Automatic Cell Cutting by High-Precision Microfluidic Control

2011 ◽  
Vol 23 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Akihiko Ichikawa ◽  
◽  
Tamio Tanikawa ◽  
Satoshi Akagi ◽  
Kohtaro Ohba ◽  
...  
Keyword(s):  
High Precision ◽  
Microfluidic Chip ◽  
Microfluidic Channel ◽  
Volume Measurement ◽  
Microchannel Flow ◽  
Syringe Pump ◽  
Animal Cells ◽  
Positioning Control ◽  
System A ◽  
Image Volume

We have developed automated cell cutting by highprecision microfluidic control using a high-response and high-precision syringe pump. A microfluidic chip containing 2 orthogonal microchannels was used for cutting animal cells softened by cytochalasin and aspirated and fixed in 1 microchannel, then a high-velocity microchannel flow was generated from another channel to cut the cell. To control microchannel flow precisely, we made a syringe pump with a minimum flow of 0.35 × 10-3µl/min and response time of 10 ms. The syringe pump was connected to the microfluidic chip by a thin, hard Teflon tube to reduce the pressure transmission delay between the syringe pump and microfluidic channel. A microbead control experiment depending on PI control using the syringe pump was conducted to check the microchannel flow delay. Bovine oocytes softened by cytochalasin were injected into the microfluidic chip and bisected by microscopic image volume measurement. This paper reports the automatic cell cutting strategy and system, a result of microbead positioning control, and a result of automatic cell cutting.

Study on the Manufacture of Microfluidic Chip with Coated Glass

2012 ◽  
Vol 548 ◽  
pp. 254-257 ◽  
Author(s):  
Yan He ◽  
Bai Ling Huang ◽  
Yong Lai Zhang ◽  
Li Gang Niu
Keyword(s):  
Microfluidic Chip ◽  
Low Cost ◽  
Microfluidic Channel ◽  
Critical Factor ◽  
Wet Etching ◽  
Microfluidic Chips ◽  
Etching Technique ◽  
Chip Quality ◽  
Wet Chemical ◽  
Wet Chemical Etching

In this paper, a simple and facile technique for manufacturing glass-based microfluidic chips was developed. Instead of using expensive dry etching technology, the standard UV lithography and wet chemical etching technique was used to fabricate microchannels on a K9 glass substrate. The fabrication process of microfluidic chip including vacuum evaporation, annealing, lithography, and BHF (HF-NH4F-H2O) wet etching were investigated. Through series experiments, we found that anneal was the critical factor for chip quality. As a representative example, a microfluidic channel with 20 m of depth, and 80 m of width was successfully prepared, and the channel surfaces are quite smooth. These results present a simple, low cost, flexible and easy way to fabricate glass-based microfluidic chips.

Anti-windup reconfigurable control for dynamic positioning vessel with thruster faults

2020 ◽  
Vol 42 (16) ◽  
pp. 3216-3224
Author(s):  
Mingyang Li ◽  
Wenbo Xie ◽  
Jian Zhang
Keyword(s):  
Control Method ◽  
Sliding Mode ◽  
Input Saturation ◽  
Control Process ◽  
Dynamic Positioning ◽  
Closed Loop System ◽  
Reconfigurable Control ◽  
Positioning Control ◽  
Dynamic Positioning System ◽  
System A

In this study, an anti-windup reconfigurable control method is developed for dynamic positioning vessel in the presence of thruster faults and input saturation. The designed reconfiguration block acting as a virtual thruster aims at hiding the faults from the nominal controller. Also, it is added into the closed-loop system between the nominal controller and the dynamic positioning system. A thruster saturation-failure fault matrix technique is proposed to regard the thruster saturation as thruster fault, meanwhile an auxiliary system is constructed to achieve extra compensation for the adverse effects induced by input saturation. Furthermore, an integral sliding mode control method is presented to accommodate the nonlinear items in the reconfiguration block. An adaptive technique is also employed to preserve robustness against the unknown uncertainties. Finally, a vessel dynamic positioning control process is adopted to evaluate the effectiveness of the proposed method.

High-Precision Wire-EDM by Online Wire Positioning Control

CIRP Annals ◽  
1994 ◽  
Vol 43 (1) ◽  
pp. 193-197 ◽  
Author(s):  
D.F. Dauw ◽  
I. Beltrami
Keyword(s):  
High Precision ◽  
Wire Edm ◽  
Positioning Control

Machine vision for high-precision volume measurement applied to levitated containerless material processing

2005 ◽  
Vol 76 (12) ◽  
pp. 125108 ◽  
Author(s):  
R. C. Bradshaw ◽  
D. P. Schmidt ◽  
J. R. Rogers ◽  
K. F. Kelton ◽  
R. W. Hyers
Keyword(s):  
Material Processing ◽  
Machine Vision ◽  
High Precision ◽  
Volume Measurement
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