scholarly journals Cellocity: A Python package for analysis of confluent cell layer dynamics

2021 ◽  
Vol 6 (57) ◽  
pp. 2818
Author(s):  
Jens Eriksson ◽  
Daniel Styrström ◽  
Mikael Sellin
Keyword(s):  
Cell Layer ◽  
Confluent Cell ◽  
Python Package

scholarly journals A simple nanoscale interface directs alignment of a confluent cell layer on oxide and polymer surfaces

2013 ◽  
Vol 1 (29) ◽  
pp. 3553 ◽  
Author(s):  
Patrick E. Donnelly ◽  
Casey M. Jones ◽  
Stephen B. Bandini ◽  
Shivani Singh ◽  
Jeffrey Schwartz ◽  
...  
Keyword(s):  
Cell Layer ◽  
Polymer Surfaces ◽  
Confluent Cell

Tissue Engineering of Fibroblast Constructs and Anisotropic Collagen Gels

2002 ◽  
Vol 724 ◽  
Author(s):  
Sarah Calve ◽  
Ellen Arruda ◽  
Robert Dennis ◽  
Karl Grosh ◽  
Krystyna Pasyk
Keyword(s):  
Tissue Engineering ◽  
Self Assembly ◽  
Cell Layer ◽  
Fetal Bovine Serum ◽  
Self Organization ◽  
Collagen Gels ◽  
Confluent Cell ◽  
Fetal Bovine

AbstractThe creation of an in vitro functional tendon construct will enable testing of the influence of mechanics and nutrients on the development and remodeling of tendon under known controlled stimuli which is difficult to achieve in vivo. Tendon constructs were engineered in vitrovia stress-mediated self organization of fibroblasts and ECM on a laminin coated elastomer substrate. Varying the laminin density and the amount of fetal bovine serum on the substrate affected the ability of tendon fibroblasts to form a confluent cell layer and the time to layer delamination. Understanding the factors that promote self-assembly of tendon constructs will enable their combination with already developed in vitro muscle constructs.

SEM of Hepatocytes and Biliary Passages in Goldfish Liver

1977 ◽  
Vol 35 ◽  
pp. 556-557
Author(s):  
Waykin Nopanitaya ◽  
Joe W. Grisham ◽  
Johnny L. Carson
Keyword(s):  
Carassius Auratus ◽  
Cell Layer ◽  
Three Dimensional ◽  
Cell Types ◽  
Biliary System ◽  
Fish Food ◽  
Commercial Fish ◽  
Goldfish Carassius Auratus ◽  
Commercial Fish Food

An interesting feature of the goldfish liver is the morphology of the hepatic plate, which is always formed by a two-cell layer of hepatocytes. Hepatic plates of the goldfish liver contain an infrequently seen second type of cell, in the centers of plates between two hepatocytes. A TEH study by Yamamoto (1) demonstrated ultrastructural differences between hepatocytes and centrally located cells in hepatic plates; the latter were classified as ductule cells of the biliary system. None of the previous studies clearly showed a three-dimensional organization of the two cell types described. In the present investigation we utilize SEM to elucidate the arrangement of hepatocytes and bile ductular cells in intralobular plates of goldfish liver.Livers from young goldfish (Carassius auratus), about 6-10 cm, fed commercial fish food were used for this study. Hepatic samples were fixed in 4% buffered paraformaldehyde, cut into pieces, fractured, osmicated, CPD, mounted Au-Pd coated, and viewed by SEM at 17-20 kV. Our observations were confined to the ultrastructure of biliary passages within intralobular plates, ductule cells, and hepatocytes.

Anchoring Fibrils in Arterial Endothelium

1969 ◽  
Vol 27 ◽  
pp. 274-275
Author(s):  
A. Trillo
Keyword(s):  
Carotid Arteries ◽  
Animal Species ◽  
Cell Layer ◽  
Present Communication ◽  
Internal Elastic Lamina ◽  
Endothelial Cell Layer ◽  
Structural Details ◽  
Rats And Mice ◽  
Arterial Endothelium ◽  
Elastic Lamina

There are conflicting reports regarding some fine structural details of arteries from several animal species. Buck denied the existence of a sub-endothelial space, while Karrer and Keech described a space of variable width which separates the endothelium from the underlying internal elastic lamina in aortas of aging rats and mice respectively.The present communication deals with the ultrastrueture of the interface between the endothelial cell layer and the internal elastic lamina as observed in carotid arteries from rabbits of varying ages.

Differentiation Processes of the Ocellar Retina of the Honeybee (Apis Mellifera L.)

1990 ◽  
Vol 48 (3) ◽  
pp. 430-431
Author(s):  
Maria Anna Pabst
Keyword(s):  
Apis Mellifera ◽  
Distal Part ◽  
Cell Layer ◽  
Single Layer ◽  
Photoreceptor Cells ◽  
Distal Segment ◽  
Compound Eyes ◽  
Thin Sections ◽  
Ultrastructural Studies ◽  
Adult Worker

In addition to the compound eyes, honeybees have three dorsal ocelli on the vertex of the head. Each ocellus has about 800 elongated photoreceptor cells. They are paired and the distal segment of each pair bears densely packed microvilli forming together a platelike fused rhabdom. Beneath a common cuticular lens a single layer of corneagenous cells is present.Ultrastructural studies were made of the retina of praepupae, different pupal stages and adult worker bees by thin sections and freeze-etch preparations. In praepupae the ocellar anlage consists of a conical group of epidermal cells that differentiate to photoreceptor cells, glial cells and corneagenous cells. Some photoreceptor cells are already paired and show disarrayed microvilli with circularly ordered filaments inside. In ocelli of 2-day-old pupae, when a retinogenous and a lentinogenous cell layer can be clearly distinguished, cell membranes of the distal part of two photoreceptor cells begin to interdigitate with each other and so start to form the definitive microvilli. At the beginning the microvilli often occupy the whole width of the developing rhabdom (Fig. 1).

1453: Lonidamine Reduces Prostate Weight in Rodents and is Accompanied by Apoptosis and Disruption of the Epithelial Cell Layer

2006 ◽  
Vol 175 (4S) ◽  
pp. 469-469
Author(s):  
W. Steve Ammons ◽  
Tatyana Lukoyanova ◽  
Amber Durnal ◽  
Geoffrey Del Rosario ◽  
Alan Colowick
Keyword(s):  
Epithelial Cell ◽  
Cell Layer ◽  
Prostate Weight ◽  
Epithelial Cell Layer

STUDIES ON OESTROGENS IN CATTLE

1960 ◽  
Vol XXXIV (I) ◽  
pp. 27-32 ◽  
Author(s):  
Stian Erichsen ◽  
Weiert Velle
Keyword(s):  
Bovine Liver ◽  
Liver Cells ◽  
Cell Sheets ◽  
Confluent Cell

ABSTRACT The metabolism of some oestrogenic hormones was studied in vitro by the use of cells grown on a medium free from blood. The methods used for the culture of cells from bovine testis, endometrium, amnion, and liver in confluent cell sheets on glass are described. The interconversion of oestrone and oestradiol-17β was demonstrated in the presence of cells from amnion, endometrium, and also from testicles of young calves and bulls. Only trace amounts of oestrone were found following incubations with oestradiol-17α in these tissues. Bovine liver cells grown in vitro showed a very poor capacity to bring about the interconversion mentioned above.

Sign in / Sign up

Export Citation Format

Share Document