scholarly journals One Step Electrodeposition of Graphene-Au Nanocomposites for Highly Sensitive Electrochemical Detection of Salbutamol

Author(s):  
Xiu-Chun Guo ◽  
2015 ◽  
Vol 7 (21) ◽  
pp. 9261-9267 ◽  
Author(s):  
Rui Wang ◽  
Kangbing Wu ◽  
Can Wu

Graphene sheets (GSs) are first easily prepared via one-step solvent exfoliation of graphite powder in N-methyl-2-pyrrolidone (NMP) and then hybridized with Ni(OH)2 microparticles.


2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Yang Zhang ◽  
Chunyang Dai ◽  
Huiyan Wang ◽  
Yong Gao ◽  
Tuantuan Li ◽  
...  

Abstract Background Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, is posing a serious threat to global public health. Reverse transcriptase real-time quantitative polymerase chain reaction (qRT-PCR) is widely used as the gold standard for clinical detection of SARS-CoV-2. Due to technical limitations, the reported positive rates of qRT-PCR assay of throat swab samples vary from 30 to 60%. Therefore, the evaluation of alternative strategies to overcome the limitations of qRT-PCR is required. A previous study reported that one-step nested (OSN)-qRT-PCR revealed better suitability for detecting SARS-CoV-2. However, information on the analytical performance of OSN-qRT-PCR is insufficient. Method In this study, we aimed to analyze OSN-qRT-PCR by comparing it with droplet digital PCR (ddPCR) and qRT-PCR by using a dilution series of SARS-CoV-2 pseudoviral RNA and a quality assessment panel. The clinical performance of OSN-qRT-PCR was also validated and compared with ddPCR and qRT-PCR using specimens from COVID-19 patients. Result The limit of detection (copies/ml) of qRT-PCR, ddPCR, and OSN-qRT-PCR were 520.1 (95% CI: 363.23–1145.69) for ORF1ab and 528.1 (95% CI: 347.7–1248.7) for N, 401.8 (95% CI: 284.8–938.3) for ORF1ab and 336.8 (95% CI: 244.6–792.5) for N, and 194.74 (95% CI: 139.7–430.9) for ORF1ab and 189.1 (95% CI: 130.9–433.9) for N, respectively. Of the 34 clinical samples from COVID-19 patients, the positive rates of OSN-qRT-PCR, ddPCR, and qRT-PCR were 82.35% (28/34), 67.65% (23/34), and 58.82% (20/34), respectively. Conclusion In conclusion, the highly sensitive and specific OSN-qRT-PCR assay is superior to ddPCR and qRT-PCR assays, showing great potential as a technique for detection of SARS-CoV-2 in patients with low viral loads.


2021 ◽  
Vol 26 ◽  
pp. 102005
Author(s):  
Balla Fall ◽  
Abdou K.D. Diaw ◽  
Modou Fall ◽  
Mohamed L. Sall ◽  
Momath Lo ◽  
...  

2011 ◽  
Vol 8 ◽  
pp. 248-256 ◽  
Author(s):  
Hong-wei Yu ◽  
Yuan-sheng Wang ◽  
Yu li ◽  
Guo-Li Shen ◽  
Hai-long Wu ◽  
...  

2021 ◽  
pp. 106770
Author(s):  
Lei Fan ◽  
Yuying Xin ◽  
Yingming Xu ◽  
Xianfa Zhang ◽  
Xiaoli Cheng ◽  
...  

Author(s):  
Navaneeth Punnakkal ◽  
Jeethu Raveendran ◽  
Suneesh Punathil Vasu ◽  
Bipin G Nair ◽  
T G Satheesh Babu

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