VALIDATED CAPILLARY ZONE ELECTROPHORESIS APPROACH FOR SIMULTANEOUS SEPARATION AND DETERMINATION OF HEPATITIS C SOFOSBUVIR AND LEDIPASVIR IN TABLET DOSAGE FORM

2017 ◽  
pp. 129-147 ◽  
Author(s):  
Maha F. El-Tohamy
Keyword(s):  
Hepatitis C ◽  
Capillary Zone Electrophoresis ◽  
Dosage Form ◽  
Simultaneous Separation ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Tablet Dosage

Simultaneous Separation and Determination of Aconitine Compounds in Radix aconiti praeparata by High Performance Capillary Zone Electrophoresis

2014 ◽  
Vol 884-885 ◽  
pp. 522-525
Author(s):  
Su Ya Gao ◽  
Li Wang ◽  
Yun Zhang ◽  
Hua Li
Keyword(s):  
Capillary Zone Electrophoresis ◽  
High Performance ◽  
Uv Detection ◽  
Beta Cyclodextrin ◽  
Simultaneous Separation ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Radix Aconiti ◽  
Optimized Conditions

A simple and inexpensive high performance capillary zone electrophoresis (CZE) was applied to separate and determine three alkaloids in Radix Aconiti Praeparata simultaneously. The optimized conditions for separation were obtained using 20 mmol/L borax buffer (pH 9.0) containing 2 mmol/L beta-cyclodextrin (β-CD) and 20% methanol. Online UV detection was performed at 235 nm. A voltage of 16 kV was carried at 25°C. Injection was performed at 0.5psi for 10 s. The separation and determination were satisfactory and quick.

scholarly journals Simultaneous Separation of 14 Antiarrhythmic Drugs and Determination of Mexiletine and Flecainide by Capillary Zone Electrophoresis

2007 ◽  
Vol 90 (4) ◽  
pp. 977-986 ◽  
Author(s):  
Rafal Pietra ◽  
Dorota Kowalczuk ◽  
Hanna Hopkala
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Applied Voltage ◽  
Antiarrhythmic Drugs ◽  
Fused Silica ◽  
Effective Length ◽  
Relative Standard ◽  
Simultaneous Separation ◽  
Zone Electrophoresis ◽  
Capillary Zone

Abstract A method using capillary zone electrophoresis was developed for the simultaneous separation of 14 antiarrhythmic drugs belonging to various classes. The drugs are separated on a fused-silica capillary, 90 cm 75 m (72 cm effective length), with phosphate and acetate buffers as background electrolytes and UV detection at 217 nm. The effects of buffer pH, temperature, and applied voltage on the migration of the drugs were studied. The pH was found to be the most significant factor determining effective separation. The antiarrhythmic compounds are completely separated within a relatively short time (<7 min) by using 70 mM phosphate buffer at pH 7.91, an applied voltage of 28 kV, and a temperature of 32C. Mexiletine (MEX) and flecainide (FLE) were quantified under conditions of the optimum separation. The calibration graphs were constructed over the concentration range of 4.014.0 g/mL for both drugs with good correlation (r 0.9999). Detection and quantitation limits were found to be 0.5 and 1.5 g/mL for FLE and 0.7 and 2.1 g/mL for MEX, respectively. The proposed method was used for the determination of both drugs in their commercial forms with satisfactory precision (relative standard deviations of 0.361.21% for FLE and 0.781.66% for MEX) and accuracy (relative standard errors of 0.131.17% for FLE and 0.351.18% for MEX).

Determination of puerarin in pharmaceutical and biological samples by capillary zone electrophoresis with UV detection

Talanta ◽  
2012 ◽  
Vol 91 ◽  
pp. 83-87 ◽  
Author(s):  
Lizhen Liu ◽  
Feng Feng ◽  
Shaomin Shuang ◽  
Yunfeng Bai ◽  
Martin M.F. Choi
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Biological Samples ◽  
Uv Detection ◽  
Zone Electrophoresis ◽  
Capillary Zone
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