scholarly journals Spectrophotometric determination of Clinidipine, Efavirenz, Prazosin drugs by using Folin-Ciocalteu Reagent

2020 ◽  
Vol 13 (3) ◽  
pp. 210-217
Author(s):  
Naveen Reddy Pailla ◽  
Bhaskar Kuthati
Keyword(s):  
Alkaline Medium ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Absorption Maximum ◽  
Ich Guidelines ◽  
Beer's Law ◽  
Beer’S Law

For the determination of three drugs namely Cilnidipine (CDP), Efavirenz (EVZ), Prazosin (PRZ), a simple, fast, selective and accurate spectrophotometric method has been outlined. This method is based on the development of blue coloured chromogen due to reduction of tungstate and/ or molybdate in Folin-Ciocalteu reagent by Drug in alkaline medium. The coloured species has an absorption maximum at 730 nm, 745 nm and 770 nm and the Beer’s law obeys over the concentration range of 12-72 μg/mL, 10-70 μg/mL, 3-21 μg/mL respectively for the drugs CDP, EVZ, PRZ. This method has been validated according to ICH guidelines and applied to the analysis of pharmaceuticals.

Spectrophotometric Determination of Menadione and Menadione Sodium Bisulfite

1982 ◽  
Vol 65 (1) ◽  
pp. 141-143
Author(s):  
Madiha B Sidhom ◽  
Michel E El-Kommos
Keyword(s):  
Alkaline Medium ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Sodium Bisulfite ◽  
Routine Analysis ◽  
Menadione Sodium Bisulfite ◽  
Beer's Law ◽  
Violet Color ◽  
Beer’S Law

Abstract A spectrophotometric method is described for the determination of menadione and menadione sodium bisulfite in bulk and in solution for injection. The method measures the intensity of the violet color (λmax 540 nm) developed when menadione reacts with thiosemicarbazide in alkaline medium. Beer's law is obeyed in the concentration range 4-40 μg/m L (r = 0.9995). The method is simple, sensitive, and particularly suited for routine analysis of official menadione sodium bisulfite injection. Results are comparable with the USP method.

scholarly journals Rapid Spectrophotometric Determination of Phenoxazine

2010 ◽  
Vol 7 (2) ◽  
pp. 1001-1005
Author(s):  
Baghdad Science Journal
Keyword(s):  
Detection Limit ◽  
Spectrophotometric Determination ◽  
Acidic Medium ◽  
Absorption Maximum ◽  
Aqueous Samples ◽  
Economic Method ◽  
Beer's Law ◽  
Beer’S Law ◽  
The Stability

A rapid high sensitive and inexpensive economic method has been developed for the Determination of phenoxazine by using molecular spectrophotometry. The method is based on the oxidation of phenoxazine by potassium (meta)periodate in acidic medium. The oxidation conditions were selected to enhance the sensitivity and the stability of the pink colored species which shows an absorption maximum at 530 nm. The Beer’s law was obeyed for phenoxazine concentration range from 1 to 6 µg mL-1 with 0.003 µg mL-1 detection limit and provided variation coefficients between 0.4 to 1.7 %. This method was successfully applied for the determination of phenoxazine in aqueous samples

scholarly journals Spectrophotometric Determination of Famciclovir and Racecodotril Using 2, 6-Dichloroquinone–Chlorimide

2007 ◽  
Vol 4 (1) ◽  
pp. 50-52 ◽  
Author(s):  
M. Vamsi Krishna ◽  
P. V. Madhavi Latha ◽  
D. Gowri Sankar
Keyword(s):  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Oxidative Coupling ◽  
Pharmaceutical Preparations ◽  
Coupling Product ◽  
Beer's Law ◽  
Beer’S Law ◽  
Sensitive Spectrophotometric Method

A simple, sensitive spectrophotometric method for the determination of Famciclovir and Racecodotril is developed. It is based on the formation of a colored oxidative coupling product between 2,6-dichloroquinone-chlorimide and the drug is described. The method has been extended to pharmaceutical preparations. The absorption maxima and Beer’s law limits for Famciclovir are 500 nm, 20-100 µg/mL and for Racecodotril are 460 nm, 12.5-62.5 µg/mL.

scholarly journals Spectrophotometric Determination of Paracetamol in bulk and Pharmaceutical Preparations

2015 ◽  
Vol 12 (2) ◽  
pp. 317-323
Author(s):  
Baghdad Science Journal
Keyword(s):  
Sodium Hydroxide ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
Coupling Reaction ◽  
Reaction Conditions ◽  
Accuracy And Precision ◽  
Beer's Law ◽  
Beer’S Law

A simple, and rapid spectrophotometric method for the estimation of paracetamol has been developed. The methods is based on diazotisation of 2,4-dichloroaniline followed by a coupling reaction with paracetamol in sodium hydroxide medium. All variables affecting the reaction conditions were carefully studied. Beer's law is obeyed in the concentration range of 4-350 ?gml?1 at 490 nm .The method is successfully employed for the determination of paracetamol in pharmaceutical preparations. No interferes observed in the proposed method. Analytical parameters such as accuracy and precision have been established for the method and evaluated statistically to assess the application of the method.

scholarly journals Development of Method for Extractive Spectrophotometric Determination of Fe(III) with 2-Hydroxy-1-Naphthalene Carboxaldehyde Phenyl Hydrazone as an Analytical Reagent

2013 ◽  
Vol 14 ◽  
pp. 7-12
Author(s):  
Ratnamala P. Sonawane ◽  
R.S. Lokhande ◽  
Utkarsha M. Chavan
Keyword(s):  
Ethyl Acetate ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Molar Absorptivity ◽  
Beer's Law ◽  
Beer’S Law ◽  
Coloured Complex ◽  
Analytical Reagent

A spectrophotometric method has been developed for the determination of Fe(III) using 2-Hydroxy-1-Naphthalene carboxaldehyde phenyl hydrazone as an extractive reagent. The reagent forms a coloured complex, which has been quantitatively extracted into ethyl acetate at pH 6. The method obeys Beer’s law over arrange from 1 to 7 ppm. The Molar absorptivity and Sandell’s sensitivity were 0.31 × 104 L mol-1cm-1 and 0.13 μg cm-2 respectively. The proposed method is very sensitive and selective. The method has been successfully applied to synthetic and commercial samples.

Spectrophotometric Determination of Some Benzene Sulfonamides with 7,7,8,8-Tetracyanoquinodimethane

1989 ◽  
Vol 72 (6) ◽  
pp. 885-889 ◽  
Author(s):  
Abdel-Maboud I Mohamed
Keyword(s):  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Analytical Data ◽  
Pharmaceutical Preparations ◽  
Pure Form ◽  
Wide Range ◽  
Beer's Law ◽  
Beer’S Law ◽  
Good Agreement

Abstract A new spectrophotometric method for the determination of some unsubstituted benzene sulfonamides is presented. The method is based on the interaction of these derivatives with 7,7,8,8-tetracyanoquinodimethane at pH 9.0-9.5 to produce intense blue products. The quantitation of the products was carried out at 578 nm. Beer’s law was obeyed over a wide range of concentrations for all sulfonamide compounds studied. Optimum analytical conditions were determined, and the color produced was stable for at least 90 min at 25 °C. Analytical data for determination of sulfonamide compounds in pure form are presented together with application of the proposed method for analysis of some commercially available pharmaceutical preparations. The results are in good agreement with those obtained by official procedures.

scholarly journals Development of Method for Extractive Spectrophotometric Determination of Cu(II) with 2-Hydroxy-1-Naphthalene Carboxaldehyde Phenyl Hydrazone as an Analytical Reagent

2013 ◽  
Vol 14 ◽  
pp. 1-6 ◽  
Author(s):  
Ratnamala P. Sonawane ◽  
R.S. Lokhande ◽  
Utkarsha M. Chavan
Keyword(s):  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Molar Absorptivity ◽  
Beer's Law ◽  
Beer’S Law ◽  
Coloured Complex ◽  
Analytical Reagent

A spectrophotometric method has been developed for the determination of Cu(II) using 2-Hydroxy-1-Naphthalene carboxaldehyde phenyl hydrazone as an extractive reagent. The reagent forms a coloured complex, which has been quantitatively extracted into n-butanol at pH 9.2. The method obeys Beer’s law over arrange from 1 to 10 ppm. The Molar absorptivity and Sandell’s sensitivity were 0.97 × 104 L mol-1cm-1 and 0.26 µg·cm-2 respectively. The proposed method is very sensitive and selective. The method has been successfully applied to synthetic and commercial samples.

scholarly journals A spectrophotometric method for determination of fluorophore-to-protein ratios in conjugates of the blue fluorophore 7-amino-4-methylcoumarin-3-acetic acid (AMCA)

1990 ◽  
Vol 38 (1) ◽  
pp. 87-94 ◽  
Author(s):  
M W Wessendorf ◽  
S J Tallaksen-Greene ◽  
R M Wohlhueter
Keyword(s):  
Acetic Acid ◽  
Optical Density ◽  
Extinction Coefficient ◽  
Spectrophotometric Method ◽  
Extinction Coefficients ◽  
Beer's Law ◽  
Beer’S Law ◽  
The Given

7-Amino-4-methylcoumarin-3-acetic acid (AMCA) has been found to be a useful fluorophore for immunofluorescence. The present study describes a spectrophotometric method for determining the ratio of moles AMCA to moles protein (or the f/p ratio) in an AMCA-conjugated IgG. The concentration of a substance absorbing light can be determined spectrophotometrically using Beer's Law: Absorbance = Concentration x Extinction coefficient. From Beer's law, one can derive the following formula for determining the f/p ratio of AMCA-IgG conjugates: f/p = (epsilon 280IgG).A350 - (epsilon 350IgG).A280/(epsilon 350AMCA).A280 - (epsilon 280AMCA).A350 where A is the optical density of the conjugate at the given wavelength and epsilon is the extinction coefficient of a substance at the wavelength specified. Using conjugates of model proteins, it was found that the extinction coefficients of the AMCA moiety of AMCA-conjugated protein were 1.90 x 10(4) at 350 nm and 8.29 x 10(3) at 280 nm. Similarly, it was found that the extinction coefficients of swine IgG were 1.56 x 10(3) at 350 nm and 1.26 x 10(5) at 280 nm. Thus, for AMCA-conjugated swine IgG: f/p = (1.26 x 10(5)).A350 - (1.56 x 10(3)).A280/(1.47 x 10(4)).A280 - (6.42 x 10(3)).A350 [corrected]. Based on this formula, the f/p ratios of some AMCA-IgG conjugates useful for immunohistochemistry have been found to range between 6 and 24.

scholarly journals A New Spectrophotometric Method for the Determination of Dithianon in Commercial Formulations and Its Residues in Foodstuffs

1999 ◽  
Vol 82 (6) ◽  
pp. 1298-1302 ◽  
Author(s):  
Balbir C Verma ◽  
Hari K Thakur ◽  
Jasvir Singh ◽  
Devender K Sharma
Keyword(s):  
Spectrophotometric Method ◽  
Apple Fruit ◽  
Titration Method ◽  
Red Color ◽  
Beer's Law ◽  
Photometric Titration ◽  
Beer’S Law ◽  
Formulation Analysis

Abstract A new, simple, and rapid spectrophotometric method for the microdetermination of dithianon, on the basis of its reaction with a dithiocarbamate, is described. The red color, which develops instantaneously when mixing the fungicide with the reagent in acetonitrile, is stable for at least 1 h and is measured at 520 nm. Beer's law is applicable up to 12 μg/mL dithianon concentration. The method has been successfully adapted to the analysis of the fungicide in commercial formulations and its residues on grains and apple (fruit and leaves). A photometric titration method for formulation analysis of the fungicide has also been developed.

scholarly journals Spectrophotometric Determination of Ziram in a Commercial Sample and Wheat by Extraction of Its Copper Dimethyldithiocarbamate Complex into Molten Naphthalene

2002 ◽  
Vol 85 (1) ◽  
pp. 146-148
Author(s):  
Ashok Kumar Malik ◽  
Avasarla L J Rao
Keyword(s):  
Spectrophotometric Determination ◽  
Final Solution ◽  
Wheat Grain ◽  
Reagent Blank ◽  
Commercial Sample ◽  
Dithiocarbamate Complex ◽  
Beer's Law ◽  
Beer’S Law

Abstract A method was developed for the determination of zinc(II) dimethyldithiocarbamate by converting it into the copper(II) dithiocarbamate complex, which is then extracted into molten naphthalene. The absorbance is measured at 430 nm versus a reagent blank. Beer's law is obeyed for concentrations of 0.63 × 10−3 to 17.2 × 10−3 g/L in the final solution. The method is sensitive and was applied to the determination of ziram in a commercial sample and in wheat grain.

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