scholarly journals Ion Exchange Properties of the Erythrocyte Surface Protein Band 3 and its Role in the Process of Oxygenation during CMV Infection in the Third Trimester of Pregnancy

2016 ◽  
Vol 6 (4) ◽  
pp. 271-275
Author(s):  
Michael Lucenko ◽  
◽  
Irina Andrievskaya ◽  
Artem Mironenko ◽  
◽  
...  
Keyword(s):  
Ion Exchange ◽  
Surface Protein ◽  
Protein Band ◽  
Band 3 ◽  
Cmv Infection ◽  
Third Trimester ◽  
The Third ◽  
Erythrocyte Surface ◽  
Exchange Properties

THE ROLE OF ESTRADIOL IN THE SUSTENTATION OF FETOPLACENTAL SYSTEM

2016 ◽  
Vol 1 (61) ◽  
pp. 75-79
Author(s):  
Андриевская ◽  
Irina Andrievskaya ◽  
Луценко ◽  
Mikhail Lutsenko ◽  
Довжикова ◽  
...  
Keyword(s):  
The State ◽  
Cmv Infection ◽  
Histochemical Reaction ◽  
Third Trimester ◽  
Paraffin Sections ◽  
Igg Antibody ◽  
The Third ◽  
Exchange Processes ◽  
Hormonal Dysfunction

The aim of the study is to define the role of abnormalities in the formation of estriol hormones in the development of hormonal dysfunction of fetoplacental system. There were examined 39 placentas from women with exacerbation of chronic cytomegalovirus infection at the third trimester of pregnancy and IgG antibody titer 1:1600 and 30 placentas from women with latent CMV infection and titer 1:400. The activity of androstenedione dehydrogenase was found out with histochemical method on cryostat sections of freshly frozen tissues of placentas by Lloyd’s method. The assessment of intensiveness of histochemical reaction was done with cytophotometric method by Scion program. Estradiol in homogenate of placenta was measured with immune-enzyme method. The quantity of nuclei of placenta syncytiotrophoblast in the state of apoptosis was done with cytophotometric method on paraffin sections stained by ISEL method. By the results of the study the exacerbation of CMV infection at the third trimester of pregnancy leads to the decrease of intensiveness of histochemical reaction in placentas to androstenedione dehydrogenase till 32.1±2.88 pixel/mcm2 (at the latent course it is 54.2±4.31 pixel/mcm2, р<0.001), and in homogenate of placenta to the decrease of estradiol till 18443.2±117.53 pmole/l (at the latent course of the disease it is 28977.7±158.13 pmole/l, p<0.001), on paraffin sections in syncytiotrophoblast by the increase of nuclei number in the state of apoptosis till 5.0±0.03% (at latent course of the disease it is 1.2±0.001%, р<0.001) and vacuole formation, which leads to destructive damages of syncytiotrophoblast cytoplasm. The obtained results prove that the exacerbation of CMV infection at the third trimester of pregnancy causes the decrease of estriol hormones formation, which is accompanied by the damage of morphostructure and exchange processes in cell elements of placenta.

CHANGES IN THE COMPOSITION OF LIPIDS IN PREGNANT WOMEN WITH CYTOMEGALOVIRUS INFECTION IN THE THIRD TRIMESTER OF PREGNANCY DEPENDING ON ANTIBODY TITERS OF IMMUNOGLOBULIN G

2016 ◽  
Vol 1 (61) ◽  
pp. 69-74 ◽  
Author(s):  
Ишутина ◽  
Nataliya Ishutina
Keyword(s):  
Lipid Metabolism ◽  
Phospholipase A2 ◽  
Pregnant Women ◽  
Peripheral Blood ◽  
Pathological Process ◽  
Study Group ◽  
Igg Antibodies ◽  
Cmv Infection ◽  
Third Trimester ◽  
The Third

The work was carried out to study the processes of lipid peroxidation, antioxidant defense, the content of phospholipase A2, the main structural phospholipids (phosphatidylethanolamine, phosphatidylcholine and its lizoform) in the peripheral blood of women with reactivation of chronic cytomegalovirus (CMV) infection in the III trimester of gestation depending on the titer of IgG antibodies to CMV. The method of random sample was used to select 90 pregnant women. The study group consisted of 60 pregnant women with exacerbation of chronic CMV infection in the third trimester. It was divided into two subgroups: subgroup A (30 women with active CMV infection, the titer of IgG antibodies to CMV was 1:1600); subgroup B (30 women with latent CMV infection, the titer of IgG antibodies to CMV was 1:800). The control group consisted of 30 healthy pregnant women who matched the study group by the period of gestation and age. It was found out that lipid metabolism imbalance in plasma and erythrocytes of the peripheral blood was one of the reasons for progression of the pathological process of CMV infection in pregnant women. Changes in the composition of erythrocyte membrane phospholipids, i.e the reduction of phosphatidylethanolamine by 22% (p<0.001), of phosphatidylcholine by 23% (p<0.001), an increase in the content of lysophosphatidylcholine by 34% (p<0.001), are regarded as membrane destabilizing processes. Their development is caused by uncontrolled free radical reactions leading to the initiation of peroxidation of cell membrane lipid oxidation (the increase in the concentration of TBA-active products by 78% (p<0.001), and the activation of lipolytic enzymes (increased concentration of phospholipase A2 by 60% (p<0.001). These changes occur against the oppression of antioxidant protection (reducing the concentration of α-tocopherol by 26% (p<0.001). The rate of change of lipid metabolism in these structures as manifestations of membrane destructive phenomena depends on the titer of IgG antibodies to CMV and is most pronounced when there is the active form of CMV infection (titer IgG antibodies to CMV is 1:1600). The mentioned pathological processes taken together aggravate the condition of pregnant women with CMV infection and create a favorable background for further progression of the pathological process, which greatly burdens the prenatal period and childbirth. The results obtained allow us to establish the important role of lipid metabolism disorders in the pathogenesis of CMV infection, which can be the basis for the expansion of the diagnostic and therapeutic base for this pathology of pregnant women.

scholarly journals THE PROCESSES OF LIPID PEROXIDATION IN THE ENDOTHELIUM OF THE ARTERIES OF THE UMBILICAL CORD IN CYTOMEGALOVIRUS INFECTION

2018 ◽  
Vol 1 (69) ◽  
pp. 59-63
Author(s):  
Николай Дорофиенко ◽  
Nikolay Dorofienko ◽  
Наталия Ишутина ◽  
Nataliya Ishutina ◽  
Ирина Андриевская ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Umbilical Artery ◽  
Lipid Peroxides ◽  
Control Group ◽  
Peroxidation Of Lipids ◽  
Cmv Infection ◽  
Third Trimester ◽  
Avidity Index ◽  
Morphological Examination ◽  
The Third

The purpose of the work is to study the processes of peroxidation of lipids and their influence on the structure of the endothelium of the umbilical artery of newborns from mothers who had experienced exacerbation of cytomegalovirus (CMV) infection in the third trimester of pregnancy. For the object of the study there were taken 45 umbilical cord samples during childbirth from women who had acute CMV infection in the third trimester of pregnancy (main group), and 25 umbilical cord samples from healthy women (control group). Clinical diagnosis of primary CMV infection was established by the presence of IgM antibodies to CMV and low-avid IgG in the peripheral blood (avidity index <50%), as well as CMV DNA detected by polymerase chain reaction in blood or urine; exacerbation of chronic CMV infection by the presence of IgM to CMV, high-avid IgG (avidity index >65%), and CMV DNA in scrapes taken from buccal epithelium. Lipid peroxides in the endothelium of the umbilical artery were detected by the Winkler-Schulze method. Morphological examination of the umbilical cord was performed by histochemical methods. The results of histochemical studies indicate that the exacerbation of CMV infection in the third trimester of pregnancy is associated with oxidative stress that causes the depletion of the protective systems and oxidant potential, which leads to the development of significant oxidative destruction of the endothelium of the umbilical cord artery of newborns. The revealed morphohistochemical changes in umbilical cord tissues in CMV infection can affect the functional state of this organ and be a morphological substrate for the appearance of its dysfunction, mainly the basis for the violation of fetoplacental blood circulation causing the development of hypoxia.

Correlations Between the Values of Maternal Glycemia from the Last Trimester of Pregnancy and Fetal Birth Weight

2013 ◽  
Vol 20 (3) ◽  
pp. 259-265
Author(s):  
Monica Vereş ◽  
Aurel Babeş ◽  
Szidonia Lacziko
Keyword(s):  
First Trimester ◽  
Mean Value ◽  
Fetal Macrosomia ◽  
Entire Group ◽  
Third Trimester ◽  
The Third ◽  
Group I ◽  
Fetal Birth Weight ◽  
First Trimester Of Pregnancy ◽  
The Mean

Abstract Background and aims: Gestational diabetes represents a form of diabetes diagnosed during pregnancy that is not clearly overt diabetes. In the last trimester of gestation the growth of fetoplacental unit takes place, thus maternal hyperglycemia will determine an increased transplacental passage, hyperinsulinemia and fetal macrosomia. The aim of our study was that o analyzing the effect of maternal glycemia from the last trimester of pregnancy over fetal weight. Material and method: We run an observational study on a group of 46 pregnant women taken into evidence from the first trimester of pregnancy, separated in two groups according to blood glucose determined in the third trimester (before birth): group I normoglycemic and group II with hyperglycemia (>92mg/dl). Results: The mean value of third trimester glycemia for the entire group was of 87.13±22.03. The mean value of the glycemia determined in the third trimester of pregnancy was higher in the second group (109.17 mg/dl) in comparison to the first group (74.,21 mg/dl). The ROC curve for third trimester glycemia as fetal macrosomia appreciation test has an AUC of 0.517. Conclusions: Glycemia determined in the last trimester of pregnancy cannot be used alone as the predictive factor for fetal macrosomia.

Features of the course and treatment of glaucoma during pregnancy

2020 ◽  
Vol 98 (3) ◽  
pp. 178-184
Author(s):  
T. V. Chernyakova ◽  
A. Yu. Brezhnev ◽  
I. R. Gazizova ◽  
A. V. Kuroyedov ◽  
A. V. Seleznev
Keyword(s):  
Pregnant Women ◽  
Laser Treatment ◽  
Progressive Disease ◽  
Clinical Course ◽  
The Other ◽  
Third Trimester ◽  
The Third ◽  
Chronic Progressive Disease ◽  
Hypotensive Therapy ◽  
Eye Pressure

In the review we have integrated all up-to-date knowledge concerning clinical course and treatment of glaucoma among pregnant women to help specialists choose a proper policy of treatment for such a complicated group of patients. Glaucoma is a chronic progressive disease. It rarely occurs among childbearing aged women. Nevertheless the probability to manage pregnant patients having glaucoma has been recently increasing. The situation is complicated by the fact that there are no recommendations on how to treat glaucoma among pregnant women. As we know, eye pressure is progressively going down from the first to the third trimester, so we often have to correct hypotensive therapy. Besides, it is necessary to take into account the effect of applied medicines on mother health and evaluate possible teratogenic complications for a fetus. The only medicine against glaucoma which belongs to category B according to FDA classification is brimonidine. Medicines of the other groups should be prescribed with care. Laser treatment or surgery may also be a relevant decision when monitoring patients who are planning pregnancy or just bearing a child. Such treatment should be also accompanied by medicines.

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