scholarly journals In vivo assessment and characterization of lactic acid bacteria with probiotic profile isolated from human milk powder

2020 ◽  
Author(s):  
Blanca Rosa Aguilar Uscanga ◽  
Ariana Rodríguez Arreola ◽  
Josué R. Solís Pacheco ◽  
Monique Lacroix ◽  
Edgar Balcazar López ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Human Milk ◽  
Milk Powder ◽  
In Vivo Assessment

scholarly journals Isolation of lactic acid bacteria from swine milk and characterization of potential probiotic strains with antagonistic effects against swine-associated gastrointestinal pathogens

2016 ◽  
Vol 62 (6) ◽  
pp. 514-524 ◽  
Author(s):  
Sandra Rayén Quilodrán-Vega ◽  
Julio Villena ◽  
José Valdebenito ◽  
María José Salas ◽  
Cristian Parra ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Human Milk ◽  
Salmonella Enterica ◽  
Enterohemorrhagic Escherichia Coli ◽  
Salmonella Infection ◽  
Antagonistic Effects ◽  
Gastrointestinal Pathogens

Probiotics are usually isolated from the gastrointestinal tract of humans and animals. The search of probiotics in human milk is a recent field of research, as the existence of the human milk microbiome was discovered only about a decade ago. To our knowledge, no reports regarding the potential probiotic effect of bacteria from swine milk have been published. In this work, we isolated several lactic acid bacteria from swine milk and evaluated them for them potential as probiotics. Among the isolated strains, Lactobacillus curvatus TUCO-5E showed antagonistic effects against swine-associated gastrointestinal pathogens. TUCO-5E was able to reduce the growth of enterotoxigenic and enterohemorrhagic Escherichia coli strains as well as pathogenic salmonella. In vitro exclusion and displacement assays in intestinal epithelial cells showed a remarkable antagonistic effect for L. curvatus TUCO-5E against Salmonella sp. strain TUCO-I7 and Salmonella enterica ATCC 13096. Moreover, by using a mouse model of Salmonella infection, we were able to demonstrate that preventative administration of L. curvatus TUCO-5E for 5 consecutive days was capable of decreasing the number of Salmonella enterica serovar Typhimurium in the liver and spleen of treated mice, compared with the controls, and prevented dissemination of the pathogen to the blood stream. Therefore, we have demonstrated here that swine milk is an interesting source of beneficial bacteria. In addition, the results of this work suggest that L. curvatus TUCO-5E is a good candidate to study in vivo the protective effect of probiotics against intestinal infection and damage induced by Salmonella infection in the porcine host.

Selection and characterization of potential probiotic Lactobacilli spp isolated from chicken feces may be used as a potent antibacterial agent

2016 ◽  
Vol 35 (1) ◽  
Author(s):  
Nazila Aazami ◽  
Enayatollah Kalantar ◽  
Helen Poormazaheri ◽  
Nassimeh Setayesh vali Pour ◽  
Gholamreza Salehi Jouzan
Keyword(s):  
Antibacterial Activity ◽  
Antibiotic Resistance ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Acid Tolerance ◽  
Resistance Test ◽  
Effect Of Temperature ◽  
Rdna Sequencing

<italic>Lactobacillus</italic> strains commonly used as probiotics which possess some properties such as resistance to gastric acidity and bile salts, production of antimicrobial compounds, the ability to modulate immune responses, and adhesion to gut tissues. This study aimed to isolate, identify, production and characterization of the probiotic strains. Total of 10 digestive tracts of chickens fed without antibiotics for presence of <italic>Lactobacillus spp</italic> was evaluated. All isolates were grown with different conditions like effect of temperature, pH, pepsine, bile, sodium chloride tolerance, aggregation, adhesion and antibiotic resistance test tested and then antibacterial activity was determined.In this study, 34 lactic acid bacteria were investigated as putative probiotic candidates. Based on the molecular identification using 16S rDNA sequencing the isolates belonged to <italic>Lactobacillus</italic> species. The strains showed potent antibacterial activity. The results on acid tolerance showed that all the strains tested survived at pH 2.0 to 5.5. <italic>L. agilis</italic> strain M18, <italic>L. salivarius</italic> strain M10, <italic>L.vaginalis</italic> strains M8 and M19, and <italic>L. crispatus</italic> strains Or2 and M10 showed the lowest sensitivity to pepsine. All the studied Lactobacillus strains were able to grow at both 15 and 45°C . Five Lactobacillus strains adhered well to the Caco-2 cells. The result of antibiotic resistance test of 34 lactic acid bacteria showed that most of them were resistant or intermediate. Based on 34 <italic>Lactobacillus</italic> strains, <italic>L. vaginalis</italic> strain M8 and <italic>L. reuteri</italic> strain M6 were selected for the further in vivo assays and possible eventual use as prophylactic and therapeutic agent.

scholarly journals Isolation and Characterization of Lactic Acid Bacteria from Human Milk and Newborn Feces

2016 ◽  
Vol 10 (4) ◽  
pp. 2613-2620 ◽  
Author(s):  
Ikram Medjaoui ◽  
Bouabdellah Rahmani ◽  
Malika Talhi ◽  
Fatima Mahammi ◽  
Fatima Moghtit ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Human Milk ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of lactic acid bacteria from human milk

2020 ◽  
Vol 103 (11) ◽  
pp. 9980-9991
Author(s):  
Wenli Kang ◽  
Lina Pan ◽  
Can Peng ◽  
Ling Dong ◽  
Sufang Cao ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Human Milk ◽  
Isolation And Characterization

scholarly journals Probiotic characterization of lactic acid bacteria isolated from human milk

2020 ◽  
Vol 9 (7) ◽  
pp. 383-388
Author(s):  
Jithu Rappai ◽  
AK Beena ◽  
Ligimol James ◽  
Aparna Sudhakaran V
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Human Milk

scholarly journals Characterization of Bacteriocin Produced by Lactic Acid Bacteria Isolated from Solid Waste of Soymilk production

2021 ◽  
Vol 709 (1) ◽  
pp. 012020
Author(s):  
Evy Rossi ◽  
Akhyar Ali ◽  
Raswen Efendi ◽  
Fajar Restuhadi ◽  
Yelmira Zalfiatri ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Solid Waste

scholarly journals Identification and characterization of lactic acid bacteria isolated from traditional pickles in Sichuan, China

2012 ◽  
Vol 58 (3) ◽  
pp. 163-172 ◽  
Author(s):  
Jie Yu ◽  
Wa Gao ◽  
Manjun Qing ◽  
Zhihong Sun ◽  
Weihong Wang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Identification And Characterization

scholarly journals Characterization of macrophage-activating lactic acid bacteria isolated from Mukeunji

2016 ◽  
Vol 25 (2) ◽  
pp. 595-599 ◽  
Author(s):  
Jae-Hwan Kim ◽  
Jingmei Li ◽  
Seon-Kyeong Han ◽  
Pei Qin ◽  
Jushin Kim ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria
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