scholarly journals Successful Identification of Pathogens by Polymerase Chain Reaction (PCR)-Based Electron Spray Ionization Time-of-Flight Mass Spectrometry (ESI-TOF-MS) in Culture-Negative Periprosthetic Joint Infection

2012 ◽  
Vol 94 (24) ◽  
pp. 2247-2254 ◽  
Author(s):  
Christina L Jacovides ◽  
Rachael Kreft ◽  
Bahar Adeli ◽  
Bryan Hozack ◽  
Garth D Ehrlich ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Polymerase Chain Reaction ◽  
Periprosthetic Joint Infection ◽  
Joint Infection ◽  
Chain Reaction ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Polymerase Chain ◽  
Culture Negative ◽  
Tof Ms

Commercially Available Polymerase Chain Reaction Has Minimal Utility in the Diagnosis of Periprosthetic Joint Infection

Orthopedics ◽  
2020 ◽  
Vol 43 (6) ◽  
pp. 333-338
Author(s):  
Beau J. Kildow ◽  
Sean P. Ryan ◽  
Richard Danilkowicz ◽  
Alexander L. Lazarides ◽  
Tyler J. Vovos ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Periprosthetic Joint Infection ◽  
Joint Infection ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Higher sensitivity of swab polymerase chain reaction compared with tissue cultures for diagnosing periprosthetic joint infection

2018 ◽  
Vol 26 (1) ◽  
pp. 230949901876529 ◽  
Author(s):  
Mohamed Omar ◽  
Maximilian Petri ◽  
Nael Hawi ◽  
Christian Krettek ◽  
Jörg Eberhard ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Periprosthetic Joint Infection ◽  
Joint Infection ◽  
Tissue Cultures ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Higher Sensitivity

scholarly journals Rapid detection of periprosthetic joint infection using a combination of 16s rDNA polymerase chain reaction and lateral flow immunoassay

2018 ◽  
Vol 7 (1) ◽  
pp. 12-19 ◽  
Author(s):  
V. Janz ◽  
J. Schoon ◽  
C. Morgenstern ◽  
B. Preininger ◽  
S. Reinke ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Synovial Fluid ◽  
16S Rdna ◽  
Periprosthetic Joint Infection ◽  
Bacterial Species ◽  
Joint Infection ◽  
Lateral Flow Immunoassay ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Proof Of Principle

Objectives The objective of this study was to develop a test for the rapid (within 25 minutes) intraoperative detection of bacteria from synovial fluid to diagnose periprosthetic joint infection (PJI). Methods The 16s rDNA test combines a polymerase chain reaction (PCR) for amplification of 16s rDNA with a lateral flow immunoassay in one fully automated system. The synovial fluid of 77 patients undergoing joint aspiration or primary or revision total hip or knee surgery was prospectively collected. The cohort was divided into a proof-of-principle cohort (n = 17) and a validation cohort (n = 60). Using the proof-of-principle cohort, an optimal cut-off for the discrimination between PJI and non-PJI samples was determined. PJI was defined as detection of the same bacterial species in a minimum of two microbiological samples, positive histology, and presence of a sinus tract or intra-articular pus. Results The 16s rDNA test proved to be very robust and was able to provide a result in 97% of all samples within 25 minutes. The 16s rDNA test was able to diagnose PJI with a sensitivity of 87.5% and 82%, and a specificity of 100% and 89%, in the proof-of-principle and validation cohorts, respectively. The microbiological culture of synovial fluid achieved a sensitivity of 80% and a specificity of 93% in the validation cohort. Conclusion The 16s rDNA test offers reliable intraoperative detection of all bacterial species within 25 minutes with a sensitivity and specificity comparable with those of conventional microbiological culture of synovial fluid for the detection of PJI. The 16s rDNA test performance is independent of possible blood contamination, culture time and bacterial species. Cite this article: V. Janz, J. Schoon, C. Morgenstern, B. Preininger, S. Reinke, G. Duda, A. Breitbach, C. F. Perka, S. Geissler. Rapid detection of periprosthetic joint infection using a combination of 16s rDNA polymerase chain reaction and lateral flow immunoassay: A Pilot Study. Bone Joint Res 2018;7:12–19. DOI: 10.1302/2046-3758.71.BJR-2017-0103.R2.

A new multiplex real-time polymerase chain reaction assay for the diagnosis of periprosthetic joint infection

2017 ◽  
Vol 27 (6) ◽  
pp. 1072-1078 ◽  
Author(s):  
Masaki Kawamura ◽  
Naomi Kobayashi ◽  
Yutaka Inaba ◽  
Hyonmin Choe ◽  
Taro Tezuka ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Periprosthetic Joint Infection ◽  
Polymerase Chain Reaction Assay ◽  
Joint Infection ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Detecção de microrganismos em dispositivos ortopédicos sonicados clínicos usando cultura convencional e qPCR

2021 ◽  
Author(s):  
Victoria Stadler Tasca Ribeiro ◽  
Juliette Cieslinski ◽  
Julia Bertol ◽  
Ana Laura Schumacher ◽  
João Paulo Telles ◽  
...  
Keyword(s):  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Chain Reaction ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Polymerase Chain ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Resumo Objetivo Avaliar a sensibilidade e a especificidade da reação em cadeia de polimerase em tempo real quantitativa (quantitative real-time polymerase chain reaction, qPCR, em inglês) para a triagem do gene rDNA 16S, com a utilização do fluido sonicado de implantes ortopédicos. Métodos Um estudo retrospectivo foi realizado em 73 fluidos sonicados obtidos de pacientes com infecção associada aos implantes ortopédicos. As amostras foram submetidas a cultura convencional e a teste molecular utilizando ionização e dessorção a laser assistida por matriz com espectrometria de massa por tempo de voo (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF MS, em inglês) e qPCR para o gene rDNA 16S. Os valores limiares do ciclo foram usados para definir um ponto de corte para a qPCR do gene rDNA 16S para culturas negativas e positivas. Resultados Não foram observadas diferenças estatísticas entre os grupos de cultura positiva e negativa com base no tempo desde a primeira cirurgia até a infecção (p = 0,958), na idade (p = 0,269), ou nas comorbidades em geral. No entanto, uma diferença estatística foi encontrada entre a duração média do uso de antibióticos antes da remoção do dispositivo (3,41 versus 0,94; p = 0,016). O DNA bacteriano foi identificado em todas as amostras dos fluidos sonicados. Os limiares do ciclo médio de culturas positivas e negativas foram de 25,6 e 27,3, respectivamente (p < 0,001). Como uma ferramenta de diagnóstico, um corte do limite do ciclo de 26,89 demonstrou uma área sob a curva da característica de operação do receptor de 0,877 (p ≤ 0,001). Conclusão A presença de agentes antimicrobianos por mais de 72 horas diminuiu a positividade da cultura, mas não influenciou os resultados da qPCR. Apesar disso, a amplificação do rDNA 16S pode sobrestimar o diagnóstico de infecção.

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