scholarly journals The Effect of Turmeric Rhizome (Curcuma Longa Linn)On Decreasing Non-Alcoholic Steatohepatitis in Deep Frying Oil Induced Male Wistar Rats

2018 ◽  
Vol 11 (4) ◽  
pp. 361-367
Author(s):  
Karmila Kaban ◽  
Sunarti
Keyword(s):  
Wistar Rats ◽  
Curcuma Longa ◽  
Frying Oil ◽  
Deep Frying ◽  
Alcoholic Steatohepatitis ◽  
Male Wistar Rats

scholarly journals Hepatoprotective effect of red ginger rhizome extract in deep frying oil-fed male wistar rats

2017 ◽  
Vol 36 (3) ◽  
pp. 228 ◽  
Author(s):  
Sunarti Sunarti ◽  
Edy Fachrial ◽  
Urip Harahap ◽  
Delyuzar Delyuzar ◽  
Tri Widyawati ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Wistar Rats ◽  
Fatty Degeneration ◽  
Frying Oil ◽  
Deep Frying ◽  
Treatment Groups ◽  
Whitney Test ◽  
Ginger Rhizome ◽  
Male Wistar Rats ◽  
Red Ginger

BACKGROUND<br />Non-alcoholic steatohepatitis (NASH) is a chronic liver disease associated with increased free fatty acids, trans fatty acids and free radical compounds due to the consumption of deep frying oil (DFO). Ginger has hypolipidemic and antioxidant properties, and acts as an insulin sensitizer. The present study was conducted to evaluate the hepatoprotective effect of an ethanol extract of Zingiber officinale Roxb var rubrum (EEZO) in DFO-fed male Wistar rats.<br /><br />METHODS<br />A study of experimental design was conducted involving 24 non-alcoholic steatohepatitis (NASH) male Wistar rats that were randomly divided into 4 groups. Group K1 (control) was given DFO-3 (3 times heated DFO) at a dose of 10 ìl/g BW/day. The treatment groups (P1, P2, P3) were given DFO-3 and red ginger rhizome extract at doses of 100, 200, and 400 mg/kg BW orally for 30 days. The histopathology examination assessed fatty degeneration, and degree of fibrosis. The data were analyzed with the Kruskal-Wallis and Mann-whitney test.<br /><br />RESULTS    <br />The Kruskal-Wallis test showed that fatty degeneration (steatosis, lobular inflammation, hepatocyte ballooning and NASH) were significantly different among the four groups (p&lt;0.05). Mann-Whitney test proved that fatty degeneration was not significantly different between control and treatment groups (p&gt;0.05).<br /><br />CONCLUSION<br />Deep frying oil increased fatty degeneration and NASH, and none of red ginger rhizome extract doses was able to inhibit fatty degeneration significantly. For the future research it is intended to prolong the induction time and the administration of red ginger rhizome extract.

scholarly journals The effects of aerobic exercise training with octopamine supplementation on cardiomyocyte apoptosis induced by deep frying oil: the role of caspase and pro-Caspase 3

2020 ◽  
Author(s):  
Pantea kianmehr ◽  
Mohammad Ali Azarbayjani ◽  
Maghsoud Peeri ◽  
Parvin Farzanegi
Keyword(s):  
Aerobic Exercise ◽  
Caspase 3 ◽  
Treated Group ◽  
Heart Tissue ◽  
Frying Oil ◽  
Aerobic Exercise Training ◽  
Expression Level ◽  
Deep Frying ◽  
Male Wistar Rats ◽  
Apoptotic Effects

Abstract Background: Deep frying is a common cooking method, in which cooking is accompanied by carcinogenic by-products such as acrolein. Acrolein is a toxic byproduct of lipid peroxidation, which has been implicated in pulmonary, cardiac, and neurodegenerative diseases. The aim of this study was to explore the effect of aerobic exercise and octopamine on caspase 3 and pro-caspase 3 expression level in heart tissue of rat exposed deep frying oil.Methods: 30 male wistar rats were divided into 5 groups (n=6 in each) including 1) control (CO), 2) deep frying oil (DFO), 3) deep frying oil + exercise (DFO+EXE), 4) deep frying oil + octopamine (DFO+OCT), and 5) deep frying oil + exercise + octopamine (DFO+EXE+OCT). The apoptotic effects of DFO in heart tissue were examined by TUNEL assay. Masson's trichrome stain used to study cardiomyocytic fibers. Moreover, caspase 3 and pro-caspase 3 genes and proteins expression in all groups was evaluated using quantitative real-time PCR and western blotting method, respectively. Results: Data showed a significant increase in apoptotic cells in the DFO-treated group (P <0.05). In Masson's Trichrome stain analysis demonstrated more cardiomyocytic fibers degradation and aggregation lymphocytic cells in DFO that exercise and OCT signisicantly improve this degradation. Also, the expression level of caspase 3 and pro-caspase 3 was significantly decrease in deep frying oil + exercise + octopamine group (P <0.05). Conclusions: According to the result of the current study, it can be assumed that DFO can lead to programmed cell death via the activation of caspase in heart tissue. However, it seems that aerobic exercise with octopamine supplementation improve heart tissue by significantly decrease expression of caspase 3 and pro-caspase 3 that inhibit apoptosis.

scholarly journals Aerobic exercise and octopamine protect against deep-frying oil-induced cardiomyocyte apoptosis through modulation of caspase and pro-caspase 3

2020 ◽  
Author(s):  
Pantea kianmehr ◽  
Mohammad Ali Azarbayjani ◽  
Maghsoud Peeri ◽  
Parvin Farzanegi
Keyword(s):  
Aerobic Exercise ◽  
Caspase 3 ◽  
Heart Tissue ◽  
Frying Oil ◽  
Deep Frying ◽  
Cooking Method ◽  
Male Wistar Rats ◽  
Apoptotic Effects ◽  
Stain Analysis ◽  
Toxic Byproduct

Abstract Background: Deep-frying is a common cooking method accompanied by production of carcinogenesis substance such as acrolein. Acrolein is a toxic byproduct of lipid peroxidation that is involved in the development of pulmonary, cardiac, and neurodegenerative diseases. This study aimed to explore the effect of aerobic exercise (EXE) and octopamine (OCT) on caspase 3 and pro-caspase 3 expression levels in the heart tissue of rats were fed deep-frying oil (DFO).Methods: 30 male Wistar rats were divided into 5 groups (n=6 in each) including 1) control (CO), 2) DFO, 3) DFO+EXE, 4) DFO+OCT and 5) DFO+EXE+OCT. The apoptotic effects of DFO on heart and cardiomyocytes’ fibers were examined by TUNEL assay and Masson's trichrome staining respectively. Also caspase 3 and pro-caspase 3 genes and proteins expression in all groups were evaluated using quantitative real-time PCR and western blotting method, respectively. Results: Data showed a significant increase in apoptotic cells in the DFO group (P <0.05). Masson's trichrome stain analysis demonstrated that the number of cardiomyocytes' fibers are decreased, and collagen deposition is increased in the DFO group. In comparison, the collagen percentage was significantly reduced in DFO+EXE, DFO +OCT and DFO+EXE+OCT groups. Also, the expression level of caspase 3 and pro-caspase 3 was significantly decreased in DFO+EXE+OCT group (P <0.05). Conclusions: The results of this study show that DFO lead to programmed cell death via the activation of caspases in heart tissue. However, it seems that aerobic exercise with octopamine supplementation improves heart tissue through decreasing in the expression of caspase 3 and pro-caspase 3 that inhibit apoptosis.

scholarly journals Testicular cytoprotective activities of Curcuma longa in STZ-induced diabetic adult male wistar rats

1970 ◽  
Vol 6 (1) ◽  
pp. 905-913
Author(s):  
A.J Olanrewaju ◽  
J.O. Owolabi ◽  
S.Y. Olatunji ◽  
A.B.O. Desalu ◽  
W.C. Amaechi
Keyword(s):  
Oxidative Stress ◽  
Leydig Cells ◽  
Wistar Rats ◽  
Curcuma Longa ◽  
Chow Group ◽  
Reproductive Age ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Histological Structure ◽  
Male Wistar Rats

This study was aimed at investigating the cytoprotective activities of Curcuma longa (Turmeric) on the histological structure of the testes in diabetic male rats. Turmeric is commonly called the golden spice, is used as a spice in cooking and also has a long history of medicinal use, dating back nearly 4000 years to the Vedic culture in India. Its major component is the curcumin that is found to be a natural antioxidant. Diabetes affects large number of young men of reproductive age. It is among a number of disorders associated with oxidative stress. There has been a relationship established between the reduction in glucose load and insulin resistance reduction and oxidative stress in people. Male wistar rats (n=24) were allocated randomly into six groups, 4 in each. Group 1: Control were given only water and standard rat chow; Group 2 and 3: turmeric (100mg/kg) and (25mg/kg) respectively; Group 4: induced with 30mg/kg of streptozotocin (STZ) for 3 days; Group 5: STZ with turmeric (100mg/kg); Group 6: STZ with turmeric (25mg/kg). At the end of the experiment (21 days), the testes were excised for histological analysis. The seminiferous tubules, seminiferous epithelium, Leydig cells were shown to be enhanced in the histoarchitecture of the testes of the treated diabetic groups and did not show any toxicity on the testes in the non-diabetic groups. In conclusion, this study has shown that turmeric does not only possess a non-toxic effect but has cytoprotective effects on the histoarchitecture of the testes in diabetic rats.Keywords: histo-architecture, leydig cells, diabetes, oxidative stress, testes, curcumin.

scholarly journals Aerobic exercise and octopamine protects against deep-frying oil-induced cardiomyocyte apoptosis through modulation of caspase and pro-caspase 3

2020 ◽  
Author(s):  
Pantea kianmehr ◽  
Mohammad Ali Azarbayjani ◽  
Maghsoud Peeri ◽  
Parvin Farzanegi
Keyword(s):  
Aerobic Exercise ◽  
Caspase 3 ◽  
Treated Group ◽  
Heart Tissue ◽  
Frying Oil ◽  
Deep Frying ◽  
Male Wistar Rats ◽  
Apoptotic Effects ◽  
Stain Analysis ◽  
Toxic Byproduct

Abstract Background : Deep-frying is a common cooking method in which cooking is accompanied by carcinogenic byproducts such as acrolein. Acrolein is a toxic byproduct of lipid peroxidation, which has been implicated in pulmonary, cardiac, and neurodegenerative diseases. This study aimed to explore the effect of aerobic exercise and octopamine on caspase 3 and pro-caspase 3 expression levels in the heart tissue of rat exposed deep-frying oil. Methods : 30 male Wistar rats were divided into 5 groups (n=6 in each) including 1) control (CO), 2) deep-frying oil (DFO), 3) deep-frying oil+exercise (DFO+EXE), 4) deep-frying oil+octopamine (DFO+OCT), and 5) deep-frying oil+exercise+octopamine (DFO+EXE+OCT). The apoptotic effects of DFO in heart tissue were examined by TUNEL assay. Masson's trichrome stain used to study cardiomyocytes’ fibers. Moreover, caspase 3 and pro-caspase 3 genes and proteins expression in all groups were evaluated using quantitative real-time PCR and western blotting method, respectively. Results : Data showed a significant increase in apoptotic cells in the DFO-treated group ( P <0.05). Masson's trichrome stain analysis demonstrated that the number of cardiomyocytes' fibers are decreased, and collagen deposition is increased in the DFO group. In comparison, the collagen percentage was significantly reduced in exercise, OCT, and exercise+OCT groups. Also, the expression level of caspase 3 and pro-caspase 3 was significantly decreased in deep-frying oil+exercise+OCT group ( P <0.05). Conclusions : The results of our study show that DFO lead to programmed cell death via the activation of caspase in heart tissue. However, it seems that aerobic exercise with octopamine supplementation improves heart tissue by significantly decrease the expression of caspase 3 and pro-caspase 3 that inhibit apoptosis.

Compensatory depression of arterial pressure and reversal of ECG abnormalities by Annona muricata and Curcuma longa in hypertensive Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Olayinka Ayotunde Oridupa ◽  
Ademola Adetokunbo Oyagbemi ◽  
Olumuyiwa Adejumobi ◽  
Folusho Bolawaye Falade ◽  
Ayobami Deborah Obisesan ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Wistar Rats ◽  
Curcuma Longa ◽  
Annona Muricata ◽  
Hypertensive Rats ◽  
Male Wistar Rats ◽  
Red Cell Indices ◽  
Qrs Duration ◽  
Haematological Changes ◽  
Ecg Abnormalities

Abstract Objectives Increasing hypertension incidence in Sub-Sahara Africa and the current cost of management of the metabolic disorder has necessitated research on medicinal plants employed in African Traditional Medicine for hypertension. Thus, this study evaluated antihypertensive effect of Annona muricata leaves or Curcuma longa rhizomes in experimentally-induced hypertensive male Wistar rats (n=70) which were unilaterally nephrectomized and daily loaded with 1% salt. Cardiovascular and haematological changes, as well as urinalysis were determined. Methods Rats were uninephrectomized and NaCl (1%) included in drinking water for 42 days. Extract-treated hypertensive rats were compared to normotensive, untreated hypertensive and hypertensive rats treated with lisinopril (5 mg/70 kg) or hydrochlorothiazide (12.5 mg/70 kg). A. muricata extract or C. longa extract were administered at 100, 200 or 400 mg/kg. Blood pressure (systolic, diastolic and mean arterial) and electrocardiogram was measured on day 41. Twenty-four-hour urine samples were collected from day 42. Blood samples were collected on day 43 for haematology (PCV, red cell indices, WBC and its differentials, and platelets). Results and Conculsions A. muricata or C. longa extracts caused a decline in elevated blood pressure of hypertensive rats. Heart rate and QT segment reduction coupled with prolonged QRS duration were reversed in extract-treated rats, with significant increases in hemogram parameters indicating increased blood viscosity. Also, leukocyturia, proteinuria and ketonuria with increased urine alkalinity, urobilinogen and specific gravity which are classical indicators of poor prognostic outcomes in hypertension were reversed in extract-treated rats. In conclusion, A. muricata and C. longa have cardioprotective effect with reversal of derangements in haemogram and urinalysis associated with hypertension.

scholarly journals Comparative Study of the Effects of Methanolic Extracts of Spondias mombin Leaves and Curcuma longa Rhizomes on Serum Lipid Profile and Electrolytes in Alloxan Induced Diabetes in Male Wistar Rats

2020 ◽  
pp. 1-9
Author(s):  
Saronee Friday ◽  
Sunday O. Ojeka ◽  
Okekem Amadi ◽  
Ogadinma N. Ilochi ◽  
Datonye V. Dapper
Keyword(s):  
Lipid Profile ◽  
Serum Lipid ◽  
Wistar Rats ◽  
Low Dose ◽  
Curcuma Longa ◽  
Density Lipoprotein ◽  
Serum Lipid Profile ◽  
High Dose ◽  
Spondias Mombin ◽  
Male Wistar Rats

Introduction: Diabetes mellitus is an important risk factor for cardiovascular diseases; the possible uses of Spondias mombin and Curcuma longa rhizomes for the treatment of diabetes and cardiovascular disorders have become prevalent in our environment. Aim: The present study attempts a Comparative assessment of the effects of methanolic extracts of Spondias mombin leaves and Curcuma longa rhizomes on serum lipid profile and electrolytes in alloxan induced diabetes in male wistar rats. Methodology: 90 male wistar rats were randomly divided into 9 groups of 10 rats each. Diabetes was induced intraperitonially using alloxan at 200 mg/kg-bw. The different rat Groups were treated with extracts and glibenclamide orally for 42 days as follows: Group 1: untreated non diabetic; Group 2: untreated diabetic; Group 3: diabetic + low dose Spondias mombin; Group 4: diabetic + high dose Spondias mombin; Group 5: diabetic + low dose Curcuma longa; Group 6: diabetic + high dose Curcuma longa; Group 7: diabetic + low dose combined Spondias mombin and Curcuma longa; Group 8: diabetic + high dose combined Spondias mombin and Curcuma longa; and Group 9; diabetic + glibenclamide. Blood was collected on day 43 by cardiac puncture for determination of serum lipid profile and electrolytes. Results: Compared to Group 2, total serum cholesterol, triglyceride, low density lipoprotein and electrolytes were significantly reduced while high density lipoprotein was significantly increased in all treated Groups (p<0.05). Compared to Groups 3 to 6, Groups 7 and 8 rats showed a significant reduction in total cholesterol, triglyceride and low density lipoprotein as well as electrolytes (p<0.05): however, high density lipoprotein was significantly increased (p<0.05). Conclusion: Spondias mombin showed better hypolipidemic effects compared to Curcuma longa.  However, results show that combined treatment with both extracts had better hypolipidemic effects than administration of individual extracts. Further research is recommended to evaluate the possible mechanism of action of these extract.

Structural and functional renal alterations in five-sixth nephrectomized rats on unrestricted diet

1986 ◽  
Vol 44 ◽  
pp. 342-343
Author(s):  
I. Stachura ◽  
M. Pardo ◽  
J. Costello ◽  
D.M. Landwehr
Keyword(s):  
Wistar Rats ◽  
Renal Damage ◽  
Standard Diet ◽  
Experimental Conditions ◽  
Phosphate Intake ◽  
Dietary Restrictions ◽  
Severe Reduction ◽  
Male Wistar Rats ◽  
Unrestricted Diet ◽  
Progressive Renal Insufficiency

Under experimental conditions severe reduction of renal mass results in the hyperfiltration of the remaining nephrons leading to a progressive renal insufficiency. Similar changes are observed in patients with various renal disorders associated with a loss of the functioning nephrons. The progression of renal damage is accelerated by high protein and phosphate intake, and may be modified by the dietary restrictions.We studied 50 five-sixth nephrectarrized male Wistar rats on a standard diet (Rodent Laboratory Chow 5001 Ralston Purina Co., Richmond, Indiana; containing 23.4% protein) over a 20 week period.

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