scholarly journals An ex vivo human placental vessel perfusion method to study mesenchymal stem/stromal cell migration

2019 ◽  
Vol 6 ◽  
pp. 2-2
Author(s):  
Balta Al-Sowayan ◽  
Rosemary J. Keogh ◽  
Mohammed Abumaree ◽  
Harry M. Georgiou ◽  
Bill Kalionis
Keyword(s):  
Cell Migration ◽  
Stromal Cell ◽  
Ex Vivo ◽  
Perfusion Method ◽  
Placental Vessel

scholarly journals New ex vivo model of corneal endothelial phacoemulsification injury and rescue therapy with mesenchymal stromal cell secretome

2019 ◽  
Vol 45 (3) ◽  
pp. 361-366 ◽  
Author(s):  
Majid Rouhbakhshzaeri ◽  
Behnam Rabiee ◽  
Nathalie Azar ◽  
Elham Ghahari ◽  
Ilham Putra ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Ex Vivo ◽  
Rescue Therapy ◽  
Ex Vivo Model

Attenuation of retinal endothelial cell migration and capillary morphogenesis in the absence of bcl-2

2008 ◽  
Vol 294 (6) ◽  
pp. C1521-C1530 ◽  
Author(s):  
Shuji Kondo ◽  
Yixin Tang ◽  
Elizabeth A. Scheef ◽  
Nader Sheibani ◽  
Christine M. Sorenson
Keyword(s):  
Cell Migration ◽  
Vascular System ◽  
Ex Vivo ◽  
Vascular Development ◽  
Critical Role ◽  
Endothelial Cell Migration ◽  
Cellular Functions ◽  
Capillary Morphogenesis ◽  
Angiogenesis Assay ◽  
Ischemic Retinopathy

Apoptosis plays a critical role during development and in the maintenance of the vascular system. B-cell leukemia lymphoma 2 (bcl-2) protects endothelial cells (EC) from apoptosis in response to a variety of stimuli. Previous work from this laboratory demonstrated attenuation of postnatal retinal vascular development and retinal neovascularization during oxygen-induced ischemic retinopathy in bcl-2-deficient (bcl-2−/−) mice. To gain further insight into the function of bcl-2 in the endothelium, we isolated retinal EC from bcl-2+/+ and bcl-2−/− mice. Retinal EC lacking bcl-2 demonstrated reduced cell migration, tenascin-C expression, and adhesion to vitronectin and fibronectin. The bcl-2−/− retinal EC also failed to undergo capillary morphogenesis in Matrigel. In addition, using an ex vivo angiogenesis assay, we observed reduced sprouting from aortic rings grown in culture from bcl-2−/− mice compared with bcl-2+/+ mice. Furthermore, reexpression of bcl-2 was sufficient to restore migration and capillary morphogenesis defects observed in bcl-2−/− retinal EC. Mechanistically, bcl-2−/− cells expressed significantly less endothelial nitric oxide synthase, an important downstream effecter of proangiogenic signaling. This may be attributed to increased oxidative stress in the absence of bcl-2. In fact, incubation of retinal EC or aortic rings from bcl-2−/− mice with the antioxidant N-acetylcysteine rescued their capillary morphogenesis and sprouting defects. Thus, bcl-2-mediated cellular functions play important roles not only in survival but also in proangiogenic phenotype of EC with a significant impact on vascular development and angiogenesis.

Stromal Cell-Derived Factor 1 Promotes Cell Migration to Enhance Bone Regeneration After Hypoxic Preconditioning

2019 ◽  
Vol 25 (17-18) ◽  
pp. 1300-1309 ◽  
Author(s):  
Jiaqi Huang ◽  
Hui Chi ◽  
Haiyang Chi ◽  
Lirong Qiu ◽  
Yufu Wang ◽  
...  
Keyword(s):  
Cell Migration ◽  
Bone Regeneration ◽  
Stromal Cell ◽  
Hypoxic Preconditioning ◽  
Stromal Cell Derived Factor

scholarly journals BONE MARROW-DERIVED MESENCHYMAL STEM CELLS UPREGULATE ENDOMETRIAL STROMAL CELL MIGRATION BUT NOT PROLIFERATION

2020 ◽  
Vol 114 (3) ◽  
pp. e100
Author(s):  
Qingshi Zhao ◽  
Yahaira Naaldijk ◽  
Oleta A. Sandiford ◽  
Nicole M. Marchetto ◽  
Nataki C. Douglas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Migration ◽  
Stromal Cell ◽  
Endometrial Stromal Cell
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