scholarly journals Simultaneous Electrochemiluminescence Determination of Sinomenine, Cepharanthine and Tetrahydropalmatine in Stephania epigaea by Capillary Electrophoresis Coupled with Ultrasonic-Assisted Aqueous Two-Phase Extraction

2020 ◽  
pp. 5002-5017
Author(s):  
Shuangjiao Sun ◽  
Keyword(s):  
Capillary Electrophoresis ◽  
Phase Extraction ◽  
Two Phase ◽  
Ultrasonic Assisted ◽  
Aqueous Two Phase Extraction

scholarly journals A Novel and Sensitive Method for the Determination of VitaminB2(Riboflavin) in Urine and Pharmaceutical Samples Using an Aqueous Two-Phase Extraction

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Sabah Shiri ◽  
Tahere Khezeli ◽  
Sami Sajjadifar ◽  
Ali Delpisheh ◽  
Moayed Avazpour ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Standard Deviation ◽  
Limit Of Detection ◽  
Vitamin B ◽  
Phase Extraction ◽  
Two Phase ◽  
Pharmaceutical Samples ◽  
Relative Standard ◽  
Aqueous Two Phase Extraction

A novel, simple, and more sensitive spectrophotometric procedure has been developed for the determination of vitamin B2(riboflavin) by an aqueous two-phase extraction (ATPE). An ATPE is formed mostly by water and does not require an organic solvent. Other ATPE components used in this study were the polymer, polyethylene glycol (PEG), and some salts such as Na2SO4and Na2CO3. The method is based on the interaction between vitamin B2(riboflavin) and sodium sulfate (Na2SO4) in an acidic medium (pH 6.4). The influences of effective parameters such as salt (type and concentration), polyethylene glycol (molecular weight and concentration), temperature, centrifuging time, and pH of the sample solution were studied and optimized. The linear range was 1.3–320 ng/mL (R2=0.9991;n=10) with the relative standard deviation (RSD) for 60 ng/mL 3.68%. The limit of detection (LOD) calculated from three times of standard deviation of blank were 0.2 ng/mL and recoveries from analysis of real samples between 94.82% and 103.98% were obtained for the determination of vitamin B2(riboflavin) in urine and pharmaceutical samples.

Determination of common ginsenosides in Kang'ai injection by aqueous two-phase extraction with deep eutectic solvents and HPLC-UV/DAD

2018 ◽  
Vol 137 ◽  
pp. 302-308 ◽  
Author(s):  
Peng Li ◽  
Pengyun Zhao ◽  
Weijie Liu ◽  
Yifu Jiang ◽  
Wenjiao Wang ◽  
...  
Keyword(s):  
Deep Eutectic Solvents ◽  
Phase Extraction ◽  
Two Phase ◽  
Aqueous Two Phase Extraction

Determination of steroid hormones in milk using aqueous two-phase extraction coupled to liquid chromatography

2015 ◽  
Vol 7 (6) ◽  
pp. 2514-2522 ◽  
Author(s):  
Na Li ◽  
Xiao Yang ◽  
Li Nian ◽  
Zhibing Wang ◽  
Lei Lei ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Steroid Hormones ◽  
Phase System ◽  
Phase Extraction ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Aqueous Two Phase Extraction

An acetonitrile (ACN)-salt based aqueous two-phase system was developed and applied to the extraction of steroid hormones in milk.

scholarly journals Flavonoids from Fig (Ficus carica Linn.) Leaves: The Development of a New Extraction Method and Identification by UPLC-QTOF-MS/MS

2021 ◽  
Vol 11 (16) ◽  
pp. 7718
Author(s):  
Chunjian Zhao ◽  
Shen Li ◽  
Chunying Li ◽  
Tingting Wang ◽  
Yao Tian ◽  
...  
Keyword(s):  
Soxhlet Extraction ◽  
Ficus Carica ◽  
Total Flavonoids ◽  
Phase Extraction ◽  
Two Phase ◽  
Solid Ratio ◽  
Ultrasonic Time ◽  
Ultrasonic Assisted ◽  
Potential Applications ◽  
Aqueous Two Phase Extraction

Flavonoid-rich leaves of the Ficus carica L. plant are usually discarded as waste. In this work, ultrasonic enzyme-assisted aqueous two-phase extraction (UEAATPE) was proposed as an innovative method to estimate the total flavonoids present in F. carica L. leaves. Total flavonoids were analyzed qualitatively and quantitatively by UPLC-QTOF-MS. At 38% (w/w) ethanol/18% (w/w) ammonium sulfate, we achieved the optimum conditions in which to establish an easy-to-form aqueous two-phase extraction (ATPE) as the final system. The optimal UEAATPE conditions were set at an enzymatic concentration of 0.4 U/g, 150 min enzymolysis time, an enzymolysis temperature of 50 °C, a liquid–solid ratio of 20:1 (mL/g), and 30 min ultrasonic time. The yields of the total flavonoids, i.e., 60.22 mg/g, obtained by UEAATPE were found to be 1.13-fold, 1.21-fold, 1.27-fold, and 2.43-fold higher than those obtained by enzyme-assisted ATPE (EAATPE), ultrasonic-assisted ATPE (UAATPE), ATPE, and soxhlet extraction (SE) methods, respectively. Furthermore, eleven flavonoids from the leaves of the F. carica L. plant were completely identified and fully characterized. Among them, ten flavonoids have been identified for the first time from the leaves of the F. carica L. plant. These flavonoids are quercetin 3-O-hexobioside-7-O-hexoside, 2-carboxyl-1,4-naphthohydroquinone-4-O-hexoside, luteolin 6-C-hexoside, 8-C-pentoside, kaempferol 6-C-hexoside-8-C-hexoside, quercetin 6-C-hexobioside, kaempferol 6-C-hexoside-8-C-hexoside, apigenin 2″-O-pentoside, apigenin 6-C-hexoside, quercetin 3-O-hexoside, and kaempferol 3-O-hexobioside. Therefore, F. carica L. leaves contain new kinds of unidentified natural flavonoids and are a rich source of biological activity. Therefore, this research has potential applications and great value in waste handling and utilization.

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