An effective Bio banking strategy for Indian Native chicken: Kadaknath through Primordial Germ Cells cryopreservation and Gonadal transplantation

2021 ◽  
Author(s):  
ADNAN NAIM ◽  
SURYAKANT MISHRA
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Native Chicken ◽  
Banking Strategy

scholarly journals Restoration of Genetic Resources from Ehime Native Chicken via Transferred Primordial Germ Cells (PGCs)

2001 ◽  
Vol 38 (4) ◽  
pp. 302-307 ◽  
Author(s):  
Hiroki Furuta ◽  
Keiji Kinoshita ◽  
Yoshizane Maeda ◽  
Noboru Fujihara
Keyword(s):  
Genetic Resources ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Native Chicken

63 THE EFFECT OF A MODIFIED CRYOPRESERVATION METHOD ON THE VIABILITY OF FROZEN–THAWED PRIMORDIAL GERM CELL ON THE KOREAN NATIVE CHICKEN (Ogye)

2014 ◽  
Vol 26 (1) ◽  
pp. 145
Author(s):  
H. Kim ◽  
D. H. Kim ◽  
J. Y. Han ◽  
S. B. Choi ◽  
Y.-G. Ko ◽  
...  
Keyword(s):  
Germ Cells ◽  
Germ Line ◽  
New Technology ◽  
Primordial Germ Cells ◽  
Primordial Germ Cell ◽  
Genetic Material ◽  
Trypan Blue Exclusion ◽  
Trypan Blue Exclusion Method ◽  
Native Chicken ◽  
Korean Native Chicken

Cryopreservation of poultry semen has been reported, but preservation of female genetic material has not been possible because of the unique anatomical and physiological characteristics of the avian egg. Thus, conservation of genetic material in chickens was attempted by preserving primordial germ cells (PGC) in LN2. This study established a method for preserving chicken PGC that enables long-term storage in LN2 for preservation of species. The purpose of this study was to clarify the effects of fetal bovine serum (FBS) or chicken serum (CS) treatment on the viability of cryopreserved PGC in the Korean native chicken (Ogye). Primordial germ cells were separated from a germinal gonad using a fine glass micropipette under a microscope and were suspended in a freezing medium containing freezing and protecting agents [e.g. dimethyl sulfoxide (DMSO) and ethylene glycol (EG)]. The PGC were then purified using the magnetic-activated cell sorting (MACS) method. The viability of the PGC in both groups was determined by the trypan blue exclusion method. The values of the 0, 5, 10, and 15% DMSO plus FBS treatment were 21.6, 30.36, 36.42, 50.39, and 48.36%, respectively. The viability of PGC after freeze-thawing was significantly higher for the 10% EG plus FBS treatment than for the 10% EG plus CS treatment (P < 0.05; 64.36 v. 50.66%). This study established a method for preserving chicken PGC that enables systematic storage and labelling of cryopreserved PGC in LN2 at a germplasm repository and ease of entry into a database. In the future, the importance of this new technology is that poultry lines can be conserved while work is being conducted on improving the production of germ line chimeras.

scholarly journals The Evaluation of Various Conditions in the Cryopreservation of Primordial Germ Cells on Korean Native Chicken (Ogye)

2014 ◽  
Vol 41 (4) ◽  
pp. 249-259
Author(s):  
Hyun Kim ◽  
Young Moo Cho ◽  
Jae Yong Han ◽  
Sung Bok Choi ◽  
Mi Jeong Byun ◽  
...  
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Native Chicken ◽  
Korean Native Chicken

scholarly journals Successful Cryopreservation and Regeneration of a Partridge Coloured Hungarian Native Chicken Breed Using Primordial Germ Cells

2021 ◽  
pp. 101207
Author(s):  
Bence Lázár ◽  
Mariann Molnár ◽  
Nikoletta Sztán ◽  
Barbara Végi ◽  
Árpád Drobnyák ◽  
...  
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Chicken Breed ◽  
Native Chicken

scholarly journals Cryopreservation of Primordial Germ Cells(PGCs) from Korean Native Chicken(Ogye) Embryos using Commercial Cryoprotectants

2013 ◽  
Vol 40 (3) ◽  
pp. 163-169
Author(s):  
Hyun Kim ◽  
Dong Hun Kim ◽  
Jae Yong Han ◽  
Yoon Jung Do ◽  
Jae Hwan Kim ◽  
...  
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Native Chicken ◽  
Korean Native Chicken

Hexachlorobenzene toxicity in the rat ovary: II. Ultrastructure induced by medium (10 mg/kg) dose exposure

1992 ◽  
Vol 50 (1) ◽  
pp. 668-669
Author(s):  
Amreek Singh ◽  
Warren G. Foster ◽  
Anna Dykeman ◽  
David C. Villeneuve
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Surface Epithelium ◽  
Morphological Parameters ◽  
Comprehensive Investigation ◽  
Ovary Surface Epithelium ◽  
Rat Ovary ◽  
High Doses

Hexachlorobenzene (HCB) is a known toxicant that is found in the environment as a by-product during manufacture of certain pesticides. This chlorinated chemical has been isolated from many tissues including ovary. When administered in high doses, HCB causes degeneration of primordial germ cells and ovary surface epithelium in sub-human primates. A purpose of this experiment was to determine a no-effect dose of the chemical on the rat ovary. The study is part of a comprehensive investigation on the effects of the compound on the biochemical, hematological, and morphological parameters in the monkey and rat.

Transfusion of Chick Primordial Germ Cells into Quail Embryos and their Settlement in Gonads

1998 ◽  
Vol 69 (10) ◽  
pp. 911-915 ◽  
Author(s):  
Tamao ONO ◽  
Ryohei YOKOI ◽  
Seishi MAEDA ◽  
Takao NISHIDA ◽  
Hirohiko AOYAMA
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells
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