scholarly journals Direct Ovarian Effects of a GnRH Agonist in Hypophysectomized Immature Rats: Inhibition of Theca-Interstitial Luteinizing Hormone Receptor mRNA Expression and Induction of Interstitial Cell Differentiation

2000 ◽  
Vol 17 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Hiroshi Kogo ◽  
Akiko Iizuka-Kogo ◽  
Toyoshi Fujimoto ◽  
Takao Mori
Keyword(s):  
Cell Differentiation ◽  
Luteinizing Hormone ◽  
Mrna Expression ◽  
Gnrh Agonist ◽  
Hormone Receptor ◽  
Interstitial Cell ◽  
Luteinizing Hormone Receptor ◽  
Receptor Mrna ◽  
Immature Rats

Decreased Luteinizing Hormone Receptor mRNA Expression in Human Ovarian Epithelial Cancer

2000 ◽  
Vol 79 (2) ◽  
pp. 158-168 ◽  
Author(s):  
Jean J. Lu ◽  
Yu Zheng ◽  
Xin Kang ◽  
Jian-Min Yuan ◽  
Stuart C. Lauchlan ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Mrna Expression ◽  
Hormone Receptor ◽  
Luteinizing Hormone Receptor ◽  
Epithelial Cancer ◽  
Receptor Mrna ◽  
Ovarian Epithelial Cancer

scholarly journals Association of luteinizing hormone receptor gene expression with cell cycle progression in granulosa cells

2009 ◽  
Vol 296 (6) ◽  
pp. E1392-E1399 ◽  
Author(s):  
Jennifer D. Cannon ◽  
Srinivas V. Seekallu ◽  
Catherine A. VandeVoort ◽  
Charles L. Chaffin
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Luteinizing Hormone ◽  
Mrna Expression ◽  
Granulosa Cells ◽  
Hormone Receptor ◽  
Cell Cycle Progression ◽  
Luteinizing Hormone Receptor ◽  
Cycle Progression ◽  
Immature Rats

During hormonally induced ovarian follicle growth, granulosa cell proliferation increases and returns to baseline prior to the administration of an ovulatory stimulus. Several key genes appear to follow a similar pattern, including the luteinizing hormone receptor (LHCGR), suggesting an association between cell cycle progression and gene expression. The expression of LHCGR mRNA in granulosa cells isolated from immature rats and treated in culture with FSH increased in a time-dependent manner, whereas administration of the cell cycle inhibitor mimosine completely suppressed expression. Although forskolin was able to induce luteinization in cells treated with mimosine, human chorionic gonadotropin had no effect, indicating the functional loss of LHCGR. The effects of mimosine on cell cycle progression and LHCGR mRNA expression were reversible within 24 h of mimosine removal. Cell cycle inhibition did not alter the stability of LHCGR mRNA, indicating that the primary effect was at the transcriptional level. To determine whether the relationship between LHCGR expression and cell cycle were relevant in vivo, immature rats were given a bolus of PMSG, followed by a second injection of either saline or PMSG 24 h later to augment levels of proliferation. The expression of LHCGR mRNA was elevated in the ovaries of animals receiving a supplement of PMSG. Mimosine also blocked cell cycle progression and LHCGR mRNA expression in macaque granulosa cells isolated following controlled ovarian stimulation cycles and in two different mouse Leydig tumor lines. These data collectively indicate that LHCGR mRNA is expressed as a function of the passage of cells across the G1-S phase boundary.

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