scholarly journals A Call for Standardization in Cell Therapy Studies

2019 ◽  
Vol 101 (10) ◽  
pp. e47
Author(s):  
Scott A. Rodeo
Keyword(s):  
Cell Therapy ◽  
Therapy Studies

scholarly journals Modeling chronic cervical spinal cord injury in aged rats for cell therapy studies

2021 ◽  
Vol 94 ◽  
pp. 76-85
Author(s):  
María Martín-López ◽  
Elena González-Muñoz ◽  
Emilio Gómez-González ◽  
Rosario Sánchez-Pernaute ◽  
Javier Márquez-Rivas ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Cell Therapy ◽  
Cervical Spinal Cord ◽  
Cervical Spinal Cord Injury ◽  
Aged Rats ◽  
Cord Injury ◽  
Therapy Studies

scholarly journals Trends in clinical trials for stroke by cell therapy: data mining ClinicalTrials.gov and the ICTRP portal site

2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Takaharu Negoro ◽  
Hanayuki Okura ◽  
Midori Maehata ◽  
Shigekazu Hayashi ◽  
Satoru Yoshida ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Cell Therapy ◽  
Chronic Phase ◽  
Cell Types ◽  
Phase Iii ◽  
Definitive Treatment ◽  
Stroke Treatment ◽  
Portal Site ◽  
Therapy Studies ◽  
Phase Iii Studies

Abstract Definitive treatment of stroke constitutes an important thesis of regenerative medicine in the cerebrovascular field. However, to date, no cell therapy products for stroke are yet on the market. In this study, we examined the clinical research trends related to cell therapy products in the stroke field based on data obtained from the ClinicalTrials.gov website and International Clinical Trials Research Platform (ICTRP) portal site. These data do not offer results of clinical trials comprehensively but provide information regarding various attributes of planned clinical trials including work in progress. We selected 78 cell therapy studies related to the field of stroke treatment from ClinicalTrial.gov and ICTRP. These were analyzed according to, e.g., the reporting countries, origin (autologous or allogeneic), of cell used, cell types and source organs, the progress of translational phases, target phase of the disease (acute or chronic stroke), and route of administration. This analysis revealed a trend whereby in the acute phase, mesenchymal stem cells were administered intravenously at a relatively higher dose, whereas in the chronic phase a small number of cells were administered intracranially. Only two randomized controlled Phase III studies with over 100 patients are registered, but none of them has been completed. Thus, cell therapy against stroke appears to constitute a premature area compared with cartilage repair as assessed in our previous report. In addition, tracking by means of the ID number of each trial via PubMed revealed that 44% of clinical studies in this field have corresponding published results, which was also discussed.

scholarly journals Towards the standardization of stem cell therapy studies for ischemic heart diseases: Bridging the gap between animal models and the clinical setting

2017 ◽  
Vol 228 ◽  
pp. 465-480 ◽  
Author(s):  
Fábio Trindade ◽  
Adelino Leite-Moreira ◽  
João Ferreira-Martins ◽  
Rita Ferreira ◽  
Inês Falcão-Pires ◽  
...  
Keyword(s):  
Stem Cell ◽  
Animal Models ◽  
Cell Therapy ◽  
Stem Cell Therapy ◽  
Clinical Setting ◽  
Heart Diseases ◽  
Ischemic Heart ◽  
Ischemic Heart Diseases ◽  
Therapy Studies

Suitability of small diagnostic peripheral-blood samples for cell-therapy studies

Cytotherapy ◽  
2017 ◽  
Vol 19 (2) ◽  
pp. 311-326 ◽  
Author(s):  
Coralea Stephanou ◽  
Panayiota Papasavva ◽  
Myria Zachariou ◽  
Petros Patsali ◽  
Marilena Epitropou ◽  
...  
Keyword(s):  
Cell Therapy ◽  
Peripheral Blood ◽  
Blood Samples ◽  
Therapy Studies

scholarly journals Combined meta-analysis of preclinical cell therapy studies shows overlapping effect modifiers for multiple diseases

2021 ◽  
Vol 5 (1) ◽  
pp. e100061
Author(s):  
Peter-Paul Zwetsloot ◽  
Ana Antonic-Baker ◽  
Hendrik Gremmels ◽  
Kimberley Wever ◽  
Chris Sena ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Cell Therapy ◽  
Cell Type ◽  
Stage Of Development ◽  
Cord Injury ◽  
Multiple Variables ◽  
Meta Analyses ◽  
Therapy Studies ◽  
Research Domains

IntroductionCell therapy has been studied in many different research domains. Cellular replacement of damaged solid tissues is at an early stage of development, with much still to be understood. Systematic reviews and meta-analyses are widely used to aggregate data and find important patterns of results within research domains.We set out to find common biological denominators affecting efficacy in preclinical cell therapy studies for renal, neurological and cardiac disease.MethodsWe used datasets of five previously published meta-analyses investigating cell therapy in preclinical models of chronic kidney disease, spinal cord injury, stroke and ischaemic heart disease. We transformed primary outcomes to ratios of means to permit direct comparison across disease areas. Prespecified variables of interest were species, immunosuppression, cell type, cell origin, dose, delivery and timing of the cell therapy.ResultsThe five datasets from 506 publications yielded data from 13 638 animals. Animal size affects therapeutic efficacy in an inverse manner. Cell type influenced efficacy in multiple datasets differently, with no clear trend for specific cell types being superior. Immunosuppression showed a negative effect in spinal cord injury and a positive effect in cardiac ischaemic models. There was a dose–dependent relationship across the different models. Pretreatment seems to be superior compared with administration after the onset of disease.ConclusionsPreclinical cell therapy studies are affected by multiple variables, including species, immunosuppression, dose and treatment timing. These data are important when designing preclinical studies before commencing clinical trials.

scholarly journals Trophoblast stem cell marker gene expression in inner cell mass-derived cells from parthenogenetic equine embryos

Reproduction ◽  
2011 ◽  
Vol 141 (3) ◽  
pp. 321-332 ◽  
Author(s):  
Joëlle A Desmarais ◽  
Simon-Pierre Demers ◽  
Joao Suzuki ◽  
Simon Laflamme ◽  
Patrick Vincent ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Therapy ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Stem Cell Marker ◽  
Large Animal ◽  
Stem Cell Markers ◽  
Trophoblast Stem ◽  
Trophoblast Stem Cell ◽  
Therapy Studies

Although putative horse embryonic stem (ES)-like cell lines have been obtained recently from in vivo-derived embryos, it is currently not known whether it is possible to obtain ES cell (ESC) lines from somatic cell nuclear transfer (SCNT) and parthenogenetic (PA) embryos. Our aim is to establish culture conditions for the derivation of autologous ESC lines for cell therapy studies in an equine model. Our results indicate that both the use of early-stage blastocysts with a clearly visible inner cell mass (ICM) and the use of pronase to dissect the ICM allow the derivation of a higher proportion of primary ICM outgrowths from PA and SCNT embryos. Primary ICM outgrowths express the molecular markers of pluripotency POU class 5 homeobox 1 (POU5F1) and (sex determining region-Y)-box2 (SOX2), and in some cases, NANOG. Cells obtained after the passages of PA primary ICM outgrowths display alkaline phosphatase (AP) activity and POU5F1, SOX2, caudal-related homeobox-2 (CDX2) and eomesodermin (EOMES) expression, but may lose NANOG. Cystic embryoid body-like structures expressing POU5F1, CDX2 and EOMES were produced from these cells. Immunohistochemical analysis of equine embryos reveals the presence of POU5F1 in trophectoderm, primitive endoderm and ICM. These results suggest that cells obtained after passages of primary ICM outgrowths are positive for trophoblast stem cell markers while expressing POU5F1 and displaying AP activity. Therefore, these cells most likely represent trophoblast cells rather than true ESCs. This study represents an important first step towards the production of autologous equine ESCs for pre-clinical cell therapy studies on large animal models.

scholarly journals Isolation and characterization of dendritic cells from common marmosets for preclinical cell therapy studies

Immunology ◽  
2008 ◽  
Vol 123 (4) ◽  
pp. 566-574 ◽  
Author(s):  
Shigeki Ohta ◽  
Yoko Ueda ◽  
Masae Yaguchi ◽  
Yumi Matsuzaki ◽  
Masaya Nakamura ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Cell Therapy ◽  
Common Marmosets ◽  
Isolation And Characterization ◽  
Therapy Studies

An in vivo mouse model of human skin replacement for wound healing and cell therapy studies

2012 ◽  
Vol 65 (8) ◽  
pp. 1129-1131 ◽  
Author(s):  
Aihua Guo ◽  
W. Andrew Owens ◽  
Martin Coady ◽  
Dalie Liu ◽  
Colin A.B. Jahoda
Keyword(s):  
Wound Healing ◽  
Mouse Model ◽  
Cell Therapy ◽  
Human Skin ◽  
Skin Replacement ◽  
Therapy Studies

scholarly journals Corrigendum to “Modeling chronic cervical spinal cord injury in aged rats for cell therapy studies” [J. Clin. Neurosci., 94 (2021) 76–85/ISSN 0967-5868]

2021 ◽  
Author(s):  
María Martín-López ◽  
Elena González-Muñoz ◽  
Emilio Gómez-González ◽  
Rosario Sánchez-Pernaute ◽  
Javier Márquez-Rivas ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Cell Therapy ◽  
Cervical Spinal Cord ◽  
Cervical Spinal Cord Injury ◽  
Aged Rats ◽  
Cord Injury ◽  
Therapy Studies
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