scholarly journals Human induced pluripotent stem cells illuminate pathways and novel treatment targets for age-related macular degeneration

2017 ◽  
pp. 92-92 ◽  
Author(s):  
Lindsay A. Farrer ◽  
Margaret M. DeAngelis
Keyword(s):  
Stem Cells ◽  
Macular Degeneration ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Age Related Macular Degeneration ◽  
Treatment Targets ◽  
Novel Treatment ◽  
Age Related ◽  
Induced Pluripotent

scholarly journals Small-molecule–directed, efficient generation of retinal pigment epithelium from human pluripotent stem cells

2015 ◽  
Vol 112 (35) ◽  
pp. 10950-10955 ◽  
Author(s):  
Julien Maruotti ◽  
Srinivas R. Sripathi ◽  
Kapil Bharti ◽  
John Fuller ◽  
Karl J. Wahlin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Small Molecule ◽  
Pluripotent Stem Cells ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Induced Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cells ◽  
Age Related Macular Degeneration ◽  
Age Related ◽  
Induced Pluripotent

Age-related macular degeneration (AMD) is associated with dysfunction and death of retinal pigment epithelial (RPE) cells. Cell-based approaches using RPE-like cells derived from human pluripotent stem cells (hPSCs) are being developed for AMD treatment. However, most efficient RPE differentiation protocols rely on complex, stepwise treatments and addition of growth factors, whereas small-molecule–only approaches developed to date display reduced yields. To identify new compounds that promote RPE differentiation, we developed and performed a high-throughput quantitative PCR screen complemented by a novel orthogonal human induced pluripotent stem cell (hiPSC)-based RPE reporter assay. Chetomin, an inhibitor of hypoxia-inducible factors, was found to strongly increase RPE differentiation; combination with nicotinamide resulted in conversion of over one-half of the differentiating cells into RPE. Single passage of the whole culture yielded a highly pure hPSC-RPE cell population that displayed many of the morphological, molecular, and functional characteristics of native RPE.

scholarly journals Generation of induced pluripotent stem cells derived from a 77-year-old healthy woman as control for age related diseases

2016 ◽  
Vol 17 (3) ◽  
pp. 550-552 ◽  
Author(s):  
Natakarn Nimsanor ◽  
Ida Jørring ◽  
Mikkel A. Rasmussen ◽  
Christian Clausen ◽  
Ulrike A. Mau-Holzmann ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Healthy Woman ◽  
Age Related ◽  
Induced Pluripotent

scholarly journals The Role of E3s in Regulating Pluripotency of Embryonic Stem Cells and Induced Pluripotent Stem Cells

2021 ◽  
Vol 22 (3) ◽  
pp. 1168
Author(s):  
Yahong Wu ◽  
Weiwei Zhang
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Regulatory Networks ◽  
Embryonic Stem ◽  
E3 Ligases ◽  
Somatic Cell Reprogramming ◽  
Age Related ◽  
Induced Pluripotent

Pluripotent embryonic stem cells (ESCs) are derived from early embryos and can differentiate into any type of cells in living organisms. Induced pluripotent stem cells (iPSCs) resemble ESCs, both of which serve as excellent sources to study early embryonic development and realize cell replacement therapies for age-related degenerative diseases and other cell dysfunction-related illnesses. To achieve these valuable applications, comprehensively understanding of the mechanisms underlying pluripotency maintenance and acquisition is critical. Ubiquitination modifies proteins with Ubiquitin (Ub) at the post-translational level to monitor protein stability and activity. It is extensively involved in pluripotency-specific regulatory networks in ESCs and iPSCs. Ubiquitination is achieved by sequential actions of the Ub-activating enzyme E1, Ub-conjugating enzyme E2, and Ub ligase E3. Compared with E1s and E2s, E3s are most abundant, responsible for substrate selectivity and functional diversity. In this review, we focus on E3 ligases to discuss recent progresses in understanding how they regulate pluripotency and somatic cell reprogramming through ubiquitinating core ESC regulators.

Sign in / Sign up

Export Citation Format

Share Document