scholarly journals Comparison of gene expression profile between blood cells and white adipose tissue of patients with obesity

2017 ◽  
Vol 34 (3) ◽  
pp. 608 ◽  
Author(s):  
Marcela Augusta de Souza Pinhel ◽  
Natália Yumi Noronha ◽  
Carolina Ferreira Nicoletti ◽  
Driele Cristina Gomes Quinhoneiro ◽  
Bruno Affonso Parenti Oliveira ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
White Adipose Tissue ◽  
Blood Cells

scholarly journals Gene expression profile of mouse white adipose tissue during inflammatory stress: age-dependent upregulation of major procoagulant factors

Aging Cell ◽  
2013 ◽  
Vol 12 (2) ◽  
pp. 194-206 ◽  
Author(s):  
Marlene E. Starr ◽  
Yanling Hu ◽  
Arnold J. Stromberg ◽  
Joseph R. Carmical ◽  
Thomas G. Wood ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
White Adipose Tissue ◽  
Inflammatory Stress ◽  
Age Dependent

scholarly journals Differences in the Inflammatory Response of White Adipose Tissue and Adipose-Derived Stem Cells

2020 ◽  
Vol 21 (3) ◽  
pp. 1086
Author(s):  
Sara Taha ◽  
Elias Volkmer ◽  
Elisabeth Haas ◽  
Paolo Alberton ◽  
Tobias Straub ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Inflammatory Response ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
White Adipose Tissue ◽  
Inflammatory Responses ◽  
System Response ◽  
Adipose Derived Stem Cells

The application of liposuctioned white adipose tissue (L-WAT) and adipose-derived stem cells (ADSCs) as a novel immunomodulatory treatment option is the currently subject of various clinical trials. Because it is crucial to understand the underlying therapeutic mechanisms, the latest studies focused on the immunomodulatory functions of L-WAT or ADSCs. However, studies that examine the specific transcriptional adaptation of these treatment options to an extrinsic inflammatory stimulus in an unbiased manner are scarce. The aim of this study was to compare the gene expression profile of L-WAT and ADSCs, when subjected to tumor necrosis factor alpha (TNFα), and to identify key factors that might be therapeutically relevant when using L-WAT or ADSCs as an immuno-modulator. Fat tissue was harvested by liposuction from five human donors. ADSCs were isolated from the same donors and shortly subjected to expansion culture. L-WAT and ADSCs were treated with human recombinant TNFα, to trigger a strong inflammatory response. Subsequently, an mRNA deep next-generation sequencing was performed to evaluate the different inflammatory responses of L-WAT and ADSCs. We found significant gene expression changes in both experimental groups after TNFα incubation. However, ADSCs showed a more homogenous gene expression profile by predominantly expressing genes involved in immunomodulatory processes such as CCL19, CCL5, TNFSF15 and IL1b when compared to L-WAT, which reacted rather heterogeneously. As RNA sequencing between L-WAT and ADSCS treated with TNFα revealed that L-WAT responded very heterogeneously to TNFα treatment, we therefore conclude that ADSCs are more reliable and predictable when used therapeutically. Our study furthermore yields insight into potential biological processes regarding immune system response, inflammatory response, and cell activation. Our results can help to better understand the different immunomodulatory effects of L-WAT and ADSCs.

scholarly journals Gene expression profile induced by leptin in white adipose tissue and liver

10.1038/14405 ◽  
1999 ◽  
Vol 23 (S3) ◽  
pp. 75-75 ◽  
Author(s):  
Alexander Soukas ◽  
Paul Cohen ◽  
Jeffrey M. Friedman
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
White Adipose Tissue

scholarly journals Cultured Human Adipose Tissue Pericytes and Mesenchymal Stromal Cells Display a Very Similar Gene Expression Profile

2015 ◽  
Vol 24 (23) ◽  
pp. 2822-2840 ◽  
Author(s):  
Lindolfo da Silva Meirelles ◽  
Tathiane Maistro Malta ◽  
Virgínia Mara de Deus Wagatsuma ◽  
Patrícia Viana Bonini Palma ◽  
Amélia Goes Araújo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Gene Expression Profile ◽  
Mesenchymal Stromal Cells ◽  
Expression Profile ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
Similar Gene Expression Profile ◽  
Similar Gene

Altered complement gene expression profile of adipose tissue and adipocytes in obesity: A monozygotic twin study

Immunobiology ◽  
2016 ◽  
Vol 221 (10) ◽  
pp. 1187
Author(s):  
Sanna Kaye ◽  
Anna Hanttu ◽  
A. Inkeri Lokki ◽  
Eija Nissilä ◽  
Sini Heinonen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Twin Study ◽  
Monozygotic Twin ◽  
Complement Gene
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