author(s):  
Shankar Subramaniam ◽  
Narendran Rajendran ◽  
Sai brinda Muralidharan ◽  
Gayathri Subramaniam ◽  
Ravikumar Raju ◽  
...  

Dual role of commercially important nutraceuticals from plants that potentiate the therapeutic effect of commercial antibiotics to combat food pathogens.


2013 ◽  
Vol 18 (3) ◽  
pp. 829-838 ◽  
Author(s):  
Nicole Birgit Arweiler ◽  
Lutz Netuschil ◽  
Daniel Beier ◽  
Sebastian Grunert ◽  
Christian Heumann ◽  
...  

2003 ◽  
Vol 16 (6) ◽  
pp. 223-229 ◽  
Author(s):  
Jari Vartiainen ◽  
Eija Skytta ◽  
Jouni Enqvist ◽  
Raija Ahvenainen

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zeinab Rezaei ◽  
Saeid Khanzadi ◽  
Amir Salari

AbstractCurrently, the health benefits of probiotic bacteria are well known, and this has taken up a great deal of space in food science and health, both research and operational. On the other hand, anti-biofilm properties on food pathogens in the food and pharmaceutical industries have created an attractive challenge. This study aimed to describe the inhibitory activity of cell-free supernatants (CFS), planktonic cells, and biofilm form of lactobacilus strains (L. rhamnosus and L. plantarum) against food pathogens such as Pseudomonas aeruginosa and Listeria monocytogenes. Anti-bacterial activities of the CFS of lactobacillus strains were assessed by the microplate method and via violet staining. Evaluation of the antagonistic activity of planktonic cells and biofilm of LAB were performed by the spread plate method. The results showed the incubation time of 48 h was the best time to produce biofilm. Although the planktonic states reduce the pathogens bacterial about 1 –1.5 log, but in biofilm forms, decreased L. monocytogenes about 4.5 log compared to the control, and in the case of P. aeruginosa, a growth reduction of about 2.13 log was observed. Furthermore, biofilm formation of L. monocytogenes in the presence of L. rhamnosus cell-free supernatant was more weakly than L. plantarum CFS, but their CFS effect on reducing the bacterial population of P. aeruginosa was the same. According to the study, biofilm produced by probiotic strains can be considered a new approach for biological control. Also, cell-free supernatant can be used as postbiotic in the food and pharmaceutical industries.


2008 ◽  
Vol 53 (8) ◽  
pp. 765-772 ◽  
Author(s):  
Ali Al-Ahmad ◽  
Margit Wiedmann-Al-Ahmad ◽  
Thorsten Mathias Auschill ◽  
Marie Follo ◽  
Gabriele Braun ◽  
...  

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