scholarly journals Determination of Caffeine Content of Indonesia Luwak Coffee (Mongoose Coffee)Using High Performance Liquid Chromatography (HPLC) Analysis

2019 ◽  
Vol 12 (04) ◽  
pp. 43-47
Author(s):  
Rani Rubiyanti
2003 ◽  
Vol 58 (9-10) ◽  
pp. 763-765 ◽  
Author(s):  
Michał Tomczyk ◽  
Jan Gudej

AbstractThe quantitative determination of flavonoid compounds in flowers and leaves of Ficaria verna Huds. (Ranunculaceae) was carried out in different growing seasons of the plant, using Christ-Müller’s method (Polish Pharmacopoeia, 1999) and high performance liquid chromatography (HPLC) analysis after acid hydrolysis. The flavonoid content was much higher in flowers than in leaves.


HortScience ◽  
2002 ◽  
Vol 37 (4) ◽  
pp. 682-685 ◽  
Author(s):  
Kevin A. Lombard ◽  
Emmanuel Geoffriau ◽  
Ellen Peffley

Direct spectrophotometric determination of quercetin content in onions (Allium cepa L.) was investigated as a possible alternative to high-performance liquid chromatography (HPLC) analysis. Quercetin content in five onion varieties was monitored at 362 nm and quantified using simple spectrophotometric and HPLC methods. HPLC revealed that 3,4'-Qdg and 4'-Qmg comprised up to 93% of total flavonol content detected in the studied varieties. These major quercetin conjugates combined (3,4'-Qdg + 4'-Qmg) and total flavonol conjugates quantified by HPLC correlated closely with spectrophotometer values. Correlation coefficients were 0.96 (P < 0.0001) for 3,4'-Qdg + 4'-Qmg and 0.97 (P < 0.0001) for total flavonol conjugates in onion. Simple spectrophotometric procedure proved to be a valid, efficient, and cost-effective method for the quantification of total quercetin in onion. Chemical names used: quercetin-3,4'-O-diglucoside (3,4'-Qdg); quercetin-4'-O-glucoside (4'-Qmg).


2021 ◽  
Vol 37 (3) ◽  
pp. 663-666
Author(s):  
G. M. M. Anwarul Hasan ◽  
Anuj Kumer Das

Caffeine, a phycostimulant is present in several foods and drinks.In the present study, beverages of different brands in Bangladeshi market were analyzed for caffeine by high-performance liquid chromatography (HPLC) using methanol-water (40:60, v/v) as mobile phase. Caffeine content ranged from 16.33 -19.33 mg/can in soft drinks and 45.66-47.33 mg/can in energy drinks respectively. These data indicated that the levels of caffeine in Bangladeshi soft drinks and energy drinks are within the ranges reported from similar products in other countries.


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