scholarly journals A Glucose Biosensor Based on Glucose Oxidase enzyme and ZnO Nanoparticles Modified Carbon Paste Electrode

2016 ◽  
pp. 9891-9901 ◽  
Author(s):  
Ali Shamsazar ◽  
Keyword(s):  
Glucose Oxidase ◽  
Carbon Paste Electrode ◽  
Zno Nanoparticles ◽  
Glucose Biosensor ◽  
Modified Carbon Paste Electrode ◽  
Carbon Paste ◽  
Oxidase Enzyme ◽  
Paste Electrode

Synthesis of Fructose Biosensors and Progressing Their Efficiency Using Californium Colloidal Nanoparticles for Detecting Fructose and Triglycerides

2020 ◽  
Vol 12 (8) ◽  
pp. 1002-1017
Author(s):  
Alireza Heidari
Keyword(s):  
Carbon Paste Electrode ◽  
Enzyme Reaction ◽  
The Other ◽  
Current Paper ◽  
Modified Carbon Paste Electrode ◽  
Colloidal Nanoparticles ◽  
Carbon Paste ◽  
High Concentration ◽  
Oxidase Enzyme ◽  
Paste Electrode

In the current paper, fructose–oxidase enzyme is used as stabilization medium due to its more efficiency, ability for more accurate controlling the enzyme reaction, protecting against wasting of enzyme as well as simple and easy use and exchange of enzyme medium after performing some levels of surface modification and developing multi–walled carbon nanotubes (MWCNTs) on Californium plate. For better connecting and stabilizing the enzyme on the medium, the prepared medium is washed by high concentration sulfuric acid and nitric acid and a large volume of deionized water and for protecting enzyme from devastating effect of Californium and prohibiting them to become inactive, surface is covered with cystamine before stabilization. Regarding the large size of fructose–oxidase enzyme compared to surface of medium, a connective material with amid at one end and pyrine at the other end is used as transfer agent and for stabilizing this connection, the prepared medium is placed into dimethylformamide (DMF) solution for a couple of hours. Activity of stabilized enzyme at 460 (nm) wavelength recorded by spectroscope was depicted against time to evaluate its stability in various times. The prepared medium, which have a large amount of fructose–oxidase enzyme, can be used as electrode in sensors. Furthermore, fructose–oxidase electrochemical sensor is one of the best methods for detecting low amount of fructose and applying Californium colloidal nanoparticles as a supplementary material in the structure of biosensor can be effective for progressing its efficiency and optimum efficiency. On the other hand, in the current study, electrode biosensor entitled as modified carbon paste electrode with Californium colloidal nanoparticles (Cfnano/CPE) is produced by carbon graphite powder, paraffin oil and Californium colloidal nanoparticles (24 nm) and it is compared with carbon paste electrode (CP). In semi–permeable membranes, a combination of 1 (ml) of 0.1 (M) phosphate buffer with specified pH and 10 (mg) of fructose–oxidase enzyme is placed around each electrode. In the same potential of 0.7 (V), biosensors are tested with fructose in concentration range of (0−1) (mM) and various amounts of pH (4,6,8) which lead to producing the maximum current and tracing fructose in pH=6 and concentration of 1 (M) as the optimum condition. Currentmetry induced from both biosensors are compared and it is confirmed that using Californium colloidal nanoparticles in the structure of (Cfnano/CPE) electrode leads to increasing the conductivity and currentmerty of biosensor. In addition, qualitative and quantitative measurement of food components is of great importance due to high cost of traditional methods, in addition to tendency for more accurate and sensitive detecting of these components. Fructose and triglycerides are such compounds that they frequently measure. Various methods are used to detect these food elements. However, the necessity for accurate measurement of these two compounds with high sensitivity, especially for food health issue, leads to developing biological methods, especially biosensors. Among them, biosensors based on conductive polymer nanostructures, especially Polypyrrole and Polythiophene, have been recently interested due to their unique characteristics. The current paper aims to introduce and investigate the previously performed studies about Polypyrrole and Polythiophene–based biosensors for detecting fructose and triglycerides.

Glucose biosensor based on immobilization of glucose oxidase on a carbon paste electrode modified with microsphere-attached l -glycine

2017 ◽  
Vol 64 (5) ◽  
pp. 745-753 ◽  
Author(s):  
Soner Donmez ◽  
Fatma Arslan ◽  
Nurşen Sarı ◽  
Elvan Hasanoğlu Özkan ◽  
Halit Arslan
Keyword(s):  
Glucose Oxidase ◽  
Carbon Paste Electrode ◽  
Glucose Biosensor ◽  
Carbon Paste ◽  
Paste Electrode

Electrochemical glucose biosensor based on nickel oxide nanoparticle-modified carbon paste electrode

2013 ◽  
Vol 42 (4) ◽  
pp. 237-244 ◽  
Author(s):  
Ceren Erdem ◽  
Derya Koyuncu Zeybek ◽  
Gözde Aydoğdu ◽  
Bülent Zeybek ◽  
Şule Pekyardımcı ◽  
...  
Keyword(s):  
Nickel Oxide ◽  
Carbon Paste Electrode ◽  
Glucose Biosensor ◽  
Modified Carbon Paste Electrode ◽  
Carbon Paste ◽  
Nickel Oxide Nanoparticle ◽  
Oxide Nanoparticle ◽  
Paste Electrode

scholarly journals Direct Electron Transfer of Cytochrome c on ZnO Nanoparticles Modified Carbon Paste Electrode

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Negahdary ◽  
Saeed Rezaei-Zarchi ◽  
Neda Rousta ◽  
Soheila Samei Pour
Keyword(s):  
Cytochrome C ◽  
Carbon Paste Electrode ◽  
Zno Nanoparticles ◽  
Direct Electron Transfer ◽  
Direct Electrochemistry ◽  
Phosphate Buffer Solution ◽  
Modified Carbon Paste Electrode ◽  
Carbon Paste ◽  
Buffer Solution ◽  
Paste Electrode

The direct electrochemistry of cytochrome c (cyt c) immobilized on a modified carbon paste electrode (CPE) was described. The electrode was modified with ZnO nanoparticles. Direct electrochemistry of cytochrome c in this paste electrode was easily achieved, and a pair of well-defined quasireversible redox peaks of a heme Fe (III)/Fe(II) couple appeared with a formal potential (E0) of −0.303 V (versus SCE) in pH 7.0 phosphate buffer solution (PBS). The fabricated modified bioelectrode showed good electrocatalytic ability for reduction of H2O2. The preparation process of the proposed biosensor was convenient, and the resulting biosensor showed high sensitivity, low detection limit, and good stability.

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