scholarly journals Harnessing protein kinase A activation to induce mesenchymal-epithelial programs to eliminate chemoresistant, tumor-initiating breast cancer cells

2016 ◽  
Vol 5 (S2) ◽  
pp. S226-S232 ◽  
Author(s):  
Alex J. Gooding ◽  
William P. Schiemann
Keyword(s):  
Breast Cancer ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Kinase A

Leptin potentiates antiproliferative action of cAMP elevation via protein kinase A down-regulation in breast cancer cells

2010 ◽  
Vol 225 (3) ◽  
pp. 801-809 ◽  
Author(s):  
Silvio Naviglio ◽  
Davide Di Gesto ◽  
Fausto Illiano ◽  
Emilio Chiosi ◽  
Antonio Giordano ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Down Regulation ◽  
Antiproliferative Action ◽  
Camp Elevation ◽  
Kinase A

Forskolin improves sensitivity to doxorubicin of triple negative breast cancer cells via Protein Kinase A-mediated ERK1/2 inhibition

2018 ◽  
Vol 152 ◽  
pp. 104-113 ◽  
Author(s):  
Michela Illiano ◽  
Luigi Sapio ◽  
Alessia Salzillo ◽  
Lucia Capasso ◽  
Ilaria Caiafa ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Cancer Cells ◽  
Triple Negative Breast Cancer ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Kinase A

scholarly journals Delivery of Protein Kinase A by CRISPRMAX and Its Effects on Breast Cancer Stem-Like Properties

Pharmaceutics ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 11
Author(s):  
Jun-Nian Zhou ◽  
Tzu-Chen Rautio ◽  
Chang Liu ◽  
Xiao-Yu Xu ◽  
Dong-Qing Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Epithelial Mesenchymal Transition ◽  
Biological Effects ◽  
Gene Manipulation ◽  
Mesenchymal Transition ◽  
Kinase A

Protein kinase A (PKA) activation has recently been reported to inhibit epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) ability, which is considered to be responsible for chemoresistance and tumor recurrence in patients. While current studies mainly focus on gene manipulation of the EMT process, the direct delivery of PKA enzymes to cancer cells has never been investigated. Here, we utilize the commercial Lipofectamine CRISPRMAX reagent to directly deliver PKAs to breast cancer cells and evaluate its effects on EMT regulation. We optimized the delivery parameters with fluorescent-labeled bovine serum albumin, and successfully delivered fluorescent PKAs through CRISPRMAX into breast cancer cells. Then, we evaluated the biological effects by immunofluorescence, flow cytometry, mammosphere assay, and chemoresistance assay. Our data showed the expression of EMT-related markers, α-smooth muscle actin and N-cadherin, was downregulated after CRISPRMAX-PKA treatment. Although the CD44+/CD24− population did not change considerably, the size of mammospheres significantly decreased. In paclitaxel and doxorubicin chemoresistance assays, we noticed PKA delivery significantly inhibited paclitaxel resistance rather than doxorubicin resistance. Taken together, these results suggest our direct enzyme delivery can be a potential strategy for inhibiting EMT/CSC-associated traits, providing a safer approach and having more clinical translational efficacy than gene manipulation. This strategy will also facilitate the direct testing of other target enzymes/proteins on their biological functions.

Sign in / Sign up

Export Citation Format

Share Document