scholarly journals Interleukin-7 receptor Thr244Ile gene polymorphism and the risk of systemic lupus erythematosus

2016 ◽  
Vol 85 (3) ◽  
pp. 192 ◽  
Author(s):  
Pawel Piotr Jagodzinski ◽  
Piotr Piotrowski ◽  
Marzena Olesińska
Keyword(s):  
Lupus Erythematosus ◽  
High Resolution Melting ◽  
Melting Curve ◽  
Melting Curve Analysis ◽  
Neurological Manifestations ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Interleukin 7 ◽  
High Resolution Melting Curve ◽  
And Control

Aim. Recently, the IL-7 receptor (IL-7R) C>T (rs6897932) single nucleotide polymorphism (SNP), which causes a Thr244Ile substitution in the IL-7R α‑chain, has been suggested as a risk factor for SLE.Material and Methods. Using high‑resolution melting curve analysis we studied the distribution of the IL-7R C>T polymorphism in SLE patients (n = 281) and control subjects (n = 541) in the Polish population.Results. We did not find significant differences in the distribution of the IL-7R C>T genotype and alleles between SLE patients and controls. However, in the dominant model (T/T and C/T vs C/C genotypes), we observed a protective effect of the IL-7R C>T polymorphism against the presence of neurological manifestations of SLE [OR = 0.3631 (95% CI = 0.1895–0.6954), p = 0.0017, pcorr = 0.0323] and the presence of anti‑Scl-70 antibodies (Ab) [OR = 0.3141 (95% CI = 0.1503–0.6561), p = 0.0014, pcorr = 0.0266].Conclusion. Our studies suggest that the IL-7R C>T (rs6897932) polymorphism might be involved in the neurological manifestations and the presence of anti‑Scl-70 Abs in patients with SLE.

scholarly journals Sub-7-second genotyping of single-nucleotide polymorphism by high-resolution melting curve analysis on a thermal digital microfluidic device

Lab on a Chip ◽  
2016 ◽  
Vol 16 (4) ◽  
pp. 743-752 ◽  
Author(s):  
Tianlan Chen ◽  
Yanwei Jia ◽  
Cheng Dong ◽  
Jie Gao ◽  
Pui-In Mak ◽  
...  
Keyword(s):  
High Resolution Melting ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Dna Melting ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
High Resolution Melting Curve ◽  
Digital Microfluidic ◽  
Multiple Samples

A novel thermal digital microfluidic (T-DMF) device enables precise thermal modulation and pipelined measurement of multiple samples. Ultrafast DNA melting curve analysis is achieved in less than 7 seconds, with the resolution adequate for single-nucleotide discrimination.

One-shot high-resolution melting curve analysis for KRAS point-mutation discrimination on a digital microfluidics platform

Lab on a Chip ◽  
2022 ◽  
Author(s):  
Mingzhong Li ◽  
Liang Wan ◽  
Man-Kay Law ◽  
Li Meng ◽  
Yanwei Jia ◽  
...  
Keyword(s):  
High Resolution ◽  
High Resolution Melting ◽  
Digital Microfluidics ◽  
Melting Curve ◽  
High Accuracy ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Single Nucleotide ◽  
High Resolution Melting Curve ◽  
Microfluidics Platform

Proposed high-accuracy one-shot melting curve analysis on a digital microfluidics platform, enabling KRAS gene discrimination with single-nucleotide mutations in 3 seconds.

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