scholarly journals Bone bridge formation across the neuroforamen 14 years after instrumented fusion for isthmic spondylolisthesis—a case report

2017 ◽  
Vol 3 (1) ◽  
pp. 76-81
Author(s):  
Joel Louis Lim ◽  
Kimberly-Anne Tan ◽  
Hwee Weng Dennis Hey
Keyword(s):  
Case Report ◽  
Isthmic Spondylolisthesis ◽  
Bone Bridge ◽  
Instrumented Fusion ◽  
Bridge Formation

scholarly journals Regeneration in Experimental Alveolar Bone Defect Using Human Umbilical Cord Mesenchymal Stem Cells

2021 ◽  
Vol 30 ◽  
pp. 096368972097539
Author(s):  
Akiko Toyota ◽  
Rei Shinagawa ◽  
Mikiko Mano ◽  
Kazuyuki Tokioka ◽  
Naoto Suda
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Cleft Lip ◽  
Human Umbilical Cord ◽  
Bone Surface ◽  
Bone Bridge ◽  
Bridge Formation ◽  
Alveolar Cleft ◽  
Umbilical Cords

Cleft lip and palate is a congenital disorder including cleft lip, and/or cleft palate, and/or alveolar cleft, with high incidence.The alveolar cleft causes morphological and functional abnormalities. To obtain bone bridge formation and continuous structure between alveolar clefts, surgical interventions are performed from infancy to childhood. However, desirable bone bridge formation is not obtained in many cases. Regenerative medicine using mesenchymal stem cells (MSCs) is expected to be a useful strategy to obtain sufficient bone bridge formation between alveolar clefts. In this study, we examined the effect of human umbilical cord-derived MSCs by transplantation into a rat experimental alveolar cleft model. Human umbilical cords were digested enzymatically and the isolated cells were collected (UC-EZ cells). Next, CD146-positive cells were enriched from UC-EZ cells by magnetic-activated cell sorting (UC-MACS cells). UC-EZ and UC-MACS cells showed MSC gene/protein expression, in vitro. Both cells had multipotency and could differentiate to osteogenic, chondrogenic, and adipogenic lineages under the differentiation-inducing media. However, UC-EZ cells lacked Sox2 expression and showed the lower ratio of MSCs than UC-MACS cells. Thus, UC-MACS cells were transplanted with hydroxyapatite and collagen (HA + Col) into alveolar cleft model to evaluate bone formation in vivo. The results of micro computed tomography and histological staining showed that UC-MACS cells with HA + Col induced more abundant bone formation between the experimental alveolar clefts than HA + Col implantation only. Cells immunopositive for osteopontin were accumulated along the bone surface and some of them were embedded in the bone. Cells immunopositive for human-specific mitochondria were aligned along the newly formed bone surface and in the new bone, suggesting that UC-MACS cells contributed to the bone bridge formation between alveolar clefts. These findings indicate that human umbilical cords are reliable bioresource and UC-MACS cells are useful for the alveolar cleft regeneration.

The spatial and temporal expression of VEGF and its receptors 1 and 2 in post-traumatic bone bridge formation of the growth plate

2011 ◽  
Vol 42 (6) ◽  
pp. 513-522 ◽  
Author(s):  
Eva Fischerauer ◽  
Nima Heidari ◽  
Bernhard Neumayer ◽  
Alexander Deutsch ◽  
Annelie M. Weinberg
Keyword(s):  
Growth Plate ◽  
Temporal Expression ◽  
Bone Bridge ◽  
Bridge Formation ◽  
Post Traumatic

scholarly journals Asymptomatic Cervical Isthmic Spondylolisthesis and Associated Occult Spinal Bifida: A Case Report

2013 ◽  
Vol 10 (1) ◽  
pp. 35 ◽  
Author(s):  
Jeong-Wook Lim ◽  
Sang-Kuk Kang ◽  
Su-Gi Jeon ◽  
Byeong-Chul Lim
Keyword(s):  
Case Report ◽  
Isthmic Spondylolisthesis

scholarly journals Application of Autologous Bone Marrow Derived Mesenchymal Stem Cells to an Ovine Model of Growth Plate Cartilage Injury

2010 ◽  
Vol 4 (1) ◽  
pp. 204-210 ◽  
Author(s):  
Rosa C McCarty ◽  
Cory J Xian ◽  
Stan Gronthos ◽  
Andrew C.W Zannettino ◽  
Bruce K Foster
Keyword(s):  
Bone Marrow ◽  
Animal Model ◽  
Growth Plate ◽  
Autologous Bone Marrow ◽  
Gelatin Sponge ◽  
Large Animal ◽  
Bone Bridge ◽  
Bridge Formation ◽  
Growth Plate Cartilage ◽  
Autologous Bone

Injury to growth plate cartilage in children can lead to bone bridge formation and result in bone growth deformities, a significant clinical problem currently lacking biological treatment. Mesenchymal stem/stromal cells (MSC) offer a promising therapeutic option for regeneration of damaged cartilage, due to their self renewing and multi-lineage differentiation attributes. Although some small animal model studies highlight the therapeutic potential of MSC for growth plate repair, translational research in large animal models, which more closely resemble the human condition, are lacking. Our laboratory has recently characterised MSCs derived from ovine bone marrow, and demonstrated these cells form cartilage-like tissue when transplanted within the gelatin sponge, Gelfoam,in vivo. In the current study, autologous bone marrow MSC were seeded into Gelfoam scaffold containing TGF-β1, and transplanted into a surgically created defect of the proximal ovine tibial growth plate. Examination of implants at 5 week post-operatively revealed transplanted autologous MSC failed to form new cartilage structure at the defect site, but contributed to an increase in formation of a dense fibrous tissue. Importantly, the extent of osteogenesis was diminished, and bone bridge formation was not accelerated due to transplantation of MSCs or the gelatin scaffold. The current study represents the first work that has utilised this ovine large animal model to investigate whether autologous bone marrow derived MSC can be used to initiate regeneration at the injured growth plate.

scholarly journals Adipose-derived stromal vascular fraction prevent bone bridge formation on growth plate injury in rat (in vivo studies) an experimental research

2020 ◽  
Vol 60 ◽  
pp. 211-217
Author(s):  
Panji Sananta ◽  
Rahaditya I Gede Made Oka ◽  
Prof Respati Suryanto Dradjat ◽  
Heri Suroto ◽  
Edi Mustamsir ◽  
...  
Keyword(s):  
Growth Plate ◽  
Experimental Research ◽  
Stromal Vascular Fraction ◽  
In Vivo Studies ◽  
Bone Bridge ◽  
Bridge Formation ◽  
Growth Plate Injury

scholarly journals C.04 Comparison of clinical outcomes between posterior instrumented fusion with and without interbody fusion for isthmic spondylolisthesis

2019 ◽  
Vol 46 (s1) ◽  
pp. S12
Author(s):  
V Chan ◽  
A Nataraj
Keyword(s):  
Length Of Stay ◽  
Clinical Outcomes ◽  
Interbody Fusion ◽  
Research Network ◽  
Leg Pain ◽  
Isthmic Spondylolisthesis ◽  
Instrumented Fusion ◽  
Multivariable Regression ◽  
Secondary Outcomes ◽  
Posterior Instrumented Fusion

Background: The purpose of this study is to compare 1-year postoperative clinical outcomes between posterior instrumented fusion with (P/TLIF) and without (PLF) interbody fusion in patients with isthmic spondylolisthesis. Methods: This is a multi-centre retrospective study using the Canadian Spine Outcomes and Research Network. Adult patients who received surgical management for isthmic spondylolisthesis were included in this study. The primary outcome was change in Oswestry Disability Index at 1-year. Secondary outcomes were change in EQ-5D, SF-12 PCS, back pain, leg pain, estimated blood loss, length of surgery, length of stay, rate of transfusions and adverse events. Descriptive statistics, Student t-test, Chi-Squared test, and stepwise multivariable regression were used for analysis. Results: A total of 300 patients (252 P/TLIF, 48 PLF) were included in this study. The mean age was 50 years. The P/TLIF group had poorer baseline leg pain scores (t=2.02, p=0.01). There were no significant differences in primary and secondary outcomes between the two groups. Addition of interbody fusion was not a significant variable in the stepwise multivariable regression analysis. Conclusions: There were no significant differences in clinical outcomes at 1 year. Addition of interbody fusion was not associated with higher complication rates or length of stay.

scholarly journals Assessment of pharmacokinetics for microvessel proliferation by DCE-MRI for early detection of physeal bone bridge formation in an animal model

2017 ◽  
Vol 30 (5) ◽  
pp. 417-427
Author(s):  
Bernhard Neumayer ◽  
Eva Amerstorfer ◽  
Clemens Diwoky ◽  
Richard A. Lindtner ◽  
Elisabeth Wadl ◽  
...  
Keyword(s):  
Animal Model ◽  
Early Detection ◽  
Bone Bridge ◽  
Dce Mri ◽  
Bridge Formation

Discitis and isthmic spondylolisthesis: a case report

2008 ◽  
Vol 17 (1) ◽  
pp. 39-41 ◽  
Author(s):  
Yossi Smorgick ◽  
Yizhar Floman ◽  
Yoram Anekstein ◽  
Reuven Shitrit ◽  
Leonel Copeliovitch ◽  
...  
Keyword(s):  
Case Report ◽  
Isthmic Spondylolisthesis
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