scholarly journals Detection of Periprosthetic Infections With Use of Ribosomal RNA-Based Polymerase Chain Reaction

2010 ◽  
Vol 92 (3) ◽  
pp. 654-663 ◽  
Author(s):  
Patrick F Bergin ◽  
Jason D Doppelt ◽  
William G Hamilton ◽  
Gudrun E Mirick ◽  
Angela E Jones ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Ribosomal Rna ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Periprosthetic Infections

scholarly journals Clinical Evaluation of a 16S Ribosomal RNA Polymerase Chain Reaction Test for the Diagnosis of Lymph Node Tuberculosis

2006 ◽  
Vol 43 (7) ◽  
pp. 855-859 ◽  
Author(s):  
Fernando Osores ◽  
Oscar Nolasco ◽  
Kristien Verdonck ◽  
Jorge Arevalo ◽  
Juan Carlos Ferrufino ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Lymph Node ◽  
Rna Polymerase ◽  
Ribosomal Rna ◽  
Polymerase Chain Reaction Test ◽  
Chain Reaction ◽  
Reaction Test ◽  
Polymerase Chain ◽  
Lymph Node Tuberculosis ◽  
Chain Reaction Test

scholarly journals 1834. Incremental Diagnostic Value of 16S Ribosomal RNA Gene Polymerase Chain Reaction/Sanger Sequencing in Clinical Practice

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S44-S44
Author(s):  
Sarwat Khalil ◽  
Madiha Fida ◽  
Douglas W Challener ◽  
Omar Abu Saleh ◽  
Muhammad R Sohail ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Practice ◽  
16S Rrna ◽  
Ribosomal Rna ◽  
Mayo Clinic ◽  
Sanger Sequencing ◽  
Diagnostic Value ◽  
Chain Reaction ◽  
Material Support ◽  
Polymerase Chain

Abstract Background Polymerase chain reaction (PCR)/sequencing targeting the 16S ribosomal RNA (rRNA) gene to detect bacteria in normally sterile tissues and fluids has become increasingly popular in clinical medicine. This culture-independent technique can detect bacteria that are nonviable or difficult to cultivate using conventional methods. The clinical value of this type of testing is not well defined. We aimed to assess the diagnostic value of 16S rRNA PCR/Sanger sequencing as a clinical diagnostic assay at Mayo Clinic. Methods This is an interim analysis of the first 173 of 478 patients who had 16S rRNA PCR/Sanger sequencing done on sterile tissues or fluids at our institution from April, 2017 to November, 2018 as part of routine clinical practice. Medical records are being retrospectively reviewed, with results compared with those of culture. Results We reviewed 207 specimens from 173 patients (musculoskeletal 79%, cardiovascular 7%, central nervous system 4%, other 9%) that underwent 16S rRNA PCR/Sanger sequencing by clinical request (Table 1). In 90% of these specimens, the test was pre-planned rather than added-on. Nine specimens were excluded from analysis, as cultures were not performed. Overall concordance of culture with PCR/sequencing was 81% (160/197; P < 0.0001). Of 44 culture-positive specimens, PCR detected the same bacterium in 21 (48%) (Table 2). 45% (20/44) of those with positive cultures and 46% of those with positive PCR/sequencing results had received prior antimicrobial therapy (Table 3). PCR was negative in 139/144 specimens that were culture-negative (97%). PCR/sequencing was helpful in detecting a putative bacterial pathogen in 4 patients with negative cultures (Table 4). Conclusion Overall, 16S rRNA PCR/Sanger sequencing improved diagnostic yield compared with culture in a minority of cases. The described assay is limited by its inability to detect polymicrobial infections, a technical limitation that could possibly be addressed using massive parallel sequencing. Careful selection of cases and a save and add-on approach may be more cost-effective than upfront testing, although this was requested in a minority of cases. Disclosures Robin Patel, MD, ASM and IDSA: Other Financial or Material Support, Travel reimbursement, editor’s stipends; CD Diagnostics, Merck, Hutchison Biofilm Medical Solutions, Accelerate Diagnostics, ContraFect, TenNor Therapeutics Limited, Shionogi: Grant/Research Support; Curetis, Specific Technologies, NextGen Diagnostics, PathoQuest, Qvella: Consultant; NBME, Up-to-Date, the Infectious Diseases Board Review Course: Honorarium recipient, Other Financial or Material Support; Patent on Bordetella pertussis/parapertussis PCR issued, a patent on a device/method for sonication with royalties paid by Samsung to Mayo Clinic, and a patent on an anti-biofilm substance issued: Other Financial or Material Support, Patents.

Fecal Polymerase Chain Reaction with 16S Ribosomal RNA Primers Can Detect the Presence of Gastrointestinal Helicobacter in Dogs

2002 ◽  
Vol 16 (4) ◽  
pp. 426 ◽  
Author(s):  
Joanne K. Shinozaki ◽  
Rance K. Sellon ◽  
Glenn H. Cantor ◽  
Tom E. Besser ◽  
Katrina L. Mealey ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Ribosomal Rna ◽  
16S Ribosomal Rna ◽  
Chain Reaction ◽  
Polymerase Chain
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