scholarly journals 31. Histological of Tractus Digestivus of Domestical Catfish (Clarias batracus)

2019 ◽  
Vol 13 (2) ◽  
Author(s):  
Aliman Irfandi ◽  
Cut Dahlia Iskandar ◽  
Zainuddin Zainuddin ◽  
Dian Masyitha ◽  
Fitriani Fitriani ◽  
...  

Domestical catfish is grouped into omnivora, although the fish is tended into carnivora.  The aim of the present study was to investigate histology of alimentary tract of domestical catfish (Clarias batrachus). In order to determine alimentary tract of domestical catfish, oesophagus, gastric and intestines is investigated by Haematoxilin Eosin. Histology exploration was performed each layer of alimentary tract’s wall consist of lamina epithelia, propria, and muscularis mucosa. Collagen connective tissue was developed tunica submucosa. The muscle of tunica muscularis was performed circular and elongated. Dense connective tissue and blood vessels was located in the tunica serosa. Histology exploration revealed has stratified squamous epithelium, mucosit cells, eosinophilic club cells on the lamina epithelia, and taste buds on the lamina propria in oesophagus. Gastric was establised simple prismatic cells, and glands of gastric on the lamina propria. Furthermore intestine was showed villi and goblet cells. There were not showed Brunner and Liberkhun gland. In conclusion, histology of alimentary tract was revealed from tunica mucosa, submucosa, and serosa.

2015 ◽  
Vol 87 (2) ◽  
pp. 867-878 ◽  
Author(s):  
Marcella L. Dos Santos ◽  
Fábio P. Arantes ◽  
Kleber B. Santiago ◽  
José E. Dos Santos

The digestive tracts of 44 specimens of Schizodon knerii were studied using anatomical, histological and histochemical techniques. The mouth has terminal position, the lip epithelium is squamous stratified with mucous, claviform cells and taste buds, teeth have an incisive form and the tongue has a stratified squamous epithelium with mucous cells and taste buds. The oropharynx cavity is formed by gill apparatus and pharyngeal teeth. The oesophagus presented pleated mucosa, a stratified squamous epithelium with mucous cells, oesophageal glands and taste buds. The stomach presented cardiac, fundic and pyloric regions, simple prismatic epithelium with tubular glands, with none in the pyloric region. The intestine contains 11-15 pyloric caeca, a simple prismatic epithelium with brush border, goblet cells and lymphocytes. Mucosal cells, oesophageal glands and goblet cells reacted positively to PAS, amylase + PAS, Ab pH 2.5 and Ab pH 0.5. Gastric prismatic cells reacted positively to PAS, amylase + PAS, but only those in the pyloric region reacted positively to Ab pH 2.5 and Ab pH 0.5. The results improve the understanding of the anatomy of S. knerii feeding habits and the presence of mucosubstances in the epithelium, highlights the importance of glycoproteins for passing food through the digestive tract.


Author(s):  
J. S. Alexandrawicz

A specimen of the red mullet (Mullus surmuletus L.), caught in Plymouth waters in October 1949, was found to have tumours projecting externally on the pectoral fins. They were spherical or elliptical in shape, the largest being 20 mm. long. On the suggestion of Mr G. A. Steven some of these outgrowths were cut out and given to the writer for microscopic examination.When examined fresh they appear to consist of small, spherical, cyst-like bodies of various diameters, but there was no obvious clue as to their nature. After fixing in Bouin and sectioning, they are seen to be composed of cells of various sizes (PI. I, figs. 1–4). It can be seen that these cells lie close to one another, leaving only a little space for the embedding connective tissue which is characterized by the abundance of its own small cells. The blood vessels met with in this tissue contain fish erythrocytes. The whole is covered by stratified squamous epithelium (PI. I, fig. 2).


2010 ◽  
Vol 36 (1) ◽  
pp. 3-10
Author(s):  
André Alan Nahas ◽  
Paula Dechichi ◽  
Denildo de Magalhães ◽  
Andreia Espíndola Vieira

Abstract Osseointegrated dental implants are inserted into the alveolar ridge, and for them to function as tooth replacements, the surrounding tissues need to adapt to them. Just as with teeth, dental implants traverse the oral mucosa and have access to the contaminated environment of the oral cavity. Therefore, periodontal and peri-implant tissues are important for establishing a protective barrier. The aim of the present study was to perform a histologic analysis of the mucosa surrounding osseointegrated implant cover screws. For this study, 17 mucosal specimens were obtained from 12 patients during the second surgical session for implant exposure to the oral environment. After histologic preparation, specimens were sectioned perpendicularly to the mucosal surface to a thickness of about 3 µm, stained with 1% toluidine blue, and examined under light microscopy. All specimens showed a keratinized, stratified, squamous epithelium with well-defined strata. In the lamina propria, unorganized dense connective tissue was noted in the reticular layer, and in 4 samples, a chronic inflammatory infiltrate was seen in this region. The papillary layer presented tall connective papillae consisting of loose connective tissue. The results of this study confirm the hypothesis that the mucosa that conceals osseointegrated implant cover screws has the same morphologic characteristics as the alveolar masticatory mucosa. Furthermore, clinical conditions of normality in peri-implant tissues may not coincide with situations of histologic normality.


2015 ◽  
Vol 2015 ◽  
pp. 1-4
Author(s):  
Nurcan Tezci ◽  
Suleyman Emre Meseli ◽  
Burcu Karaduman ◽  
Serap Dogan ◽  
Sabri Hasan Meric

Background. Oral fibromas are benign, asymptomatic, smooth surfaced, firm structured tumoral lesions that originate from gingival connective tissue or periodontal ligament. Histologically, they are nodular masses characterized by a dense connective tissue, surrounded by stratified squamous epithelium.Case Report. This case report includes the clinical, radiographical, and histological findings and periodontal treatment of a 38-year-old female patient having painless swelling on the gingiva. Intraoral examination revealed a fibrotic, sessile, smooth surfaced gingival overgrowth interdentally between the teeth #13 and #14. Radiographical findings were normal. Initial periodontal treatment (IPT) was applied including oral hygiene instructions, scaling, and root planing. Following IPT, the lesion (0.7 × 0.6 × 0.4 cm) was excised and examined histopathologically. Subsequently, flap operation was performed to have an access to alveolar bone. Surgical site was reconstructed with free gingival graft obtained from hard palate. Hematoxylin-eosin stained sections revealed a nodular mass composed by dense collagen fibers in lamina propria covered by a stratified squamous epithelium, which were consistent with fibroma. Gingival healing was uneventful and without any recurrence during the 12-month follow-up.Conclusions. In order to achieve optimal functional and aesthetical outcomes, free gingival graft can be used for the reconstruction of the wound site after the excision of the fibroma.


Development ◽  
1961 ◽  
Vol 9 (3) ◽  
pp. 370-384
Author(s):  
C. B. McLoughlin

It is well established that in the developing chick the underlying mesenchyme initiates the appearance of specific epidermal derivatives, e.g. feathers (Sengel, 1956), claws (Cairns & Saunders, 1954), and the preen gland (Gomot, 1958). On the other hand, it is not yet known to what extent the epidermis is independent of mesenchymal intervention for its basic differentiation into a stratified, squamous epithelium. Sobel's (1958) work on the 8-day chick pituitary suggests that the differentiation and multiplication of certain epithelial cells cannot proceed in the absence of mesenchymal elements. She found that the isolated epithelial cells of the hypophysial rudiment survived but were unable to differentiate or multiply; when associated with perichondrial fibroblasts, however, they resumed mitosis and produced typical α and β cells. In the first part of the present investigation, experiments were made to see whether the embryonic epidermis, like the hypophysial epithelium, requires the resence of fibroblasts to enable it to grow and differentiate, or whether it can proliferate, acquire its characteristic squamous structure and keratinize, when isolated and cultivated in the absence of connective tissue.


2020 ◽  
Author(s):  
Varsha Gupta ◽  
Muneer Mohhamad Farooqui ◽  
Ajay Prakash Sharma ◽  
Archana Pathak

AbstractThe study was conducted on 36 developing reticulum from healthy and normal embryos/ foeti of Indian goat. Embryos/foeti were assigned into three groups. Histo-differentiation of reticulum of goat stomach took place at 38 days of gestation. The wall of foetal reticulum was made up of three strata i. e. epithelium, pleuripotent blastemic tissue and serosa up to 49 days of fetal age and definite four layers viz. epithelium, propria-submucosa, tunica muscularis and serosa were observed first at 51 days of gestation. Upto 100 days of gestation the reticulum was lined by undifferentiated stratified epithelium and thereafter gradually transformed into stratified squamous epithelium. Keratinization was noticed at term. Primary and secondary reticular crests made their appearance at 60 and 112 days of gestation, respectively. Corial papillae were detected in primary and secondary reticular crests at 121 and 145 days of gestation, respectively. Near term, the core of the reticular crest also contained muscularis mucosae in its proximal 1/3rd region while core of the corial papillae contained only lamina propria. Reticular, collagen and elastic fibers came into sight at 46, 100 and 134 days of gestation, respectively.Summary statementThe study was conducted on intrauterine reticulum of Indian goat. From this study it can be concluded that the histogenesis of reticulum was almost completed in prenatal life. However, to become functional it still required more time as the relative sizes of reticulum and process of keratinization were yet to be completed.


2014 ◽  
Vol 6 (3) ◽  
pp. 134-136
Author(s):  
Nilamadhab Prusty

ABSTRACT Epidermoid cyst occurring within the tongue is rare. A 5-year- old male child was brought to outpatient department (OPD) with a tongue mass which was gradually increasing in size. There was associated difficulty in speech and mastication as the swelling increased in size. Intraoral examination revealed moderately tender, fluctuant and enlarged tongue. A diagnosis of dermoid cyst was made and the patient was booked for surgery. Excision of the cyst was done under general anesthesia. Postoperative histopathology was done. The histopathological findings confirm the diagnosis of an epidermoid cyst, characterized by the presence of: (i) a cyst cavity lined by stratified squamous epithelium with keratinization on the surface; and (ii) connective tissue with a mild inflammation. The proposed treatment was considered successful as the case was solved and there was no recurrence. How to cite this article Naik C, Prusty N. Epidermoid Cyst of Tongue: A Case Presentation. Int J Otorhinolaryngol Clin 2014;6(3):134-136.


Development ◽  
1988 ◽  
Vol 104 (3) ◽  
pp. 431-446 ◽  
Author(s):  
P.V. Senior ◽  
D.R. Critchley ◽  
F. Beck ◽  
R.A. Walker ◽  
J.M. Varley

In situ hybridization (ISH) and immunocytochemistry were used to localize sites of synthesis and deposition of the basement membrane glycoprotein laminin during development in the postimplantation mouse embryo and extraembryonic membranes. In addition, similar studies were performed on postnatal viscera during the first 20 days after birth. Up to 10 days post coitum, embryonic laminin synthesis was confined to parietal endoderm. In maternal tissue, intense laminin mRNA expression was detected in decidual cells in the mesometrial and antimesometrial endometrium at 5–7 days. At 10 days, uniform expression was still seen within the mesometrial endometrium, with higher levels around migrating trophoblast, but in the antimesometrial aspect expression was restricted to the basal zone. High levels of mRNA expression persisted in parietal endoderm throughout gestation but much lower levels were detected in visceral yolk sac. In the mature placenta, laminin mRNA expression was also found associated with fetal vessels in the labyrinth and giant cells at the fetal/maternal boundary. In the embryo, the external limiting membrane of the cerebral vesicles and spinal cord stained for laminin protein and detectable mRNA was found in the pia mater. Growing peripheral nerves and dorsal and ventral root fibres expressed laminin mRNA and stained for laminin protein. Laminin mRNA expression was found in ureteric buds and nephrogenic vesicles (but not in metanephric blastema) during early prenatal kidney development, and in glomeruli, Bowman's capsule, loops of Henle and collecting duct cells at later stages of development, and after birth. All these structures possessed laminin-rich basement membrane (BM). Laminin mRNA expression fell to below detectable levels in the kidney around weaning. In the gut, laminin expression and protein staining was confined to the muscularis externa and the lamina propria during embryogenesis. After birth, the muscularis externa, muscularis mucosa and lamina propria cells corresponding to fibroblasts had detectable laminin mRNA, but in adult gut no laminin mRNA could be demonstrated in any cell type. In liver, low levels of laminin mRNA were seen in the capsule and in periportal connective tissue. After birth, laminin mRNA was associated with intrahepatic bile channels; no laminin mRNA was detected in the parenchyma and protein deposition was restricted to blood sinus BM. In the adult liver, no laminin mRNA was detected in any cell type. The developing heart showed uniform expression of laminin mRNA from 12 days to before birth. Postnatally, labelling was restricted to connective tissue cells.


1971 ◽  
Vol 49 (2) ◽  
pp. 241-248 ◽  
Author(s):  
F. Ramprashad ◽  
S. Corey ◽  
K. Ronald

The gross and microscopic structure of the auditory meatus of the harp seal (Pagophilus groenlandicus) is described from dissections and serial sections. The meatus bends ventrally from the oval external orifice, posteroventrally, ventrally, and finally medially into the cranium. The ventrally directed wall of the meatus is membranous, the posteroventral and ventrally directed wall is incompletely wrapped by cartilage, and the medially directed wall is bony. The areas between sections of the cartilaginous wall ate completed by connective tissue. The meatus is closed internally by the intrinsic helicis and antitragicus auricular muscles. The external orifice is closed by the superficial auricular muscles. The auditory meatus is lined with stratified squamous epithelium, sebaceous glands, and ceruminous glands. Longitudinal blood sinuses and elastic fibers in the hypodermis may function as a pressure regulating device during diving. Blood sinuses are most prominent in the bony region of the meatus and extend into the tympanic sulcus of the pars tensa. Blood sinuses engorged with blood and a closed meatus may enhance transmission of sound to the tympanic membrane when the seal is under water.


2010 ◽  
Vol 10 (4) ◽  
pp. 307-313 ◽  
Author(s):  
Boban Džunić ◽  
Predrag Jovanović ◽  
Dragan Veselinović ◽  
Aleksandar Petrović ◽  
Ivan Stefanović ◽  
...  

Pterygium internum (external eye layer) shows great recurrence tendency after surgical removal. Its etiology is still unclear and represents a significant problem. The main goal of our study was to explore the interrelationships of pathohistological characteristics of pterygium, namely presence of inflammation, vascularisation degree and fibrinoid changes and on the basis of their analysis to test the possibility of predicting its evolution and recurrence. The analysis was performed on the material taken from 55 patients surgically treated by the technique of Arlt. The specimens were stained using the classical histochemical methods: hematoxylin-eosin (HE), Masson’s trichrome, Gomori’s reticulin stain and PAS technique. Pterygium is mostly covered by conjunctival epithelium, while in the cap region shows morphology of modified stratified squamous epithelium of the cornea. Structural basis of the epithelium is composed of continuous basal lamina and continuous connective fibers underneath. This connective basis shows fibrinoid changes in the form of oval islets of different size, parallel to convexity of pterygium, or is in the form of unified focus. The number, caliber and the type of blood vessels showed excessive variability.Pathohistological analysis of morphological characteristics of pterygium is adequate basis for prediction of recurrences; as they present the biggest concern in treatment of this widely spread disease.


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