scholarly journals Determination of human epidermal growth factors in cultured media of Escherichia coli by high performance liquid chromatography.

1987 ◽  
Vol 3 (5) ◽  
pp. 445-448 ◽  
Author(s):  
Tomonori HAYASHI ◽  
Shunji SAKAMOTO ◽  
Toru FUWA ◽  
Ikue MORITA ◽  
Hisanobu YOSHIDA
Keyword(s):  
Escherichia Coli ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Growth Factors ◽  
High Performance ◽  
Epidermal Growth Factors ◽  
Epidermal Growth

(6R)-5,6,7,8-Tetrahydro-L-Monapterin from Escherichia coli, a Novel Natural Unconjugated Tetrahydropterin

2002 ◽  
Vol 383 (2) ◽  
pp. 325-330 ◽  
Author(s):  
K. Ikemoto ◽  
T. Sugimoto ◽  
S. Murata ◽  
M. Tazawa ◽  
T. Nomura ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Side Chain ◽  
Air Oxidation ◽  
Stereochemical Structure ◽  
Natural Sources ◽  
Aromatic Derivative

Abstract The structure of the major tetrahydropterin in Escherichia coli was determined as (6R)-5,6,7,8-tetrahydroLmonapterin, i. e. (6R)-2-amino-5,6,7,8-tetrahydro 6-[(1S,2S)-1,2,3-trihydroxypropyl]pteridin-4(3H)one. Although the stereochemical structure of the trihydroxypropyl side chain has been determined previously by fluorescence detected circular dichroism analysis on its aromatic derivative, the most important configuration at C(6) has not been clarified. The major difficulties for the determination of the chirality were instability toward air oxidation and very low concentration of the tetrahydropterin derivative. In the present study, the C(6)configuration was determined as R by comparing its stable hexaacetyl derivative with authentic (6R) and (6S)hexaacetyl-5,6,7,8- tetrahydroLmonapterins by high performance liquid chromatography (HPLC) and HPLCmass spectrometry (LCMS). (6R)-5,6,7,8-TetrahydroLmonapterin is a new unconjugated tetrahydropterin from natural sources.

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