scholarly journals Characterization and Experimental Infection of Vibrio harveyi Isolated from Diseased Asian Seabass (Lates calcarifer)

2012 ◽  
Author(s):  
Ransangan, J. ◽  
Lal, T. M. ◽  
Al-Harbi, A. H.
Keyword(s):  
Experimental Infection ◽  
Vibrio Harveyi ◽  
Lates Calcarifer ◽  
Asian Seabass

scholarly journals Effects of skin abrasion in immersion challenge with Vibrio harveyi in Asian seabass Lates calcarifer fingerlings

2020 ◽  
Vol 137 (3) ◽  
pp. 167-173 ◽  
Author(s):  
YK Chin ◽  
MY Ina-Salwany ◽  
M Zamri-Saad ◽  
MNA Amal ◽  
A Mohamad ◽  
...  
Keyword(s):  
Vibrio Harveyi ◽  
Lates Calcarifer ◽  
Asian Seabass ◽  
Skin Abrasion

scholarly journals Laboratory and Field Assessments of Oral Vibrio Vaccine Indicate the Potential for Protection against Vibriosis in Cultured Marine Fishes

Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 133
Author(s):  
Aslah Mohamad ◽  
Fathin-Amirah Mursidi ◽  
Mohd Zamri-Saad ◽  
Mohammad Noor Azmai Amal ◽  
Salleh Annas ◽  
...  
Keyword(s):  
Vaccine Candidate ◽  
Vibrio Harveyi ◽  
Primary Vaccination ◽  
Experimental Period ◽  
Lates Calcarifer ◽  
Asian Seabass ◽  
Vaccination Trial ◽  
Hybrid Grouper ◽  
Antibody Titres ◽  
Group 2

Vibriosis is one of the most common threats to farmed grouper; thus, substantial efforts are underway to control the disease. This study presents an oral vaccination against multiple Vibrio spp. in a marine fish with double booster immunisation. The Vibrio harveyi strain VH1 vaccine candidate was selected from infected groupers Epinephelus sp. in a local farm and was formalin inactivated and combined with commercial feed at a 10% ratio (v/w). A laboratory vaccination trial was conducted for seventy days. The induction of IgM antibody responses in the serum of Asian seabass Lates calcarifer immunised with the oral Vibrio harveyi strain VH1 was significantly (p < 0.05) increased as early as week one post-primary vaccination. Subsequent administration of the first and second booster for 5 consecutive days, starting on days 14 and 42, respectively, improved the specific antibody level and reached a highly significant (p < 0.05) value at days 35 and 49 before slightly decreasing from day 56 onwards. Antibody titres of the control unvaccinated group remained relatively stable and low throughout the experimental period. At the end of the 70-day vaccination trial, 23 days post final boost, an intraperitoneal challenge with a field strain of Vibrio harveyi, V. alginolyticus, and V. parahaemolyticus was carried out. Our challenge study showed that oral Vibrio harveyi strain VH1 vaccine candidate could induce significant protection, with an RPS of 70–80% against different Vibrio species. Thereafter, a field trial was conducted in a mariculture farm to study the effect of field vaccination using the oral Vibrio harveyi strain VH1 vaccine candidate. A total of 3000 hybrid grouper juveniles were divided into two groups in triplicate. Fish of Group 1 were not vaccinated, while Group 2 were vaccinated with the feed-based vaccine. Vaccinations were carried out on days 0, 14, and 42 via feeding the fish with the vaccine at 4% body weight for 5 consecutive days. At the end of the study period, the fish survival rate was 80% for the vaccinated group, significantly (p < 0.05) higher than the 65% seen in the control unvaccinated group. Furthermore, the vaccinated fish showed significantly (p < 0.05) better growth performances. Therefore, the oral Vibrio vaccine from the inactivated Vibrio harveyi strain VH1 is a potential versatile vaccine candidate that could stimulate good immune responses and confer high protection in both Asian seabass, Lates calcarifer, and farm hybrid grouper Epinephelus fuscoguttatus × Epinephelus lanceolatus.

scholarly journals Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer

2019 ◽  
Vol 51 (1) ◽  
pp. 389-399 ◽  
Author(s):  
Yong Kit Chin ◽  
Nurhidayu Al‐saari ◽  
Zarirah Zulperi ◽  
Aslizah Mohd‐Aris ◽  
Annas Salleh ◽  
...  
Keyword(s):  
Vibrio Harveyi ◽  
Lates Calcarifer ◽  
Asian Seabass

Characterization of red-spotted grouper nervous necrosis virus isolated from ovarian fluids of asymptomatic wild Asian seabass, Lates calcarifer

Aquaculture ◽  
2021 ◽  
pp. 736846
Author(s):  
Venkata Satyanarayana Nallala ◽  
M. Makesh ◽  
K. Radhika ◽  
T. Sathish Kumar ◽  
P. Raja ◽  
...  
Keyword(s):  
Nervous Necrosis Virus ◽  
Necrosis Virus ◽  
Lates Calcarifer ◽  
Asian Seabass
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