scholarly journals A Nonenzymatic Sensor Based on {PEI/[P2W17V-CNTs/PEI- CuO]10/P2W17V-CNTs} Nanocomposite Film for the Sensitive Determination of Iodate

2018 ◽  
pp. 8454-8470
Author(s):  
Xinming Wang ◽  
Keyword(s):  
Nanocomposite Film ◽  
Nonenzymatic Sensor ◽  
Sensitive Determination

Development of a colorimetric protein phosphorylation assay for detecting cyanobacterial toxins

1995 ◽  
Vol 31 (5-6) ◽  
pp. 47-49 ◽  
Author(s):  
C. Ash ◽  
C. MacKintosh ◽  
R. MacKintosh ◽  
C. R. Fricker
Keyword(s):  
Protein Phosphorylation ◽  
Colorimetric Assay ◽  
Cyanobacterial Toxins ◽  
Sensitive Determination

A new colorimetric assay is described, based on inhibition of protein phosphotases, that enables the rapid, simple and sensitive determination of the concentration of toxins from cyanobacteria.

Non-enzymatic determination of purine nucleotides using a carbon dot modified glassy carbon electrode

2019 ◽  
Vol 11 (30) ◽  
pp. 3866-3873 ◽  
Author(s):  
R. Karthikeyan ◽  
D. James Nelson ◽  
S. Abraham John
Keyword(s):  
Glassy Carbon ◽  
Low Cost ◽  
Phosphate Buffer Solution ◽  
Purine Nucleotides ◽  
Buffer Solution ◽  
Solution Ph ◽  
Carbon Dot ◽  
Enzymatic Determination ◽  
Sensitive Determination

Selective and sensitive determination of one of the purine nucleotides, inosine (INO) using a low cost carbon dot (CD) modified glassy carbon (GC) electrode in 0.2 M phosphate buffer solution (pH 7.2) was demonstrated in this paper.

Cross Double Point Discharge as Enhanced Excitation Source for Highly Sensitive Determination of Arsenic, Mercury and Lead by Optical Emission Spectrometry

2021 ◽  
Author(s):  
Lin He ◽  
Peixia Li ◽  
Kai Li ◽  
Tao Lin ◽  
Jin Luo ◽  
...  
Keyword(s):  
Double Point ◽  
Hydride Generation ◽  
Optical Emission ◽  
Sample Introduction ◽  
Excitation Source ◽  
Emission Spectrometry ◽  
Highly Sensitive ◽  
Point Discharge ◽  
Sensitive Determination

A new cross double point discharge (CrossPD) microplasma was designed as an excitation source to construct a miniaturized optical emission spectrometer with hydride generation (HG) for sample introduction. The CrossPD...

scholarly journals Fast and Sensitive Determination of the Fungicide Carbendazim in Fruit Juices with an Immunosensor Based on White Light Reflectance Spectroscopy

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 153
Author(s):  
Georgios Koukouvinos ◽  
Chrysoula-Evangelia Karachaliou ◽  
Ioannis Raptis ◽  
Panagiota Petrou ◽  
Evangelia Livaniou ◽  
...  
Keyword(s):  
White Light ◽  
Optical Biosensor ◽  
Reflectance Spectroscopy ◽  
Fruit Juices ◽  
Sample Treatment ◽  
Assay Sensitivity ◽  
Reliable Determination ◽  
Light Reflectance ◽  
Sensitive Determination

Carbendazim is a systemic benzimidazole-type fungicide with broad-spectrum activity against fungi that undermine food products safety and quality. Despite its effectiveness, carbendazim constitutes a major environmental pollutant, being hazardous to both humans and animals. Therefore, fast and reliable determination of carbendazim levels in water, soil, and food samples is of high importance for both food industry and public health. Herein, an optical biosensor based on white light reflectance spectroscopy (WLRS) for fast and sensitive determination of carbendazim in fruit juices is presented. The transducer is a Si/SiO2 chip functionalized with a benzimidazole conjugate, and determination is based on a competitive immunoassay format. Thus, for the assay, a mixture of an in-house developed rabbit polyclonal anti-carbendazim antibody with the standards or samples is pumped over the chip, followed by biotinylated secondary antibody and streptavidin. The WLRS platform allows for real-time monitoring of biomolecular interactions carried out onto the Si/SiO2 chip by transforming the shift in the reflected interference spectrum caused by the immunoreaction to effective biomolecular adlayer thickness. The sensor is able to detect 20 ng/mL of carbendazim in fruit juices with high accuracy and precision (intra- and inter-assay CVs ≤ 6.9% and ≤9.4%, respectively) in less than 30 min, applying a simple sample treatment that alleviates any “matrix-effect” on the assay results and a 60 min preincubation step for improving assay sensitivity. Excellent analytical characteristics and short analysis time along with its small size render the proposed WLRS immunosensor ideal for future on-the-spot determination of carbendazim in food and environmental samples.

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