scholarly journals The Effect of Manuka Honey on dHL-60 Cytokine, Chemokine, and Matrix-Degrading Enzyme Release under Inflammatory Conditions

Med One ◽  
2019 ◽  
Keyword(s):  
Enzyme Release ◽  
Manuka Honey ◽  
Degrading Enzyme ◽  
Inflammatory Conditions

Membrane Changes in Polymorphonuclear Leukocytes During Ionophore (A23187) - Induced Lysosomal Release

1977 ◽  
Vol 35 ◽  
pp. 482-483
Author(s):  
P.L. Moore ◽  
P.L. Sannes ◽  
H.L. Bank ◽  
S.S. Spicer
Keyword(s):  
Structural Changes ◽  
Calcium Release ◽  
Clear Understanding ◽  
Ionophore A23187 ◽  
Enzyme Release ◽  
Extracellular Medium ◽  
Pmn Leukocytes ◽  
Intracellular Ion Concentrations ◽  
Membrane Changes ◽  
Pmn Leukocyte

It is thought that calcium and/or magnesium may play important roles in polymorphonuclear (PMN) leukocyte functions such as chemotaxis, adhesion and phagocytosis. Yet, a clear understanding of the biological roles of these ions has awaited the development of techniques which permit a selective alteration of intracellular ion concentrations. Recently, treatment of cells with the ionophore A23187 has been used to alter intracellular divalent cation concentrations. This ionophore is a lipid soluble antibiotic produced by Streptomyces chartreusensis that complexes with both calcium and magnesium (3) and is believed to carry these ions across biological membranes (4). Biochemical investigations of human PMN leukocytes demonstrate that cells treated with A23187 and extracellular calcium release their lysosomal enzymes into the extracellular medium without rupturing and releasing their soluble cytoplasmic enzymes (5,6). The aim of the present study and and a companion report (7) was to investigate the structural changes that occur in leukocytes during ionophore-induced lysosomal enzyme release.

Sarcolemmal permeability changes during early myocardial anoxia

1978 ◽  
Vol 36 (2) ◽  
pp. 642-643
Author(s):  
M. Ashraf ◽  
L. Landa ◽  
L. Nimmo ◽  
C. M. Bloor
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Cell Membrane Permeability ◽  
Enzyme Release ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Sarcolemmal Permeability ◽  
Membrane Changes

Following coronary artery occlusion, the myocardial cells lose intracellular enzymes that appear in the serum 3 hrs later. By this time the cells in the ischemic zone have already undergone irreversible changes, and the cell membrane permeability is variably altered in the ischemic cells. At certain stages or intervals the cell membrane changes, allowing release of cytoplasmic enzymes. To correlate the changes in cell membrane permeability with the enzyme release, we used colloidal lanthanum (La+++) as a histological permeability marker in the isolated perfused hearts. The hearts removed from sprague-Dawley rats were perfused with standard Krebs-Henseleit medium gassed with 95% O2 + 5% CO2. The hypoxic medium contained mannitol instead of dextrose and was bubbled with 95% N2 + 5% CO2. The final osmolarity of the medium was 295 M osmol, pH 7. 4.

Chemical and biological investigations of Manuka honey

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
C Bäcker ◽  
K Wende ◽  
U Meyer ◽  
U Lindequist
Keyword(s):  
Manuka Honey

Inflammatory conditions induces a new subset of osteoclasts that prime TNF[alpha]-producing CD4+ T cells

2016 ◽  
Author(s):  
Lidia Ibanez ◽  
Grazia Abou-Ezzi ◽  
Thomas Ciucci ◽  
Vanessa Amiot ◽  
Anna Mansour ◽  
...  
Keyword(s):  
T Cells ◽  
Cd4 T Cells ◽  
Tnf Alpha ◽  
Inflammatory Conditions

Beauveria bassiana xylanase: a biomass degrading enzyme from an entomopathogen

2020 ◽  
Author(s):  
Santhosh Pillai ◽  
AYODEJI EMMANUEL AMOBONYE ◽  
Prashant Bhagwat
Keyword(s):  
Beauveria Bassiana ◽  
Degrading Enzyme
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