Identification and Expression Analysis of Upregulated Genes in the Resting Egg-Producing Water Flea (Daphnia pulex)

2016 ◽  
Vol 33 (1) ◽  
pp. 106
Author(s):  
Tomoko Takahashi ◽  
Masaaki Ohnuma
Keyword(s):  
Expression Analysis ◽  
Daphnia Pulex ◽  
Water Flea ◽  
Resting Egg ◽  
Upregulated Genes

scholarly journals Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils

Viruses ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 46 ◽  
Author(s):  
Yi-Sheng Sun ◽  
Zhang-Nv Yang ◽  
Fang Xu ◽  
Chen Chen ◽  
Hang-Jing Lu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Enterovirus 71 ◽  
Foot And Mouth Disease ◽  
Global Gene Expression ◽  
Solid Foundation ◽  
Differential Gene ◽  
Global Gene Expression Analysis ◽  
Upregulated Genes

Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are the two most important pathogens of hand, foot, and mouth disease (HFMD). However, the neuropathogenesis of EV71 and CVA16 has not been elucidated. In our previous study, we established gerbils as a useful model for both EV71 and CVA16 infection. In this work, we used RNA-seq technology to analyze the global gene expression of the brainstem of EV71- and CVA16-infected gerbils. We found that 3434 genes were upregulated while 916 genes were downregulated in EV71-infected gerbils. In CVA16-infected gerbils, 1039 genes were upregulated, and 299 genes were downregulated. We also found significant dysregulation of cytokines, such as IP-10 and CXCL9, in the brainstem of gerbils. The expression levels of 10 of the most upregulated genes were confirmed by real-time RT-PCR, and the upregulated tendency of most genes was in accordance with the differential gene expression (DGE) results. Our work provided global gene expression analysis of virus-infected gerbils and laid a solid foundation for elucidating the neuropathogenesis mechanisms of EV71 and CVA16.

The water flea Daphnia pulex (Cladocera, Daphniidae), a possible model organism to evaluate aspects of freshwater ecosystems

Crustaceana ◽  
2019 ◽  
Vol 92 (11-12) ◽  
pp. 1415-1426
Author(s):  
Juan-Alejandro Norambuena ◽  
Jorge Farías ◽  
Patricio De los Ríos
Keyword(s):  
Phylogenetic Trees ◽  
Daphnia Pulex ◽  
Model Organism ◽  
Freshwater Ecosystems ◽  
Intraspecific Diversity ◽  
Molecular Tools ◽  
Water Flea ◽  
Future Studies ◽  
Molecular Studies

Abstract Daphnia pulex is a freshwater planktonic crustacean, allegedly a cosmopolitan species, which is found in lentic ecosystems. The aim of this study was to conduct a literature review of D. pulex related to its life history and genetic variability, in order to mark a route for future studies. We noted that D. pulex is a model species on which ecological studies have been carried out, as well as molecular studies, in which its molecular diversity has been characterized and such in specimens from different environments: both pristine and under human influence. In particular those studies are highlighted, in which molecular tools have been used to construct phylogenetic trees for study intraspecific differences. Also, in some of these molecular studies, analyses of genetic, inter- and intraspecific diversity have been performed. In addition, analyses of protein expression in D. pulex and related species seem promising in evaluating the detailed role of this species.

scholarly journals Development of a microinjection system for RNA interference in the water flea Daphnia pulex

2013 ◽  
Vol 13 (1) ◽  
pp. 96 ◽  
Author(s):  
Chizue Hiruta ◽  
Kenji Toyota ◽  
Hitoshi Miyakawa ◽  
Yukiko Ogino ◽  
Shinichi Miyagawa ◽  
...  
Keyword(s):  
Rna Interference ◽  
Daphnia Pulex ◽  
Water Flea

Expression analysis of a transformer gene in Daphnia pulex after RNAi

2016 ◽  
Vol 50 (5) ◽  
pp. 748-754 ◽  
Author(s):  
C. Y. Guo ◽  
P. Chen ◽  
M. M. Zhang ◽  
J. J. Ning ◽  
C. L. Wang ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Daphnia Pulex

scholarly journals Gene up-regulation in response to predator kairomones in the water flea, Daphnia pulex

2010 ◽  
Vol 10 (1) ◽  
pp. 45 ◽  
Author(s):  
Hitoshi Miyakawa ◽  
Maki Imai ◽  
Naoki Sugimoto ◽  
Yuki Ishikawa ◽  
Asano Ishikawa ◽  
...  
Keyword(s):  
Daphnia Pulex ◽  
Water Flea

Photoperiodism of Male Offspring Production in the Water Flea Daphnia pulex

2017 ◽  
Vol 34 (4) ◽  
pp. 312 ◽  
Author(s):  
Kenji Toyota ◽  
Tomomi Sato ◽  
Norihisa Tatarazako ◽  
Taisen Iguchi
Keyword(s):  
Daphnia Pulex ◽  
Male Offspring ◽  
Water Flea ◽  
Offspring Production

Cloning and expression analysis of a transformer gene in Daphnia pulex during different reproduction stages

2014 ◽  
Vol 146 (3-4) ◽  
pp. 227-237 ◽  
Author(s):  
Ping Chen ◽  
Shan-Liang Xu ◽  
Wei Zhou ◽  
Xiao-Ge Guo ◽  
Chun-Lin Wang ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Daphnia Pulex ◽  
Cloning And Expression

scholarly journals Metabolomics reveals an involvement of pantothenate for male production responding to the short-day stimulus in the water flea, Daphnia pulex

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Kenji Toyota ◽  
Alex Gavin ◽  
Shinichi Miyagawa ◽  
Mark R. Viant ◽  
Taisen Iguchi
Keyword(s):  
Daphnia Pulex ◽  
Male Production ◽  
Water Flea ◽  
Short Day

scholarly journals Erratum to: “Expression analysis of a transformer gene in Daphnia pulex after RNAi”

2017 ◽  
Vol 51 (2) ◽  
pp. 339-339
Author(s):  
C. Y. Guo ◽  
P. Chen ◽  
M. M. Zhang ◽  
J. J. Ning ◽  
C. L. Wang ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Daphnia Pulex
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