scholarly journals A Study of HIV-1 Genetic Diversity in the Czech Republic: 1986-2007

2008 ◽  
Vol 16 (4) ◽  
pp. 175-177 ◽  
Author(s):  
Marek Linka ◽  
Marie Brůčková ◽  
Marek Malý ◽  
Jana Vandasová ◽  
Marie Staňková ◽  
...  
2011 ◽  
Vol 51 (No. 4) ◽  
pp. 151-155 ◽  
Author(s):  
T. Vyhnánek ◽  
J. Bednář

 Genetic diversity was detected in 11 varieties of triticale registered in the Czech Republic by means of polymorphism of prolamin proteins using the PAGE ISTA method. The polymorphism of prolamin proteins allowed the differentiation of the individual triticale varieties in 2002 and 2003 harvests. On the basis of Dice’s calculations of coefficients of similarity we discovered, in parallel with the uniform genotypes, genotypes with sister prolamin spectrums with a different percentage of participation in the respective years. A uniform spectrum was detected in the following varieties: Disco, Kolor, Lamberto, Marko, Presto, Sekundo, Ticino and Tricolor; Kitaro and Modus were dimorphous varieties. In 2003 three sister prolamin lines appeared in the variety Gabo and in 2004 only two. In 2003 a 5% admixture of a foreign genotype was detected in the variety Marko. Typical of the unknown genotype was the gliadin block Gld 1B3, which is the marker of rye translocation T1BL.1RS, gene Sr31 with resistance to black rust, higher cold resistance and lower baking quality of the wheat. The prolamin proteins of triticale grain are suitable for the detection of the genetic diversity and for the assessment of varietal authenticity and purity in seed samples of triticale varieties registered in the Czech Republic.


Aquaculture ◽  
2010 ◽  
Vol 298 (3-4) ◽  
pp. 194-201 ◽  
Author(s):  
Martin Hulak ◽  
Vojtech Kaspar ◽  
Klaus Kohlmann ◽  
Kevin Coward ◽  
Jakub Tešitel ◽  
...  

2011 ◽  
Vol 48 (No. 8) ◽  
pp. 343-350 ◽  
Author(s):  
J. Patzak

In the last year, new hybrid hop (Humulus lupulus L.) variety Agnus was released for cultivation in the Czech Republic. It has been necessary to prepare the quality system of Agnus identification from other Czech genotypes and characterise the germplasm of this variety by molecular methods. We proved that utilization of five STS primer combinations successfully and completely identified and determined Czech released varieties and new promising breeding materials. The use of STS method was also very effective and sensitive for control of authenticity and purity of variety Agnus in multipropagation cycle. The study of genetic diversity of 61 hop varieties by RAPD, STS, ISSR and AFLP methods confirmed, that germplasm of variety Agnus has ranked among high-alfa varieties. The results can be successfully used for identification, germplasm management, genetic studies and breeding purposes by breeders, multipropagators and hop growers.


2015 ◽  
Vol 114 (4) ◽  
pp. 1619-1624 ◽  
Author(s):  
Pavla Wagnerová ◽  
Bohumil Sak ◽  
John McEvoy ◽  
Michael Rost ◽  
Agniezska Perec Matysiak ◽  
...  

Author(s):  
Jiří Bezdíček

This thesis was meant to evaluate allele and genotype frequencies of kappa-casein (CSN3) in artificial insemination bulls in the Czech Republic of Czech Fleckvieh breed (n = 133) and Holstein breed (n = 58). Studied bulls were born between 1990 and 2005. Calculation was made by PowerMarker (Liu K., Muse S.V. 2005). Genotype frequencies established in Czech Fleckvieh bulls were AA = 0.391 AB = 0.4887 AE = 0.0075 BB = 0.0902 BE = 0.0226 and EE = 0 and allele frequencies were A = 0.6391 B = 0.3459 E = 0.015. Genotype frequencies established in Holstein bulls were AA = 0.5517 AB = 0.2759 AE = 0.1207 BB = 0 BE = 0.0345 and EE = 0.0172 and allele frequencies A = 0.75 B = 0.1552 and E = 0.0948. In Holstein breed we found higher frequency of E allele and higher frequency of genotypes with this allele. Genetic diversity, heterozygosis and PIC values in Czech Fleckvieh Bulls were following 0.4717; 0.5188 and 0.3737 and 0.4044; 0.4310 and 0.3668 in Holstein Bulls. These results show the higher genetic variability in bulls of Czech Fleckvieh breed. Results of χ2 test (Czech Fleckvieh 4.58; Holstein 2.55) show genetical disequilibrium in the Bulls both examined breeds used in artificial insemination in the Czech Republic.


Virus Genes ◽  
2009 ◽  
Vol 38 (3) ◽  
pp. 468-474 ◽  
Author(s):  
J. K. Kundu ◽  
S. Gadiou ◽  
G. Červená

2020 ◽  
Vol 73 (1) ◽  
pp. 64-76
Author(s):  
Dagmar Zádrapová ◽  
Jiří Korecký ◽  
Jakub Dvořák ◽  
Zuzana Faltinová ◽  
Jan Bílý

Abstract European beech (Fagus sylvatica L.) is one of the most important broadleaved tree species in Europe both ecologically and economically. Nowadays, in the Czech Republic, beech is underrepresented in forest tree species composition, and there are tendencies to increase its proportion. When reintroducing beech, genetic variability, along with other factors, play a key role. The main aim of this study was to evaluate the genetic diversity of ten selected indigenous beech populations across the Czech Republic. Two hundred and fifty individuals were genotyped on 21 polymorphic nuclear microsatellite markers, which were amplified using two newly assembled multiplexes. According to the results, observed heterozygosity (Ho ) among populations ranged from 0.595 to 0.654 and expected heterozygosity (He ) from 0.650 to 0.678. That is comparable with the findings in other European studies. The high discriminatory power of the assembled multiplexes was confirmed by calculating the Probability of Identity among both unrelated and related individuals. Principal Coordinate Analysis (PCoA) based on Nei's genetic distances revealed that there are genetic differences among populations resulting in three approximate clusters (geographically north, south-east, and south-west). Nevertheless, the results implicate that on a geographical scale of the Czech Republic, the distance is unlikely to be the primary driver of genetic differentiation.


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