scholarly journals Fabrication of an Electrochemical Biosensor Based on Nafion/Horseradish Peroxidase/Co3O4 NP/CILE and Its Electrocatalysis

Author(s):  
Wei Chen ◽  
2011 ◽  
Vol 239-242 ◽  
pp. 2499-2502
Author(s):  
Nan Nan Wei ◽  
Jiang Yan Du

A new electrochemical biosensor based on the immobilization of horseradish peroxidase(HRP) on TiO2 nanoneedles modified electrodes has been fabricated. The direct electrochemical response of HRP immobilized on the modified electrode was dramatically enhanced. The immobilized HRP displayed a couple of stable and well-defined redox peaks with a formal potential of -0.379 V (vs. SCE). The HRP/TiO2 nanoneedles modified electrode exhibited a remarkable electrocatalytic activity toward the oxidation of H2O2. The amperometric response to H2O2 showed a linear range of 4–700μmol/L, with the calculated detection limit of 0.78 μmol/L at a signal-to-noise ratio of three. The modified electrode displayed an acceptable reproducibility and good stability. The new HRP/TiO2 nanoneedles matrix is expected to have wide applications for enzymes and proteins immobilization and direct electron transfer study and opened a way for low conductivity electrode biosensors.


RSC Advances ◽  
2020 ◽  
Vol 10 (15) ◽  
pp. 9079-9087 ◽  
Author(s):  
Kamila Spychalska ◽  
Dorota Zając ◽  
Joanna Cabaj

A convenient electrochemical sensing pathway for 17β-estradiol detection was investigated.


2003 ◽  
Vol 18 (5-6) ◽  
pp. 715-720 ◽  
Author(s):  
Aoife Morrin ◽  
Alicia Guzman ◽  
Anthony J. Killard ◽  
Jose M. Pingarron ◽  
Malcolm R. Smyth

2007 ◽  
Vol 30 (4) ◽  
pp. 289-296 ◽  
Author(s):  
Jianwen Wang ◽  
Ming Gu ◽  
Junwei Di ◽  
Yansheng Gao ◽  
Ying Wu ◽  
...  

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