scholarly journals 26. GAMBARAN HISTOPATOLOGIS GINJAL TIKUS PUTIH (Rattus novergicus) JANTAN YANG DIINFEKSIKAN Trypanosoma evansi DAN DIBERI EKSTRAK DAUN SERNAI (Wedelia biflora) (Histopathology of Male Rat (Rattus novergicus) Kidney Infected with Trypanosoma evansi and Treated with Sernai Leaves Extract (Wedelia biflora))

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
Yudha Fahrimal ◽  
Rahmiwati R ◽  
Dwinna Aliza
Keyword(s):  
Untreated Control ◽  
Histopathological Examination ◽  
Rat Kidney ◽  
Trypanosoma Evansi ◽  
Male Rats ◽  
Male Rat ◽  
Control Group ◽  
Untreated Control Group ◽  
Stomach Tube ◽  
Treatment Groups

This research was aimed to study histopathology of rat kidney infected with Trypanosoma evansi after administration of sernai leaves extract (Wedelia bifolora). The samples used were kidneys of 25 male rats strain Wistar which were equally divided into 5 treatment groups. Group P1 was uninfected and untreated control, group P2 was infected with 1x103 T. evansi but not sernai leaves extract, rats from groups P3, P4, and P5 were infected with 1x103 T. evansi and given sernai leaves extract with the dose of 30 mg/kg bw, 45 mg/kg bw, and 60 mg/kg bw respectively. The sernai extract was administered orally using a stomach tube for 3 consecutive days. The next day after last treatment, all rats were sacrificed and necropsied. Kidneys were collected for histopathological examination such as degeneration and necrosis of tubules cells, convoluted contortus duct lumen, and adhesion and atrophy of glomerulus. Sernai leaves extract with the dose of 45 mg/kg bw was the most effective dose to prevent kidney damage due to T. evansi infection.

scholarly journals The Effect of Wilow Bark Ekstract Administration on Microscopic Feature of Male Rats (Rattus novergicus) Kidney Infected with Trypanosoma evansi

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Nurdiniyah Nurdiniyah ◽  
Nazaruddin Nazaruddin ◽  
Sugito Sugito ◽  
M. Nur Salim ◽  
Yudha Fahrimal ◽  
...  
Keyword(s):  
Tissue Damage ◽  
Histopathological Examination ◽  
Kidney Tissue ◽  
Trypanosoma Evansi ◽  
Male Rats ◽  
Bark Extract ◽  
Control Group ◽  
Willow Bark ◽  
Microscopic Feature ◽  
Group 2

The aims of this study was to examine the ability of willow bark extract to prevent the kidney tissue damage from T. evansi infection. This study used 25 rats which were randomly divided into 5 treatment groups, each groups consisted of 5 rats. Rats in control group (K0) were not infected with T. Evansi and without administration of willow bark extract. Group 1 (P1) was infected with 103/0,3 ml of T. evansi without willow bark extract administration. Group 2, 3, and 4 were infected with 103/0.3 ml T. evansi and received willow bark extract at dose of 30, 45, and 60 mg/kg bw, respectively. Willow bark extract were administered for 3 days consecutively. On day 4, the rats were sacrificed using chloroform and the kidneys were collected for histopathological examination. Data were analyzed descriptively. The results showed that administration of willow bark extract at doses of 30 and 45 mg/kg bw were able to prevent damage of tubule cells and glomerular renal, while administration of 60 mg/kg bw caused kidney damage, indicated with necrosis in tubular glomerular cells. It could be concluded that the willow bark extract at doses of 30 and 45 mg/kg Bw were effective to prevent tissue damage in kidneys.  Key words: kidneys, willow, Trypanosoma evansi

scholarly journals PENGARUH PEMBERIAN EKSTRAK KULIT BATANG JALOH TERHADAP GAMBARAN MIKROSKOPIS GINJAL TIKUS PUTIH (Rattus novergicus) YANG DIINFEKSI (Trypanosoma evansi The Effect of Wilow Bark Ekstract Administration on Microscopic Feature of Male Rats (Rattus novergicus) Kidney Infected with Trypanosoma evansi)

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Nurdiniyah Nurdiniyah ◽  
Nazaruddin Nazaruddin ◽  
Sugito Sugito ◽  
M Nur Salim ◽  
Yudha Fahrimal ◽  
...  
Keyword(s):  
Tissue Damage ◽  
Histopathological Examination ◽  
Kidney Tissue ◽  
Trypanosoma Evansi ◽  
Male Rats ◽  
Bark Extract ◽  
Control Group ◽  
Willow Bark ◽  
Microscopic Feature ◽  
Group 2

The aims of this study was to examine the ability of willow bark extract to prevent kidney tissue damage from T. evansi infection. This study used 25 rats which were randomly divided into 5 treatment groups, each groups consisted of 5 rats. Rats in control group (K0) were not infected with T. evansi and without administration of willow bark extract. Group 1 (P1) was infected with 103/0,3 ml of T. evansi without willow bark extract administration. Group 2, 3, and 4 were infected with 103/0.3 ml T. evansi and received willow bark extract at dose of 30, 45, and 60 mg/kg bw, respectively. Willow bark extract were administered for 3 days consecutively. On day 4, the rats were sacrificed using chloroform and the kidneys were collected for histopathological examination. Data were analyzed descriptively. The results showed that the administration of willow bark extract at doses of 30 and 45 mg/kg bw were able to prevent damage of tubule cells and glomerular renal, while the administration of 60 mg/kg bw resulted in kidney damage, indicated by necrosis in tubular glomerular cells. It could be concluded that willow bark extract at doses of 30 and 45 mg/kg bw were effective to prevent tissue damage in kidneys.Key words: kidneys, willow, Trypanosoma evansi

scholarly journals GAMBARAN HISTOPATOLOGIS LIMPA TIKUS PUTIH (Rattus novergicus) JANTAN DIINFEKSI Trypanosoma evansi DAN DIBERI EKSTRAK KULIT BATANG JALOH (Salix tetrasperma Robx) (Histopathological finding of Rat (Rattus novergicus) Spleen Infected with Trypanosoma evansi and Treated with Willow Bark (Salix tetrasperma Roxb) Extract)

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Elsa Mariane Ramadani ◽  
Hamdani Budiman ◽  
Sugito Sugito ◽  
Ummu Balqis ◽  
Muhammad Hambal ◽  
...  
Keyword(s):  
Trypanosoma Evansi ◽  
Male Rats ◽  
Histopathological Finding ◽  
Bark Extract ◽  
Control Group ◽  
White Pulp ◽  
Treatment Groups ◽  
Group I ◽  
Normal Spleen ◽  
Willow Bark

The aim of this research was to determine the histopathological finding of rat (Rattus novergicus) spleen infected with Trypanosoma evansi and treated with willow bark (Salix tetrasperma Roxb) extract.This research used 25 male rats divided into four treatment groups and fed ad libitum. Group 0 (K0) was control group, group I (K1) was infected with 103/0.3 ml. T. evansi, group II (K2), III (K3), and IV (K4) were infected with103/0.3 ml T. evansi and treated with willow bark extract 30, 45, and 60 mg/kg bw, respectively. All groups were treated for 3 consecutive days orally using a stomach tube. On day four, rats were euthanized using chloroform and necropsied, then spleen were collected for histophatological examination. The results showed that all spleen on group K1, K2, K3, and K4 showed hyperplasia of white pulp, hemosiderin, and necrosis with different severity levels compared to normal spleen structure. In conclusion, the administration of willow bark extract at the dose of 30 mg/kg bw has a potency to prevent spleen from T. evansi infection. However, the higher the dose of willow bark extract the higher the level of spleen damage.Key words: spleen, T. evansi, willow bark extract

scholarly journals Activities of Several Antimicrobials againstMycobacterium ulcerans Infection in Mice

2000 ◽  
Vol 44 (9) ◽  
pp. 2367-2372 ◽  
Author(s):  
Herve Dega ◽  
Jerome Robert ◽  
Pascale Bonnafous ◽  
Vincent Jarlier ◽  
Jacques Grosset
Keyword(s):  
Body Weight ◽  
Untreated Control ◽  
Generation Time ◽  
Human Infection ◽  
Mycobacterium Ulcerans ◽  
Control Group ◽  
Untreated Control Group ◽  
Treatment Groups ◽  
Lowenstein Jensen ◽  
Observation Period

ABSTRACT Mycobacterium ulcerans inoculated into the footpads of mice at 6 × 103 CFU was shown to have a generation time of 6.5 days when estimated from weekly changes in microscopic counts of acid-fast bacilli (AFB) and 7.5 days when calculated from actual CFU enumerated on Lowenstein-Jensen egg medium incubated at 32°C. Footpads became swollen at week 10 (W10) after infection, and all infected control mice were dead at W15 after infection. Daily (5 days/week) treatment with 100 mg of clarithromycin (CLR)/kg of body weight beginning the day after infection prevented swelling of footpads at W10. When initiation of treatment was delayed until obvious footpad swelling was observed, there was a reduction in both the increase in AFB counts and deterioration of swollen footpads and also a prolonged survival of the mice to W18. Mice infected in the hind footpads with 5 × 105 CFU of M. ulcerans were divided into an untreated control group and six treatment groups that received one of the following therapies for 8 weeks: 100 mg of CLR/kg, 25 mg of minocycline (MIN)/kg, 50 mg of sparfloxacin (SPX)/kg, 10 mg of rifampin (RIF)/kg, 10 mg of rifabutin (RBT)/kg, or 100 mg of amikacin (AMK)/kg. After completion of therapy, treated animals were observed for an additional 17 weeks. All control mice and mice treated with CLR, MIN, or SPX exhibited swollen footpads during the observation period. In contrast, of those animals treated with RIF, RBT, or AMK, none had footpad swelling and all inoculated cultures done after the W17 observation remained negative. These results suggest that RIF, RBT, and AMK may be effective in the treatment of human infection with M. ulcerans.

scholarly journals GAMBARAN HISTOPATOLOGIS HATI TIKUS (Rattus novergicus) YANG DIINFEKSI Trypanosoma evansi SETELAH PEMBERIAN EKSTRAK KULIT BATANG JALOH (Salix tetrasperma Roxb) (Histopathological Changes of Rat (Rattus novergicus) Liver Infected with Trypanosoma evansi and Treated with Willow Tree Bark Extract (Salix tetrasperma Roxb))

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Mulya Fahmi ◽  
Yudha Fahrimal ◽  
Dwinna Aliza ◽  
Siti Aisyah ◽  
Hamdani Budiman ◽  
...  
Keyword(s):  
Histopathological Examination ◽  
Negative Control ◽  
Trypanosoma Evansi ◽  
Tree Bark ◽  
Male Rat ◽  
Bark Extract ◽  
Control Group ◽  
Histopathological Changes ◽  
Hydropic Degeneration ◽  
Willow Tree

This research aimed to determine the effect of willow (Salix tetrasperma Roxb) tree bark extract on histopathological changes of liver of rat (Rattus novergicus) infected with Trypanosoma evansi. Liver of 25 male rat, Wistar strain, were used in this study and all rat were fed with commercial feed ad libitum. This research used completely randomized design (CRD) with 5 treatments, each treatment consisted of 5 replicates. Treatment I was negative control group without any treatment, treatment II were only infected with 103 T. evansi, treatment III, IV, and V were infected with 103 T. evansi and treated with willow tree bark extract with the dose of 30, 45, and 60 mg/kg bw, respectively. The administration of willow tree bark extract was carried out for 3 consecutive days orally using a stomach tube. All rats were sacrificed and necropsied then liver were collected for histopathological examination. Percentage of histophatological changes found in hepatocytes of rat infected with T. evansi after the administration of willow tree bark extract in group P1, P2, P3, P4, and P5 were: normal hepatocytes 96.28%; 0.00%; 8.06%; 40.61%; and 0.00%; hydropic degeneration 0.00%, 44.98%, 58.00%, 38.29%, and 35.04%; fatty degeneration 0.00%, 0.00%, 0.00%, 0.00%, and 8.76%; necrosis 3.72%, 55.02%, 33.94%, 21.10%, and 56.20, respectively. In conclusion, the administration of willow tree bark extract is able to protect rat’s liver from T. evansi infection and the dose of 45 mg/kg bw is more effective than 30 mg/kg bw but the dose of 60 mg/kg bw of willow tree bark extract worsen the liver damage.Key words: histopathology, liver, T. evansi, willow

INVESTIGATION OF THE EFFECT OF BLACK SEED OIL ON DIABETES-INDUCED - TESTICULAR DAMAGE: A HISTOPATHOLOGICAL STUDY

2021 ◽  
pp. 29-31
Author(s):  
Seval Kaya ◽  
Yusuf Nergiz ◽  
Firat Asir
Keyword(s):  
Tissue Damage ◽  
Seed Oil ◽  
Histopathological Examination ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Histopathological Study ◽  
Diabetes Group ◽  
Black Seed ◽  
Black Seed Oil

In this study, it was aimed to investigate the protective effect of black seed oil against testicular tissue damage in diabetic rats. A total of 18 male rats were divided into 3 groups, including 6 rats in each group.Groups; control (n=6), diabetes (n=6), diabetes + black seed oil (n=6). A single dose of 45 mg / kg streptozocine (STZ) was injected intraperitoneally to induce diabetes. Diabetes + Black seed oil group: For 56 days, 2.5 ml / kg of black seed oil was administered orally to rats.The rats were sacriced at the end of 56 days. Testicular tissues were taken for routine parafn tissue processing for histopathological examination. Parafn sections were stained with Hematoxylin-Eosin and PAS and examined under a light microscope. Atrophy and degeneration were observed in the seminiferous tubules of diabetic group. Histology of black seed oil group sections were similar to that of control group. A signicant difference was found between the black seed oil group and the diabetes group in terms of blood glucose values. As a result, we think that Black Seed Oil ameliorates to the tissue damage caused by diabetes and the decrease in blood sugar value.

scholarly journals EFFECT OF YOHIMBINE ON CLOMIPRAMINE-INDUCED SEXUAL DYSFUNCTION IN MALE RATS

2017 ◽  
Vol 10 (2) ◽  
pp. 92
Author(s):  
Devangam Sheshadri Shekar
Keyword(s):  
Sexual Behavior ◽  
Sexual Dysfunction ◽  
Histopathological Examination ◽  
Red Light ◽  
Female Rats ◽  
Male Rats ◽  
Male Rat ◽  
Testicular Damage

Object: The present investigation has been carried out to find out the effect of yohimbine on clomipramine-induced sexual dysfunction in male rats.Methods: The male rats were treated with clomipramine and yohimbine simultaneously for 60 days. During the treatment, all the male rats werechallenged with the female rats which are in estrous phase and their sexual behavior was observed under dim red light. Half of the animals in each group and remaining on 60 day were sacrificed, blood was collected and serum separated. Testis was collected and preserved in 10% formalin forsubsequent histopathological examination. thResults: The study reveals that yohimbine failed to antagonize the clomipramine-induced sexual dysfunction in male rats in all aspects, except thepartial improvement in the sexual behavior.Conclusion: Yohimbine a well-known aphrodisiac failed to antagonize the clomipramine-induced sexual dysfunction in male rats. The decrease intestosterone levels, a decrease in spermatozoa count were continued even in the presence of yohimbine except improvement in the sexual behaviorparameters. Hence, yohimbine could not be a safe antidote against clomipramine-induced sexual dysfunction in male rats.Keywords: Yohimbine, Clomipramine, Testosterone, Male rat sexual competence, Testicular damage.

scholarly journals Investigation Into Effect Of Crude Mixture Of Abrus Precatorius Seed On Hypothalamopituitary Gonadal Axis And Development Of Antifertility In Male Rats

1970 ◽  
Vol 36 (1) ◽  
pp. 103-109 ◽  
Author(s):  
S Talukder ◽  
MA Hossain ◽  
S Sarker ◽  
MAH Khan
Keyword(s):  
Negative Impact ◽  
Sperm Count ◽  
Sharp Decrease ◽  
Serum Levels ◽  
Serum Biochemistry ◽  
Male Rats ◽  
Male Rat ◽  
Control Group ◽  
Abrus Precatorius ◽  
Crude Mixture

To evaluate the antifertility effect of crude mixture of A. precatorius seeds at the dose level of 50 mg/kg body weight in adult male rats, after oral administration to male rats for 40 days, the rats were sacrificed and hormonal profiles, serum biochemistry, sperm count and histological changes were recorded. A sharp decrease in the serum levels of testosterone (0.70 ± 0.17 ng/ml), FSH (0.70 ± 0.22 lU/L), and LH (0.87 ± 0.35 IU/L) was detected compared to control (FSH, LH and testosterone levels 0.93 ± 0.15 ng/ml, 0.76 ± 0.28 IU/L, 1.44 ± .011 IU/L, respectively). A significant reduction of epididymal sperm count (2.34 million/mL) was noted in treated rats as compared to control group (7.87 million/mL). Histology of testes showed marked atrophy of the testes, which was characterized by disruption of the seminiferous epithelium and atrophy of the Leydig cells. Crude mixture of A. precatorius seed has a negative impact on male reproductive functions. It might be suggested that crude mixture of A. precatorius seeds might have antifertility property for male rats.   Keywords: Abrus precatorius; antifertility; male rat; testosterone. DOI: http://dx.doi.org/10.3329/bjar.v36i1.9234 BJAR 2011; 36(1): 103-109

scholarly journals The Effect of Experimental Escherichia coli Infection on Some Blood Parameters and Histological Changes in Male Rats

2011 ◽  
Vol 35 (2) ◽  
pp. 22-27
Author(s):  
Hayder AL – Zamely
Keyword(s):  
Escherichia Coli ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Histopathological Examination ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Infected Group ◽  
Male Rats ◽  
Control Group ◽  
Renal Tubules

This study was carried out to investigate the effect of experimental infection by Escherichia coli on some blood parameters and histopathological changes in male rats to reach this objective twenty male rats (Rattus norvigicus) were divided into two groups, housed under same conditions of water, feed, temperature, light and humidity. The first one served as control group, while the second was experimentally infected with Ecoli by administration of 2ml / rat of (1×109) colony forming unit/ml of E-coli. Five days later blood were obtained from animals by cardiac puncture to measure the total and differential white blood cells count hemoglobin concentration packed cells volume red blood cells count, and thrombocytes count. One animal from each group was killed and histopathological examination was done for intestine kidney liver and spleen to detect the effect of infection on these organs. The results revealed a significant increase in total leukocytes count (P<005) in infected group while the differential count showed a significant increase in neutrophils and significant decrease in lymphocytes (P< 0.05) in same group. There were a significant decrease in hemoglobin concentration packed cell volume and non significant decrease in red blood cells count. While thrombocytes count showed a significant decrease in infected group compared with control group (P<0.05). The histopathological examination of infected male rats intestine was demonstrated mild diffuse inflammation which include increased numbers of neutrophile in tissue intraepithelial lymphocytes and goblet cells In kidney renal tubules showed extensive epithelial swelling with decreased lumen space and generalized necrotic changes with interstitial hemorrhage in renal cortex. In liver hepatocytes were swollen with decreased sinusoidal spaces and widely distributed necrotic foci were seen. There is extensive splenic necrosis with infiltration of leukocytes from white pulp to red pulp of spleen.

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