scholarly journals Relationship between BsmI polymorphism and VDR gene methylation profile, gender, metabolic profile, oxidative stress, and inflammation in adolescents

2021 ◽  
Author(s):  
Maria Paula de Paiva ◽  
Lavoisiana Lacerda de Lucena ◽  
Alexandre Sérgio Silva ◽  
Rayner Anderson Ferreira do Nascimento ◽  
Darlene Camati Persuhn ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Metabolic Profile ◽  
Methylation Profile ◽  
Gene Methylation ◽  
Vdr Gene ◽  
Bsmi Polymorphism

Effect of vitamin D3 supplementation and influence of BsmI polymorphism of the VDR gene of the inflammatory profile and oxidative stress in elderly women with vitamin D insufficiency

2015 ◽  
Vol 66 ◽  
pp. 10-16 ◽  
Author(s):  
Isa Gabriela de Medeiros Cavalcante ◽  
Alexandre Sérgio Silva ◽  
Maria José Carvalho Costa ◽  
Darlene Camati Persuhn ◽  
ChariraTahaMad Ibraim Issa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin D ◽  
Vitamin D3 ◽  
Elderly Women ◽  
Vitamin D Insufficiency ◽  
Vdr Gene ◽  
Bsmi Polymorphism ◽  
Vitamin D3 Supplementation ◽  
Inflammatory Profile ◽  
And Oxidative Stress

Effect of Probiotic and Amla Powder Supplementation on Metabolic Profile and Milk Production of Summer Stressed Buffaloes Residing in Heavy Metal Polluted Areas of Ludhiana

2021 ◽  
Author(s):  
Pranesh V. Yeotikar ◽  
Shashi Nayyar ◽  
Chanchal Singh ◽  
C.S. Mukhopadhyay ◽  
Sandeep Sodhi Kakkar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heavy Metal ◽  
Milk Production ◽  
Metabolic Profile ◽  
Antioxidant Status ◽  
Live Cells ◽  
Metal Exposure ◽  
Heavy Metal Exposure ◽  
Exposed Area ◽  
Oral Supplementation

Background: Oxidative stress is a unified concept for the assessment of metabolic status of buffaloes affected by a cocktail of heavy metal exposure from environment and it may significantly affect the metabolic profile and milk production during summer season. The present study aimed at evaluation of effect of probiotic (Saccharomyces cerevisiae strain 1026) and amla powder (Embilica officinalis) supplementation on levels of heavy metals, antioxidant status, oxidative stress in lymphocytes, metabolic profile and milk production of summer stressed buffaloes exposed to environmental heavy metal pollution. Methods: Twenty summer stressed lactating Murrah buffaloes of the same age group, close parity and in early lactation from the heavy metal exposed area were divided into two groups: Control (without supplementation) and Treatment group (supplemented with Saccharomyces cerevisiae1026) @ 50 billion live cells / animal /day and Amla powder @ 86 mg/kg body weight for 30 days. Blood and milk samples were collected from both the groups on the day of starting of supplementation (i.e. day 0) and on there after days 15 and 30 and analyzed for antioxidant status, metabolic profile, milk yield and quality.Result: Oral supplementation of probiotic and amla powder for 30 days improved the antioxidant status, metabolic profile and milk production of summer stressed buffaloes residing in heavy metal exposed area. The percentage monetary gains from buffaloes fed probiotic and amla powder was 13.68% more as compared to control.

Oxidative stress-induced alterations in seminal plasma antioxidants: Is there any association with keap1 gene methylation in human spermatozoa?

Andrologia ◽  
2018 ◽  
Vol 51 (1) ◽  
pp. e13159 ◽  
Author(s):  
Mahsa Darbandi ◽  
Sara Darbandi ◽  
Ashok Agarwal ◽  
Saradha Baskaran ◽  
Pallav Sengupta ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Seminal Plasma ◽  
Human Spermatozoa ◽  
Gene Methylation

scholarly journals Sex Differences in Age-Associated Type 2 Diabetes in Rats—Role of Estrogens and Oxidative Stress

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Ana Díaz ◽  
Raúl López-Grueso ◽  
Juan Gambini ◽  
Daniel Monleón ◽  
Cristina Mas-Bargues ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
Replacement Therapy ◽  
Metabolic Profile ◽  
Estrogen Replacement Therapy ◽  
Diabetic Rats ◽  
Estrogen Replacement ◽  
Antioxidant Genes

Females live longer than males, and the estrogens are one of the reasons for this difference. We reported some years ago that estrogens are able to protect rats against oxidative stress, by inducing antioxidant genes. Type 2 diabetes is an age-associated disease in which oxidative stress is involved, and moreover, some studies show that the prevalence is higher in men than in women, and therefore there are sex-associated differences. Thus, the aim of this study was to evaluate the role of estrogens in protecting against oxidative stress in type 2 diabetic males and females. For this purpose, we used Goto-Kakizaki rats, which develop type 2 diabetes with age. We found that female diabetic rats showed lower glycaemia levels with age than did diabetic males and that estrogens enhanced insulin sensitivity in diabetic females. Moreover, glucose uptake, measured by positron emission tomography, was higher in the female brain, cerebellum, and heart than in those from male diabetic rats. There were also sex-associated differences in the plasma metabolic profile as determined by metabolomics. The metabolic profile was similar between estrogen-replaced and control diabetic rats and different from ovariectomized diabetic rats. Oxidative stress is involved in these differences. We showed that hepatic mitochondria from females produced less hydrogen peroxide levels and exhibited lower xanthine oxidase activity. We also found that hepatic mitochondrial glutathione oxidation and lipid oxidation levels were lower in diabetic females when compared with diabetic males. Ovariectomy induced oxidative stress, and estrogen replacement therapy prevented it. These findings provide evidence for estrogen beneficial effects in type 2 diabetes and should be considered when prescribing estrogen replacement therapy to menopausal women.

scholarly journals BsmI polymorphism in the vitamin D receptor gene is associated with 25-hydroxy vitamin D levels in individuals with cognitive decline

2018 ◽  
Vol 76 (11) ◽  
pp. 760-766 ◽  
Author(s):  
Ana Carolina R. de Oliveira ◽  
Carolina A. Magalhães ◽  
Cristina M. G. Loures ◽  
Vanessa G. Fraga ◽  
Leonardo C. de Souza ◽  
...  
Keyword(s):  
Vitamin D ◽  
Cognitive Decline ◽  
Vitamin D Receptor ◽  
Gene Polymorphisms ◽  
Vdr Gene ◽  
Bsmi Polymorphism ◽  
Vitamin D Levels ◽  
Vdr Gene Polymorphisms ◽  
Significant Difference ◽  
25 Hydroxy Vitamin D

ABSTRACT Elderly people are at a high risk of developing vitamin D (VitD) deficiency due to both decreased intake and cutaneous synthesis. Most of the biological actions of VitD are mediated by the vitamin D receptor (VDR), which is present in neurons and glial cells of the hippocampus, and in the cortex and subcortical nuclei, essential areas for cognition. It is known that VDR gene polymorphisms may decrease the VDR affinity for VitD. Objective: The present study aimed to investigate the influence of VitD levels on cognitive decline in patients with dementia due to Alzheimer's disease (AD, n = 32) and mild cognitive impairment (MCI, n = 15) compared to cognitively healthy elderly (n = 24). We also evaluated the association of VDR gene polymorphisms with cognitive disturbance. Methods: Four polymorphisms on the VDR gene were studied, namely, BsmI, ApaI, FokI and TaqI, by polymerase chain reaction-restriction fragment length polymorphism. Serum levels of 25-hydroxy vitamin D (25(OH)D) were determined by high performance liquid chromatography. Results: No significant difference in 25(OH)D levels or genotypic/allelic frequencies was observed between the groups. Deficiency of 25(OH)D was more frequently observed in women. The AA/AG genotypes of the BsmI polymorphism was associated with sufficient 25(OH)D levels, while the GG genotype of this same polymorphism was associated to insufficient levels in the cognitively-impaired group (individuals with AD or MCI). Conclusions: The data obtained do not confirm the relationship between reductions of VitD levels, polymorphisms in the VDR gene, and altered cognitive function in this sample. However, the data indicate that BsmI polymorphism in the VDR gene is associated with the VitD levels in individuals with cognitive decline.

scholarly journals VDR gene methylation as a molecular adaption to light exposure: Historic, recent and genetic influences

2017 ◽  
Vol 29 (5) ◽  
pp. e23010 ◽  
Author(s):  
Emma L Beckett ◽  
Patrice Jones ◽  
Martin Veysey ◽  
Konsta Duesing ◽  
Charlotte Martin ◽  
...  
Keyword(s):  
Light Exposure ◽  
Genetic Influences ◽  
Gene Methylation ◽  
Vdr Gene

A Methylation Profile of Tumor Suppressor Genes Predicts a Poorer Survival in Patients with Refractory Anemia with Ringed Sideroblasts.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 2780-2780
Author(s):  
Ana Valencia ◽  
Jose Cervera ◽  
Esperanza Such ◽  
Esther Gamero ◽  
Mariam Ibañez ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Tumor Suppressor Genes ◽  
Methylation Status ◽  
Methylation Profile ◽  
Refractory Anemia ◽  
Aberrant Methylation ◽  
Gene Methylation ◽  
Suppressor Genes ◽  
Ring Sideroblasts ◽  
Aberrant Gene

Abstract Abstract 2780 Poster Board II-756 Patients with refractory anemia with ring sideroblasts (RARS) are considered to have good prognosis and anemia is usually managed with best supportive care and erythroid stimulating agents. Nowadays, no specific molecular marker related to outcome and disease progression has been identified. Several genes involved in cell cycle and apoptosis that may become inactivated by aberrant hypermethylation have been identified in patients with myelodysplastic syndromes (MDS) but the significance of a methylation profile (studying several genes at the same time) in RARS is unknown, mainly because the number of patients with RARS in published reports is rather low. To assess the implication of aberrant methylation in RARS, we studied the methylation status of 25 sequences of a set of tumor suppressor genes in 40 patients (median age 70 yr, 25 male gender) with RARS according to FAB criteria [WHO classification, RARS in 22 patients (55%); refractory citopenia with multilineage dysplasia and ring sideroblasts, 18 (45%)] by means of methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) assay. The MS-MLPA procedure (SALSA MLPA kit ME001, MRC-Holland, Amsterdam, The Netherlands) has been developed for detecting in a semi-quantitative manner changes in the methylation status of 25 tumor suppressor genes in a single experiment. Briefly, approximately 50 ng of DNA were denatured and hybridized with MLPA probes. Subsequently, the probes were ligated in half of every sample, whereas for the rest of the sample, ligation was combined with an endonuclease HhaI digestion resulting in ligation of the methylated sequences only. PCR was performed as described by the manufacturer, and then separated by capillary gel electrophoresis and quantified using the Genemapper software (ABI 310, Applied Biosystems, Foster City, CA). Quantification of the methylation status was done by dividing the peak area with the combined areas of the control probes lacking the target sequence of the HhaI. Finally, the relative peak area of each target probe from the digested sample was compared with those obtained from the undigested sample. Aberrant methylation was scored when the calculated methylation percentage was >10%. To validate the MS-MLPA method the methylation status of CDKN2B was also analyzed by methylation-specific PCR (MSP). Actuarial curves of OS were built by Kaplan-Meier method and differences between curves compared with log-rank tests. Small numbers precluded the use of multivariate methodology. The MS-MLPA analysis detected methylation of at least one gene in 17 patients (42.5%). The genes aberrantly methylated were CDKN2B (n = 8, 20%), RASSF1 (n = 7, 17%), RARB (n = 3, 7.5%), CDH13 (n = 3, 7.5%) and FHIT (n = 2; 5%). Of the 17 patients, five (12%) presented methylation in two genes (RASSF1-FHIT in 2, RASSF1-RARB in 1, RASSF1-CDH13 in 1, and CDKN2B-CDH13 in 1, who was the only patient who progressed to AML). The presence of aberrant gene methylation was not significantly associated with any clinical or biological characteristic or WHO morphological subtype. Patients with aberrant gene methylation had a significantly shorter overall survival (OS) than patients without methylated genes (median OS, 72 mo vs 114 mo, respectively; P = 0.03). Patients with hemoglobin level below 10 g/dL and platelet count below 150 ×109/L also had a significantly poorer OS (P= 0.01 and P= 0.02, respectively). As the majority of probes used in the MS-MPLA method detect methylation in only one CpG pair, the results of CDKN2B methylation were validated by MSP, which yielded the same methylation results than with MS-MPLA methodology. The 8 patients with methylated CDKN2B show a trend for a shorter survival than the remaining 32 with unmethylated CDKN2B (median 72 mo vs 114 mo, P = 0.08). The results of this study indicate that aberrant methylation of several tumor suppressor genes is observed in a substantial proportion of patients with RARS. As occurs in patients with high-risk MDS, our results suggest that aberrant gene methylation confers a poor prognosis in RARS, but these data and their potential independent value require confirmation in larger series that employ multivariate methods. Finally, these findings provide a strong rationale for the use of hypomethylating agents (e.g., azacitidine or decitabine) in patients with RARS. This work has been partially supported by ISCIII grants RD06/0020/0031 and CA08/00141. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Serum vitamin D and vitamin D receptor gene polymorphism in Moroccan patients with systemic lupus erythematosus

2017 ◽  
Vol 5 (3) ◽  
pp. 811
Author(s):  
Naoual El Omri ◽  
Fadwa Mekouar ◽  
Youssef Sekkach ◽  
Mohamed Jira ◽  
Mohamed El Qatni ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Receptor ◽  
Clinical Manifestations ◽  
Serum Vitamin ◽  
Systemic Lupus ◽  
Vdr Gene ◽  
Bsmi Polymorphism ◽  
Serum Vitamin D ◽  
Vdr Gene Polymorphisms ◽  
Moroccan Patients

Background: Vitamin D plays an important role in the immunomodulation and could be involved in the development of autoimmune diseases such as systemic lupus erythematous (SLE). The study of the polymorphism of the Vitamin D Receptor (VDR) gene may be of interest in explaining the pathophysiology of SLE.Methods: In this study, we aimed to examine the characteristics of VDR gene BsmI polymorphism for the first time in Moroccan patients with SLE and their relationship with clinical manifestations of the disease. We also measured the serum level of 25-hyroxyvitamin D3 to assess its relation to such polymorphism.Results: The study included 66 SLE patients and 91 healthy controls. Our results showed that there were no differences observed in VDR genotypes and allelic distribution within the two groups. Both groups were in Hardy-Weinberg equilibrium, with no significant P values for the observed and expected genotype frequencies. 25-hyroxyvitamin D3 serum levels were the same in the two groups.Conclusions: Based on the results of the present study. We cannot verify any association between VDR gene BsmI polymorphism and SLE. This polymorphism could not be regarded as a genetic marker of the SLE. A larger study examining BsmI and other VDR gene polymorphisms is needed.

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