scholarly journals Assessing the Aquatic Toxicity of Insensitive Munitions (IM) Compounds Using 10-Day Aqueous Exposures with the Amphipod Hyalella Azteca : Scientific Operating Procedure Series : Characterization of IMX Ecotoxicological Effects

2018 ◽  
Author(s):  
Guilherme Lotufo ◽  
Mark Ballentine ◽  
Jacob Stanley
Keyword(s):  
Operating Procedure ◽  
Aquatic Toxicity ◽  
Hyalella Azteca ◽  
Ecotoxicological Effects ◽  
Insensitive Munitions

scholarly journals Evidence for Phytoremediation and Phytoexcretion of NTO from Industrial Wastewater by Vetiver Grass

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 74
Author(s):  
Abhishek RoyChowdhury ◽  
Pallabi Mukherjee ◽  
Saumik Panja ◽  
Rupali Datta ◽  
Christos Christodoulatos ◽  
...  
Keyword(s):  
Stress Reduction ◽  
Industrial Wastewater ◽  
Plant Biomass ◽  
Ionization Mass ◽  
Vetiver Grass ◽  
Insensitive Munitions ◽  
Treatment Technologies ◽  
And Control ◽  
Near Future

The use of insensitive munitions such as 3-nitro-1,2,4-triazol-5-one (NTO) is rapidly increasing and is expected to replace conventional munitions in the near future. Various NTO treatment technologies are being developed for the treatment of wastewater from industrial munition facilities. This is the first study to explore the potential phytoremediation of industrial NTO-wastewater using vetiver grass (Chrysopogon zizanioides L.). Here, we present evidence that vetiver can effectively remove NTO from wastewater, and also translocated NTO from root to shoot. NTO was phytotoxic and resulted in a loss of plant biomass and chlorophyll. The metabolomic analysis showed significant differences between treated and control samples, with the upregulation of specific pathways such as glycerophosphate metabolism and amino acid metabolism, providing a glimpse into the stress alleviation strategy of vetiver. One of the mechanisms of NTO stress reduction was the excretion of solid crystals. Scanning electron microscopy (SEM), electrospray ionization mass spectrometry (ESI-MS), and Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the presence of NTO crystals in the plant exudates. Further characterization of the exudates is in progress to ascertain the purity of these crystals, and if vetiver could be used for phytomining NTO from industrial wastewater.

scholarly journals Characterization of planktonic and biofilm fatty acid profiles and evaluation of their trophic transfer potential to Hyalella Azteca

2021 ◽  
Author(s):  
Jerry Chien-Yao Chao
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Bacterial Species ◽  
Saturated Fatty Acids ◽  
Hyalella Azteca ◽  
Fatty Acid Profiles ◽  
Fa Composition ◽  
Branched Chain ◽  
Transfer Potential

Fatty acid (FA) composition between biofilms and batch planktonic cultures were compared for two bacterial species Pseudomonas aeruginosa and Staphylococcus aureaus. Biofilm cultures exhibited decrease in saturated fatty acids (SAFA) that potentially conform to a more fluidic biophysical membrane property. The amount of FA in the biofilms' extracellular polymeric substance was not sufficient to consider it having a major contribution to the observed differences between biofilms and batch planktonic cultures. While biofilm grazing by the amphipod Hyalella azteca was evident, only certain bacteria-specific FA appeared to have the potential to be retained (odd-number SAFA and branched-chain FA). H. azteca with diet strictly consisted of bacteria biofilms did not demonstrate significant changes in their nutritional condition in terms of ω-3 and ω-6 polyunsaturated fatty acids (PUFA): combined with the results from fasting trials, H. azteca appears to have the capacity to retain ω-3 and ω-6 PUFAs up to 10 days.

Dissolution kinetics of IMX-101 and IMX 104 : Operating Procedure series : characterization of IMX dissolution

2016 ◽  
Author(s):  
Christian McGrath ◽  
Beth Porter ◽  
Jonathon Brame ◽  
Mark Chappell ◽  
Amber Russell
Keyword(s):  
Operating Procedure ◽  
Dissolution Kinetics ◽  
Kinetics Of

Subchronic, chronic, lethal and sublethal toxicity of insensitive munitions mixture formulations relative to individual constituents in Hyalella azteca

Chemosphere ◽  
2018 ◽  
Vol 210 ◽  
pp. 795-804 ◽  
Author(s):  
Guilherme R. Lotufo ◽  
Jacob K. Stanley ◽  
Pornsawan Chappell ◽  
Nicolas L. Melby ◽  
Mitchell S. Wilbanks ◽  
...  
Keyword(s):  
Hyalella Azteca ◽  
Sublethal Toxicity ◽  
Insensitive Munitions
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